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optogenetics, optical mapping, bioelectricity, biophotonics, cardiac, modeling
United States

Biography

Professor Emilia Entcheva is a biomedical scientist and engineer. She directs the Cardiac Optogenetics & Optical Imaging Laboratory (at George Washington University, Washington DC) focused on advancement of personalized medicine by innovative engineering. We combine biophotonics tools (optogenetics-based actuation and advanced optical mapping) with stem-cell-derived cardiomyocyte technology, tissue engineering and gene-modulation approaches (CRISPRi/CRISPRa) to design novel massively parallel platforms for high-throughput functional screening in human patient-derived cells. Machine learning (AI) is applied to capture key biophysical signatures in the diverse human cell responses and build better population-and patient-specific predictors of drug action. All-optical electrophysiology and computation are used for real-time feedback control of complex biological processes. Our work is funded by the National Science Foundation and the National Institutes of Health.

Activities

Employment (3)

The George Washington University: Washington, DC, US

2016-01-01 to present | Professor (Biomedical Engineering)
Employment
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Emilia Entcheva

Stony Brook University: Stony Brook, NY, US

2001-08-01 to 2016-01-01 | Assistant, Associat and Full Professor (Biomedical Engineering)
Employment
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Emilia Entcheva

Johns Hopkins University: Baltimore, Maryland, US

1998-09-01 to 2001-08-01 | Postdoctoral Fellows (Biomedical Engineering)
Employment
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Emilia Entcheva

Works (50 of 84)

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Paradoxical effects of Zim3, a CRISPRi effector, on human iPSC-cardiomyocyte electrophysiology

PNAS Nexus
2024-01-20 | Journal article | Author
Part of ISSN: 2752-6542
Contributors: Julie Leann Han; Yuli W Heinson; Maria R. Pozo; Li Weizhen; Emilia Entcheva
Source: Self-asserted source
Emilia Entcheva

Gene Modulation with CRISPR-based Tools in Human iPSC-Cardiomyocytes

Stem Cell Reviews and Reports
2023 | Journal article
Contributors: Han, Julie Leann; Entcheva, Emilia
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Portable low-cost macroscopic mapping system for all-optical cardiac electrophysiology

Journal of Biomedical Optics
2023 | Journal article
Contributors: Heinson, Yuli W. W.; Han, Julie L. L.; Entcheva, Emilia
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Simultaneous Widefield Voltage and Dye-Free Optical Mapping Quantifies Electromechanical Waves in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

ACS Photonics
2023 | Journal article
Contributors: Liu, Wei; Han, Julie L.; Tomek, Jakub; Bub, Gil; Entcheva, Emilia
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OptoDyCE-plate as an affordable high throughput imager for all optical cardiac electrophysiology

Journal of Molecular and Cellular Cardiology Plus
2023-12 | Journal article
Part of ISSN: 2772-9761
Contributors: Yuli W. Heinson; Julie L. Han; Emilia Entcheva
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Emilia Entcheva

CRISPRi gene modulation and all-optical electrophysiology in post-differentiated human iPSC-cardiomyocytes

Communications Biology
2023-12-07 | Journal article | Author
Part of ISSN: 2399-3642
Contributors: Julie L. Han; Yuli W. Heinson; Christianne J. Chua; Wei Liu; Emilia Entcheva
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Emilia Entcheva

Opto-SICM framework combines optogenetics with scanning ion conductance microscopy for probing cell-to-cell contacts

Communications Biology
2023-11-08 | Journal article | Author
Part of ISSN: 2399-3642
Contributors: Qianqian Song; Anita Alvarez-Laviada; Sarah E. Schrup; Benedict Reilly-O'Donnell; Emilia Entcheva; Julia Gorelik
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Emilia Entcheva

Outcomes of hypothalamic oxytocin neuron-driven cardioprotection after acute myocardial infarction

Basic Research in Cardiology
2023-10-06 | Journal article
Part of ISSN: 1435-1803
Contributors: Kathryn J. Schunke; Jeannette Rodriguez; Jhansi Dyavanapalli; John Schloen; Xin Wang; Joan Escobar; Grant Kowalik; Emily C. Cheung; Caitlin Ribeiro; Rebekah Russo et al.
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Emilia Entcheva

