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Receptor trafficking, tumor heterogeneity, iron transport, FRET, FLIM, endosomes
United States

Biography

Margarida Barroso is a Professor at the Department of Molecular and Cellular Physiology, Albany Medical College in Albany, New York. She received her Ph.D. in Genetics from the University of Lisbon/Gulbenkian Institute of Sciences in Portugal and was a post-doctoral fellow at the Department of Molecular Biology, Princeton University. She is a faculty instructor in several international imaging courses and has two issued patents on FRET imaging technology. Dr. Barroso is currently the Past-Past-President of the Histochemical Society.

Activities

Employment (4)

Albany Medical Center Hospital: Albany, New York, US

2022-06-01 to present | Director of the Imaging Core Facility (Molecular and Cellular Physiology)
Employment
Source: Self-asserted source
Margarida Barroso

Rensselaer Polytechnic Institute: Troy, NY, US

2019-10-01 to present | Adjunct Professor (Biomedical Engineering)
Employment
Source: Self-asserted source
Margarida Barroso

Albany Medical College: Albany, NY, US

2018-11-15 to present | Full Professor with tenure (Molecular and Cellular Physiology)
Employment
Source: Self-asserted source
Margarida Barroso

Albany Medical College: Albany, NY, US

2014-09-01 to 2018-11-14 | Associate Professor (Molecular and Cellular Physiology)
Employment
Source: Self-asserted source
Margarida Barroso

Education and qualifications (3)

Princeton University: Princeton, NJ, US

1991-04-01 to 1994-06-31 | Post-doctoral research associate (Molecular Biology)
Education
Source: Self-asserted source
Margarida Barroso

University of Lisbon: Lisbon, PT

1986-09-01 to 1991-03-31 | PhD (Faculty of Sciences)
Education
Source: Self-asserted source
Margarida Barroso

University of Lisbon: Lisbon, PT

1981-10-01 to 1986-07-31 | Licenciatura in Biology (5 years) (Faculty of Sciences)
Education
Source: Self-asserted source
Margarida Barroso

Professional activities (3)

SPIE: Bellingham, WA, US

Member
Membership
Source: Self-asserted source
Margarida Barroso

Histochemical Society: Bethesda, MD, US

Past-President
Membership
Source: Self-asserted source
Margarida Barroso

American Society for Cell Biology: Bethesda, MD, US

Membership
Source: Self-asserted source
Margarida Barroso

Funding (10)

AI enhanced lifetime-based mesoscopic in vivo imaging of tissue molecular heterogeneity

2023-06-01 to 2028-05-31 | Grant
National Cancer Institute (Bethesda, US)
GRANT_NUMBER: R01CA271371
Source: Self-asserted source
Margarida Barroso via DimensionsWizard

Artificial intelligence enhanced cancer cell classification based organelle morphology and topology

2022-09-01 to 2024-08-31 | Grant
National Cancer Institute (Bethesda, US)
GRANT_NUMBER: R21CA274622
Source: Self-asserted source
Margarida Barroso via DimensionsWizard

In vivo Macroscopic Fluorescence Lifetime Molecular Optical Imaging

2020-05-01 to 2024-04-30 | Grant
National Cancer Institute (Rockville, US)
GRANT_NUMBER: R01CA250636
Source: Self-asserted source
Margarida Barroso via DimensionsWizard

Endosome-mitochondria interactions in breast cancer cells

2020-02-01 to 2025-01-31 | Grant
National Cancer Institute (Rockville, US)
GRANT_NUMBER: R01CA233188
Source: Self-asserted source
Margarida Barroso via DimensionsWizard

Photon-counting X-ray and Optical Tomography for Preclinical Cancer Research

2019-09-12 to 2024-08-31 | Grant
National Cancer Institute (Rockville, US)
GRANT_NUMBER: R01CA237267
Source: Self-asserted source
Margarida Barroso via DimensionsWizard

Role of tumor heterogeneity on receptor engagement

2017-04-01 to 2023-03-31 | Grant
National Cancer Institute (Bethesda, US)
GRANT_NUMBER: R01CA207725
Source: Self-asserted source
Margarida Barroso via DimensionsWizard

