Personal information

Cryo-electron tomography, Single Particle Analysis, ribosome, structural biology
Germany

Biography

Julio Ortiz began his career in electron microscopy in Venezuela, under the supervision of Dr. Raúl Padrón at the Venezuelan Institute for Scientific Research (IVIC). He moved to Germany where he applied cryo-electron microscopy to actomyosin complexes supervised by Dr. Rasmus Schroeder in the Department of Dr. Ken Holmes (Max Plank Institute for Medical Research).
Turning his attention to tomography and associated techniques, Julio Ortiz went to the Max Planck Institute of Biochemistry in Martinsried for his post-doctoral studies in the dept. of Dr. Wolfgang Baumeister. He contributed to the development of an approach that proved one can obtain meaningful macromolecular structures by classifying and averaging subtomograms essentially moving single particle analysis from 2D to 3D. He presented the first ribosomal atlas of an intact cell (S. melliferum). Ortiz and coworkers revealed the native 3D organization of polysomes and solved the structure of hibernating ribosomes in vitro and inside intact bacterial cells. His expertise covers a broad range of techniques in cryo-electron microscopy, from single particle analysis to focused ion beam micromachinery for cellular tomography. After having served at the Netherlands Centre for Electron Nanoscopy (NeCEN) and the Institute of Genetics and of Molecular and Cellular Biology (IGBMC) , he joined the Forschungszentrum Jülich as Cryo-EM facility manager.

Activities

Employment (4)

Forschungszentrum Jülich: Julich, Nordrhein-Westfalen, DE

2018-10-01 to present | Cryo-EM Facility Manager (ER-C-3)
Employment
Source: Self-asserted source
Julio Ortiz

Institut de Génétique et de Biologie Moléculaire et Cellulaire: Strasbourg, Alsace, FR

2017-09-01 to 2018-09-30 | Head of EM Platform (Centre for Integrative Biology)
Employment
Source: Self-asserted source
Julio Ortiz

Universiteit Leiden: Leiden, Zuid-Holland, NL

2016-09-15 to 2017-08-31 | Scientific Operator (NeCEN)
Employment
Source: Self-asserted source
Julio Ortiz

Max-Planck-Institut für Biochemie: Martinsried, Bayern, DE

2002-09-01 to 2016-09-14 | Postdoctoral fellow / Project Leader (Structural Biology Department )
Employment
Source: Self-asserted source
Julio Ortiz

Education and qualifications (2)

Universidad Simón Bolivar: Caracas, Distrito Capital, VE

1997-11-01 to 2002-08-15 | Doctor in Biological Sciences (Biology)
Education
Source: Self-asserted source
Julio Ortiz

Universidad Simón Bolivar: Caracas, Distrito Capital, VE

1986-09-12 to 1992-06-30 | “Licenciado” in Biology (Biology)
Education
Source: Self-asserted source
Julio Ortiz

Works (10)

Transcription factors modulate RNA polymerase conformational equilibrium

Nature Communications
2022-03-22 | Journal article
Contributors: Chengjin Zhu; Xieyang Guo; Philippe Dumas; Maria Takacs; Mo’men Abdelkareem; Arnaud Vanden Broeck; Charlotte Saint-André; Gabor Papai; Corinne Crucifix; Julio Ortiz et al.
Source: check_circle
Crossref

Structural basis for safe and efficient energy conversion in a respiratory supercomplex

Nature Communications
2022-01-27 | Journal article
Contributors: Wei-Chun Kao; Claire Ortmann de Percin Northumberland; Tat Cheung Cheng; Julio Ortiz; Alexandre Durand; Ottilie von Loeffelholz; Oliver Schilling; Martin L. Biniossek; Bruno P. Klaholz; Carola Hunte
Source: check_circle
Crossref

Deep learning improves macromolecule identification in 3D cellular cryo-electron tomograms

Nature methods
2021 | Journal article
SOURCE-WORK-ID: FZJ-2022-00257
Part of ISSN: 1548-7091
HANDLE: 2128/29984
Contributors: Moebel, Emmanuel; Martinez-Sanchez, Antonio; Lamm, Lorenz; Righetto, Ricardo D.; Wietrzynski, Wojciech; Albert, Sahradha; Larivière, Damien; Fourmentin, Eric; Pfeffer, Stefan; Ortiz, Julio et al.
Source: check_circle
Forschungszentrum Jülich

PspA maintains membrane integrity by an ESCRT-like membrane remodeling activity

2020-09-23 | Other
Contributors: Benedikt Junglas; Stefan T. Huber; Thomas Heidler; Daniel Mann; Raoul Hennig; Mairi Clarke; Julio O. Ortiz; Dirk Schneider; Carsten Sachse
Source: check_circle
Crossref

Cryo-EM structure of the complete E. coli DNA gyrase nucleoprotein complex

Nature Communications
2019-10-30 | Journal article
Contributors: Arnaud Vanden Broeck; Christophe Lotz; Julio Ortiz; Valérie Lamour
Source: check_circle
Crossref

Cryo-EM structure of the complete E. coli DNA Gyrase nucleoprotein complex

2019-07-11 | Other
Contributors: Arnaud Vanden Broeck; Julio Ortiz; Valérie Lamour
Source: check_circle
Crossref

70 Three-dimensional arrangements of ribosomes inside fast growing E. coli cells.

2015 | Journal article
Contributors: Hoffmann T; Ortiz JO; Ulrich Hartl F; Baumeister W
Source: Self-asserted source
Julio Ortiz via Europe PubMed Central

Structure of hibernating ribosomes studied by cryoelectron tomography in vitro and in situ.

2010-08 | Journal article
Contributors: Ortiz JO; Brandt F; Matias VR; Sennels L; Rappsilber J; Scheres SH; Eibauer M; Hartl FU; Baumeister W
Source: Self-asserted source
Julio Ortiz via Europe PubMed Central

A movie of the RNA polymerase nucleotide addition cycle.

2009-06 | Journal article
Contributors: Brueckner F; Ortiz J; Cramer P
Source: Self-asserted source
Julio Ortiz via Europe PubMed Central

The native 3D organization of bacterial polysomes.

2009-01 | Journal article
Contributors: Brandt F; Etchells SA; Ortiz JO; Elcock AH; Hartl FU; Baumeister W
Source: Self-asserted source
Julio Ortiz via Europe PubMed Central