High-throughput optical sensing of peri-cellular oxygen in cardiac cells: system characterization, calibration, and testing

Frontiers in Bioengineering and Biotechnology
2023-06-16 | Journal article
Part of ISSN: 2296-4185
Contributors: Weizhen Li; David McLeod; John T. Ketzenberger; Grant Kowalik; Rebekah Russo; Zhenyu Li; Matthew W. Kay; Emilia Entcheva
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Emilia Entcheva

Optogenetics for light control of biological systems

Nature Reviews Methods Primers
2022 | Journal article
Contributors: Emiliani, Valentina; Entcheva, Emilia; Hedrich, Rainer; Hegemann, Peter; Konrad, Kai R.; Luscher, Christian; Mahn, Mathias; Pan, Zhuo-Hua; Sims, Ruth R.; Vierock, Johannes et al.
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Protein and mRNA Quantification in Small Samples of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates

Methods in Molecular Biology
2022 | Journal article
Contributors: Weizhen Li; Julie L. Han; Emilia Entcheva
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Sex-dependent transcription of cardiac electrophysiology and links to acetylation modifiers based on the GTEx database

Frontiers in Cardiovascular Medicine
2022 | Journal article
Contributors: Pressler, Michael P.; Horvath, Anelia; Entcheva, Emilia
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Human iPSC-Cardiomyocytes as an Experimental Model to Study Epigenetic Modifiers of Electrophysiology

Cells
2022-01 | Journal article | Author
Contributors: Maria R. Pozo; Gantt Meredith; Emilia Entcheva
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Cardiac optogenetics: a decade of enlightenment

Nature Reviews Cardiology
2021 | Journal article
Contributors: Entcheva, Emilia; Kay, Matthew W.
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Integration of Engineered "Spark-Cell" Spheroids for Optical Pacing of Cardiac Tissue

Frontiers in Bioengineering and Biotechnology
2021 | Journal article
Contributors: Chua, Christianne J.; Han, Julie L.; Li, Weizhen; Liu, Wei; Entcheva, Emilia
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Novel Optics-Based Approaches for Cardiac Electrophysiology: A Review

Frontiers in Physiology
2021 | Journal article
Contributors: Muellenbroich, M. Caroline; Kelly, Allen; Acker, Corey; Bub, Gil; Bruegmann, Tobias; Di Bona, Anna; Entcheva, Emilia; Ferrantini, Cecilia; Kohl, Peter; Lehnart, Stephan E. et al.
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OptoGap is an optogenetics-enabled assay for quantification of cell-cell coupling in multicellular cardiac tissue

Scientific Reports
2021 | Journal article
Contributors: Boyle, Patrick M.; Yu, Jinzhu; Klimas, Aleksandra; Williams, John C.; Trayanova, Natalia A.; Entcheva, Emilia
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Optogenetic current in myofibroblasts acutely alters electrophysiology and conduction of co-cultured cardiomyocytes

Scientific Reports
2021 | Journal article
Contributors: Kostecki, Geran M.; Shi, Yu; Chen, Christopher S.; Reich, Daniel H.; Entcheva, Emilia; Tung, Leslie
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All-Optical Electrophysiology Refines Populations of In Silico Human iPSC-CMs for Drug Evaluation

Biophysical Journal
2020 | Journal article
Contributors: Paci, Michelangelo; Passini, Elisa; Klimas, Aleksandra; Severi, Stefano; Hyttinen, Jari; Rodriguez, Blanca; Entcheva, Emilia
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Microfluidics-enabled 96-well perfusion system for high-throughput tissue engineering and long-term all-optical electrophysiology

Lab on a Chip
2020 | Journal article
Contributors: Wei, Lai; Li, Weizhen; Entcheva, Emilia; Li, Zhenyu
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Multimodal on-axis platform for all-optical electrophysiology with near-infrared probes in human stem-cell-derived cardiomyocytes

Progress in Biophysics and Molecular Biology
2020 | Journal article
Contributors: Klimas, Aleksandra; Ortiz, Gloria; Boggess, Steven C.; Miller, Evan W.; Entcheva, Emilia
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Optical Interrogation of Sympathetic Neuronal Effects on Macroscopic Cardiomyocyte Network Dynamics