ROLE OF TUMOR HETEROGENEITY ON RECEPTOR ENGAGEMENT

2017-04 to 2022-03 | Award
NIH Office of the Director -NCI (MD, MD, US)
GRANT_NUMBER: 1R01CA207725-01A1
Source: Self-asserted source
Margarida Barroso

Time-Resolved Wide-Field Molecular Optical Tomography

2015-04 to 2019-01 | Award
NIH Office of the Director - NIBIB (MD, MD, US)
Part of GRANT_NUMBER: 5 R01 EB019443-03
Source: Self-asserted source
Margarida Barroso

The 3D whole Body Distribution of NIR transferrin using FRET tomography imaging

Source: Self-asserted source
Margarida Barroso via DimensionsWizard

REGULATION OF CYTOSKELETON AND MEMBRANE TRAFFIC BY P22

1998-05-01 to 2005-04-30 | Grant
National Institute of General Medical Sciences (Bethesda, US)
GRANT_NUMBER: R01GM057519
Source: Self-asserted source
Margarida Barroso via DimensionsWizard

Works (50 of 114)

Items per page:
Page 1 of 3

in vivo quantitative FRET small animal imaging: intensity versus lifetime-based FRET

figshare
2023 | Dataset
Contributors: Jason T. Smith; Nattawut Sinsuebphon; Xavier Michalet; Alena Rudkouskaya; Xavier Intes; Margarida Barroso
Source: check_circle
DataCite

Probing organoid metabolism using fluorescence lifetime imaging microscopy (FLIM): The next frontier of drug discovery and disease understanding.

Advanced drug delivery reviews
2023-08 | Journal article
Contributors: Barroso M; Monaghan MG; Niesner R; Dmitriev RI
Source: Self-asserted source
Margarida Barroso via Europe PubMed Central

Spatial topology of organelle is a new breast cancer cell classifier.

iScience
2023-06 | Journal article
Contributors: Wang L; Goldwag J; Bouyea M; Barra J; Matteson K; Maharjan N; Eladdadi A; Embrechts MJ; Intes X; Kruger U et al.
Source: Self-asserted source
Margarida Barroso via Europe PubMed Central

<i>INKILN</i> is a Novel Long Noncoding RNA Promoting Vascular Smooth Muscle Inflammation via Scaffolding MKL1 and USP10.

Circulation
2023-05 | Journal article
Contributors: Zhang W; Zhao J; Deng L; Ishimwe N; Pauli J; Wu W; Shan S; Kempf W; Ballantyne MD; Kim D et al.
Source: Self-asserted source
Margarida Barroso via Europe PubMed Central

In vivo quantitative FRET small animal imaging: Intensity versus lifetime-based FRET.

Biophysical reports
2023-05 | Journal article
Contributors: Smith JT; Sinsuebphon N; Rudkouskaya A; Michalet X; Intes X; Barroso M
Source: Self-asserted source
Margarida Barroso via Europe PubMed Central

Nuclear cytoglobin associates with HMGB2 and regulates DNA damage and genome-wide transcriptional output in the vasculature

2023-05 | Preprint
Contributors: Mathai C; Jourd’heuil F; Pham LGC; Gilliard K; Howard D; Balnis J; Jaitovich A; Chittur SV; Rilley M; Peredo-Wende R et al.
Source: Self-asserted source
Margarida Barroso via Europe PubMed Central

Data for "In vitro and in vivo NIR Fluorescence Lifetime Imaging with a time-gated SPAD camera"

figshare
2022 | Dataset
Contributors: Xavier Michalet; Jason Smith; Xavier Intes; shimon weiss; Margarida Barroso; Alena Rudkouskaya; Arin Can Ulku; Claudio Bruschini; Edoardo Charbon; Juhi Munmun Gupta
Source: check_circle
DataCite

Quantitative label-free imaging of iron-bound transferrin in breast cancer cells and tumors

Redox Biology
2020-09 | Journal article
Part of ISSN: 2213-2317
Source: Self-asserted source
Margarida Barroso

Complex Rab4-Mediated Regulation of Endosomal Size and EGFR Activation

Molecular Cancer Research
2020-05 | Journal article
Part of ISSN: 1541-7786
Part of ISSN: 1557-3125
Source: Self-asserted source
Margarida Barroso

Phosphorylation of GMFγ by c-Abl coordinates lamellipodial and focal adhesion dynamics to regulate airway smooth muscle cell migration.pdf

Figshare
2019 | Other
Contributors: Brennan Gerlach; Kate Tubbesing; Guoning Liao; Alyssa C. Rezey; Ruping Wang; Margarida Barroso; Dale Tang
Source: check_circle
DataCite

Fast fit-free analysis of fluorescence lifetime imaging via deep learning.

Proceedings of the National Academy of Sciences of the United States of America
2019-11-12 | Journal article
Source: Self-asserted source
Margarida Barroso

Phosphorylation of GMFγ by c-Abl Coordinates Lamellipodial and Focal Adhesion Dynamics to Regulate Airway Smooth Muscle Cell Migration.

American journal of respiratory cell and molecular biology
2019-08-01 | Journal article
Source: Self-asserted source
Margarida Barroso

Cardiomyocyte orientation modulated by the Numb family proteins-N-cadherin axis is essential for ventricular wall morphogenesis.

Proceedings of the National Academy of Sciences of the United States of America
2019-07-12 | Journal article
Source: Self-asserted source
Margarida Barroso

Resolvin D1 promotes the targeting and clearance of necroptotic cells.

Cell death and differentiation
2019-06-20 | Journal article
Source: Self-asserted source
Margarida Barroso

Laser-based 3D bioprinting for spatial and size control of tumor spheroids and embryoid bodies.

Acta biomaterialia
2019-02-15 | Journal article
Source: Self-asserted source
Margarida Barroso

Supporting Data for Fig. 2 &amp; S1 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using near-infrared dyes"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 2 &amp; S1 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using near-infrared dyes"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 3 &amp; S2 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using near-infrared dyes"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 3 &amp; S2 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 3 &amp; S2 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 4 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using near-infrared dyes"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 4 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 4 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 5 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using near-infrared dyes"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 5 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 5 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 6, 7 &amp; S3 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using near-infrared dyes"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 6, 7 &amp; S3 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 6, 7 &amp; S3-S6 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 6, 7 &amp; S3-S6 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Data for Fig. 6, 7 &amp; S6 of "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Margarida Barroso; Nattawut Sinsuebphon; Sez-Jade Chen; Alena Rudkouskaya; Xavier Intes
Source: check_circle
DataCite

Supporting Movie 1

Figshare
2018 | Other
Contributors: Xavier Michalet; Sez-Jade Chen; Nattawut Sinsuebphon; Alena Rudkouskaya; Margarida Barroso; Xavier Intes
Source: check_circle
DataCite

Supporting Movie 1 for "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using near-infrared dyes"

Figshare
2018 | Other
Contributors: Xavier Michalet; Sez-Jade Chen; Nattawut Sinsuebphon; Alena Rudkouskaya; Margarida Barroso; Xavier Intes
Source: check_circle
DataCite

Supporting Movie 1 for "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Sez-Jade Chen; Nattawut Sinsuebphon; Alena Rudkouskaya; Margarida Barroso; Xavier Intes
Source: check_circle
DataCite

Supporting Movie 1 for "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Sez-Jade Chen; Nattawut Sinsuebphon; Alena Rudkouskaya; Margarida Barroso; Xavier Intes
Source: check_circle
DataCite

Supporting Movie 2 for "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using near-infrared dyes"

Figshare
2018 | Other
Contributors: Xavier Michalet; Sez-Jade Chen; Nattawut Sinsuebphon; Alena Rudkouskaya; Margarida Barroso; Xavier Intes
Source: check_circle
DataCite

Supporting Movie 2 for "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Sez-Jade Chen; Nattawut Sinsuebphon; Alena Rudkouskaya; Margarida Barroso; Xavier Intes
Source: check_circle
DataCite

Supporting Movie 2 for "In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging"

Figshare
2018 | Other
Contributors: Xavier Michalet; Sez-Jade Chen; Nattawut Sinsuebphon; Alena Rudkouskaya; Margarida Barroso; Xavier Intes
Source: check_circle
DataCite

In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short-lifetime near-infrared dyes and time-gated imaging.