IScience
2020 | Journal article
Contributors: Burton, Rebecca-Ann B.; Tomek, Jakub; Ambrosi, Christina M.; Larsen, Hege E.; Sharkey, Amy R.; Capel, Rebecca A.; Corbett, Alexander D.; Bilton, Samuel; Klimas, Aleksandra; Stephens, Guy et al.
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Syncytium cell growth increases Kir2.1 contribution in human iPSC-cardiomyocytes

American Journal of Physiology-Heart and Circulatory Physiology
2020 | Journal article
Contributors: Li, Weizhen; Han, Julie L.; Entcheva, Emilia
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Adeno-Associated Virus Mediated Gene Delivery: Implications for Scalable in vitro and in vivo Cardiac Optogenetic Models

Frontiers in Physiology
2019 | Journal article
Contributors: Ambrosil, Christina M.; Sadanandal, Gouri; Han, Ulie L.; Entcheva, Emilia
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Cardiomyocyte-myofibroblast contact dynamism is modulated by connexin-43

The FASEB Journal
2019 | Journal article
Contributors: Schultz, Francisca; Swiatlowska, Pamela; Alvarez-Laviada, Anita; Sanchez-Alonso, Jose L.; Song, Qianqian; de Vries, Antoine A. F.; Pijnappels, Daniel A.; Ongstad, Emily; Braga, Vania M. M.; Entcheva, Emilia et al.
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Light-Activated Dynamic Clamp Using iPSC-Derived Cardiomyocytes

Biophysical Journal
2018 | Journal article
Contributors: Quach, Bonnie; Krogh-Madsen, Trine; Entcheva, Emilia; Christini, David J.
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Ultrasound-Induced Insulin Release as a Potential Novel Treatmentfor Type 2 Diabetes Mellitus

Annual International Conference of the IEEE Engineering in Medicine and Biology
2018 | Conference paper
Contributors: Singh, Tania; Castellanos, Ivan Suarez; Haar, Shane; Klimas, Aleks; Entcheva, Emilia; Salvador, Tyler; Bhowmick, Diti Chatterjee; Cohen, Joshua; Cleary, Kevin; Jeremic, Aleksandar et al.
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Uncovering an electrically heterogeneous cardiomyocyte by FRAP-quantified diffusion in the T-tubules

Proceedings of the National Academy of Sciences
2018 | Journal article
Contributors: Entcheva, Emilia
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All-optical control of cardiac excitation: combined high-resolution optogenetic actuation and optical mapping

The Journal of Physiology
2016 | Journal article
Contributors: Entcheva, Emilia; Bub, Gil
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Inscribing Optical Excitability to Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary Cardiac Fibroblasts

Methods in Molecular Biology
2016 | Journal article
Contributors: Yu, Jinzhu; Entcheva, Emilia
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OptoDyCE as an automated system for high-throughput all-optical dynamic cardiac electrophysiology

Nature Communications
2016 | Journal article
Contributors: Klimas, Aleksandra; Ambrosi, Christina M.; Yu, Jinzhu; Williams, John C.; Bien, Harold; Entcheva, Emilia
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A step closer to cardiac optogenetics in vivo

Cardiovascular Research
2015 | Journal article
Contributors: Entcheva, Emilia
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Cardiac Optogenetics: Enhancement by All-trans-Retinal

Scientific Reports
2015 | Journal article
Contributors: Yu, Jinzhu; Chen, Kay; Lucero, Rachel V.; Ambrosi, Christina M.; Entcheva, Emilia
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Computational modeling of cardiac optogenetics: Methodology overview & review of findings from simulations

Computers in Biology and Medicine
2015 | Journal article
Contributors: Boyle, Patrick M.; Karathanos, Thomas V.; Entcheva, Emilia; Trayanova, Natalia A.
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Optical control of excitation waves in cardiac tissue

Nature Photonics
2015 | Journal article
Contributors: Burton, Rebecca A. B.; Klimas, Aleksandra; Ambrosi, Christina M.; Tomek, Jakub; Corbett, Alex; Entcheva, Emilia; Bub, Gil
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Optogenetic versus Electrical Stimulation of Human Cardiomyocytes: Modeling Insights

Biophysical Journal
2015 | Journal article
Contributors: Williams, John C.; Entcheva, Emilia
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Calpain-Dependent Cleavage of Junctophilin-2 and T-Tubule Remodeling in a Mouse Model of Reversible Heart Failure