Journal of biophotonics
2018-12-09 | Journal article
Source: Self-asserted source
Margarida Barroso

Quantitative imaging of receptor-ligand engagement in intact live animals.

Journal of controlled release : official journal of the Controlled Release Society
2018-07-20 | Journal article
Source: Self-asserted source
Margarida Barroso

Comparison of illumination geometry for lifetime-based measurements in whole-body preclinical imaging.

Journal of biophotonics
2018-06-28 | Journal article
Source: Self-asserted source
Margarida Barroso

AlliGator: A Phasor Computational Platform for Fast in vivo Lifetime Analysis

Optics in the Life Sciences Congress
2017 | Conference paper
Contributors: Sez-Jade Chen; Nattawut Sinsuebphon; Margarida Barroso; Xavier Intes; Xavier Michalet
Source: Self-asserted source
Margarida Barroso via Crossref Metadata Search

Raman Spectroscopic Analysis of Iron Transport across Membranes in Cells

Optics in the Life Sciences Congress
2017 | Conference paper
Contributors: Anupam Das; Kate Tubbesing; Alexander Khmaladze; Margarida Barroso; Anna Sharikova
Source: Self-asserted source
Margarida Barroso via Crossref Metadata Search

TOWARD BAYESIAN INFERENCE OF THE SPATIAL DISTRIBUTION OF PROTEINS FROM THREE-CUBE FÖRSTER RESONANCE ENERGY TRANSFER DATA.

The annals of applied statistics
2017-09-01 | Journal article
Source: Self-asserted source
Margarida Barroso

Golgi-Associated Protein Kinase C-ε Is Delivered to Phagocytic Cups: Role of Phosphatidylinositol 4-Phosphate.

Journal of immunology (Baltimore, Md. : 1950)
2017-05-24 | Journal article
Source: Self-asserted source
Margarida Barroso

Fluorescence lifetime FRET non-invasive imaging of breast cancer xenografts provides a measure of target engagement in vivo (Conference Presentation)

Molecular-Guided Surgery: Molecules, Devices, and Applications III
2017-04 | Conference paper
Contributors: Alena Rudkouskaya; Nattawut Sinsuebphon; Xavier Intes; Margarida Barroso; Brian W. Pogue; Sylvain Gioux
Source: Self-asserted source
Margarida Barroso via Crossref Metadata Search

Fluorescence lifetime FRET imaging of receptor-ligand complexes in tumor cells in vitro and in vivo

Multiphoton Microscopy in the Biomedical Sciences XVII
2017-02 | Conference paper
Contributors: Alena Rudkouskaya; Nattawut Sinsuebphon; Xavier Intes; Joseph E. Mazurkiewicz; Margarida Barroso; Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie
Source: Self-asserted source
Margarida Barroso via Crossref Metadata Search

Fluorescence Lifetime-based Multiplexing of Near-Infrared Förster Resonance Energy Transfer Pairs

Biomedical Optics 2016
2016 | Conference paper
Contributors: Sez-Jade Chen; Nattawut Sinsuebphon; Alena Rudkouskaya; Margarida Barroso; Xavier Intes
Source: Self-asserted source
Margarida Barroso via Crossref Metadata Search

Role of Tumor Heterogeneity in Imaging Breast Cancer Targeted Delivery using FLIM FRET in Vivo<br />

Biomedical Optics 2016
2016 | Conference paper
Contributors: Alena Rudkouskaya; Nattawut Sinsuebphon; Xavier Intes; Margarida Barroso
Source: Self-asserted source
Margarida Barroso via Crossref Metadata Search

Whole body lifetime FRET imaging in transmission and reflectance for the assessment of drug delivery efficacy in small animals

Biomedical Optics 2016
2016 | Conference paper
Contributors: Nattawut Sinsuebphon; Alena Rudkouskaya; Margarida Barroso; Xavier Intes
Source: Self-asserted source
Margarida Barroso via Crossref Metadata Search
Items per page:
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Peer review (1 review for 1 publication/grant)

Review activity for PloS one. (1)