Journal of the American Heart Association
2014 | Journal article
Contributors: Wu, Chia-Yen C.; Chen, Biyi; Jiang, Ya-Ping; Jia, Zhiheng; Martin, Dwight W.; Liu, Shengnan; Entcheva, Emilia; Song, Long-Sheng; Lin, Richard Z.
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Cardiac applications of optogenetics

Progress in Biophysics and Molecular Biology
2014 | Journal article
Contributors: Ambrosi, Christina M.; Klimas, Aleksandra; Yu, Jinzhu; Entcheva, Emilia
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Channelrhodopsin2 Current During the Action Potential: "Optical AP Clamp'' and Approximation

Scientific Reports
2014 | Journal article
Contributors: Entcheva, Emilia; Williams, John C.
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Fiat lux in understanding cardiac pacing, resynchronization and signalling by way of optogenetics

Cardiovascular Research
2014 | Journal article
Contributors: Entcheva, Emilia
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See the light: can optogenetics restore healthy heartbeats? And, if it can, is it really worth the effort?

Expert Review of Cardiovascular Therapy
2014 | Journal article
Contributors: Boyle, Patrick M.; Entcheva, Emilia; Trayanova, Natalia A.
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Toward microendoscopy-inspired cardiac optogenetics in vivo: technical overview and perspective

Journal of Biomedical Optics
2014 | Journal article
Contributors: Klimas, Aleksandra; Entcheva, Emilia
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A comprehensive multiscale framework for simulating optogenetics in the heart

Nature Communications
2013 | Journal article
Contributors: Boyle, Patrick M.; Williams, John C.; Ambrosi, Christina M.; Entcheva, Emilia; Trayanova, Natalia A.
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Cardiac optogenetics

American Journal of Physiology-Heart and Circulatory Physiology
2013 | Journal article
Contributors: Entcheva, Emilia
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Computational Optogenetics: Empirically-Derived Voltage- and Light-Sensitive Channelrhodopsin-2 Model

PLOS Computational Biology
2013 | Journal article
Contributors: Williams, John C.; Xu, Jianjin; Lu, Zhongju; Klimas, Aleksandra; Chen, Xuxin; Ambrosi, Christina M.; Cohen, Ira S.; Entcheva, Emilia
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Cardiac Cellular Coupling and the Spread of Early Instabilities in Intracellular Ca2+

Biophysical Journal
2012 | Journal article
Contributors: Jia, Zhiheng; Bien, Harold; Shiferaw, Yohannes; Entcheva, Emilia
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Effect of Skeletal Muscle Na<SUP>+</SUP> Channel Delivered Via a Cell Platform on Cardiac Conduction and Arrhythmia Induction

Circulation: Arrhythmia and Electrophysiology
2012 | Journal article
Contributors: Boink, Gerard J. J.; Lu, Jia; Driessen, Helen E.; Duan, Lian; Sosunov, Eugene A.; Anyukhovsky, Evgeny P.; Shlapakova, Iryna N.; Lau, David H.; Rosen, Tove S.; Danilo, Peter et al.
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Improving Cardiac Conduction With a Skeletal Muscle Sodium Channel by Gene and Cell Therapy

Journal of Cardiovascular Pharmacology
2012 | Journal article
Contributors: Lu, Jia; Wang, Hong-Zhan; Jia, Zhiheng; Zuckerman, Joan; Lu, Zhongju; Guo, Yuanjian; Boink, Gerard J. J.; Brink, Peter R.; Robinson, Richard B.; Entcheva, Emilia et al.
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Hypertrophic phenotype in cardiac cell assemblies solely by structural cues and ensuing self-organization

The FASEB Journal
2011 | Journal article
Contributors: Chung, Chiung-yin; Bien, Harold; Sobie, Eric A.; Dasari, Vikram; McKinnon, David; Rosati, Barbara; Entcheva, Emilia
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M-3: Microscope-based maskless micropatterning with dry film photoresist

Biomedical Microdevices
2011 | Journal article
Contributors: Leigh, Steven Y.; Tattu, Aashay; Mitchell, Joseph S. B.; Entcheva, Emilia
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Peer review (2 reviews for 2 publications/grants)

Review activity for Nature (1)
Review activity for Nature communications (1)