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Employment (6)

Charles University: Prague, CZ

2021-03-01 to present | Group leader (1st Faculty of Medicine)
Employment
Source: Self-asserted source
Josef Lazar

Institute of Organic Chemistry and Biochemistry: Prague, CZ

2021-01-01 to present | Senior Research Scientist
Employment
Source: Self-asserted source
Josef Lazar

University of South Bohemia: Budweis, CZ

2007-09-01 to 2021-05-31 | lab head (Dept. of Molecular Biology)
Employment
Source: Self-asserted source
Josef Lazar

Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences: Prague, CZ

2014-09-01 to 2020-12-31 | Head, Laboratory of Advanced Optical Microscopy
Employment
Source: Self-asserted source
Josef Lazar

Institute of Microbiology, Czech Academy of Sciences: Nove Hrady, CZ

2007-07-01 to 2020-12-31 | Research Scientist (Center for nanobiology and structural biology)
Employment
Source: Self-asserted source
Josef Lazar

Columbia University: New York, New York, US

2002 to 2007 | Postdoc/Columbia Science Fellow (Biological Sciences)
Employment
Source: Self-asserted source
Josef Lazar

Education and qualifications (2)

University of Utah: Salt Lake City, Utah, US

1996 to 2001 | Ph.D. (Medicinal Chemistry)
Education
Source: Self-asserted source
Josef Lazar

Charles University: Prague, CZ

1993 to 1996 | M.S. (Organic Chemistry)
Education
Source: Self-asserted source
Josef Lazar

Works (49)

Directionality of two-photon absorption in representative fluorescent proteins

2025-01-01 | Preprint
Contributors: Josef Lazar; Štěpán Timr; Olga Rybakova; Jiří Brynda; Adam Thuen; Mikhail Drobizhev; Jitka Myšková
Source: check_circle
Crossref

FLIPs: Novel Genetically Encoded Biosensors for Functional Imaging of Cell Signaling by Polarization Microscopy

2024-02-26 | Preprint
Contributors: Paul Miclea; Vendula Nagy-Marková; Robin Van den Eynde; Wim Vandenberg; Alina Sakhi; Alexey Bondar; Jitka Myšková; Peter Dedecker; Josef Lazar
Source: check_circle
Crossref

Dynamics of transition dipole moment orientation in representative fluorescent proteins

Physical Chemistry Chemical Physics
2023 | Journal article
Contributors: Petro Khoroshyy; Hector Martinez-Seara; Jitka Myšková; Josef Lazar
Source: check_circle
Crossref
grade
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The TriScan: fast and sensitive 3D confocal fluorescence imaging using a simple optical design

2023-04-11 | Preprint
Contributors: Robin Van den Eynde; Jon Verheyen; Paul Miclea; Josef Lazar; Wim Vandenberg; Peter Dedecker
Source: check_circle
Crossref

Directionality of light absorption and emission in representative fluorescent proteins (vol 117, pg 32395, 2020)

Proceedings of the National Academy of Sciences
2022 | Journal article
Contributors: Myskova, Jitka; Rybakova, Olga; Brynda, Jiri; Khoroshyy, Petro; Bondar, Alexey; Lazar, Josef
Source: check_circle
Web of Science Researcher Profile Sync

Dynamics of transition dipole orientation in representative fluorescent proteins

Biophysical Journal
2022 | Journal article
Contributors: Lazar, Josef; Khoroshyy, Petro; Myskova, Jitka; Martinez-Seara, Hector
Source: check_circle
Web of Science Researcher Profile Sync

Imaging GPCR signaling by polarization microscopy

Biophysical Journal
2022 | Journal article
Contributors: Miclea, Paul S.; Markova, Vendula; Vaitova, Zuzana; Bondar, Alexey; Lazar, Josef
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Directionality of Light Absorption and Emission in Fluorescent Proteins

Biophysical Journal
2021 | Journal article
Contributors: Myskova, Jitka; Rybakova, Olga; Brynda, Jiri; Khoroshyy, Petro; Bondar, Alexey; Lazar, Josef
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Web of Science Researcher Profile Sync

Observing Tyrosine Kinase Function by Polarization Resolved Fluorescence Microscopy

Biophysical Journal
2021 | Journal article
Contributors: Sakhi, Alina; Lazar, Josef
Source: check_circle
Web of Science Researcher Profile Sync

Polarization Microscopy Imaging of G Proteins at Endogenous Expression Levels

Biophysical Journal
2021 | Journal article
Contributors: Miclea, Paul S.; Lazar, Josef
Source: check_circle
Web of Science Researcher Profile Sync

Quantitative linear dichroism imaging of molecular processes in living cells made simple by open software tools

Communications Biology
2021 | Journal article
Contributors: Bondar, Alexey; Rybakova, Olga; Melcr, Josef; Dohnalek, Jan; Khoroshyy, Petro; Tichacek, Ondrej; Timr, Stepan; Miclea, Paul; Sakhi, Alina; Markova, Vendula et al.
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Web of Science Researcher Profile Sync

Using fluorescent protein directionality in microscopy imaging of membrane protein structure and function

European Biophysics Journal
2021 | Journal article
Contributors: Lazar, Josef; Myskova, Jitka; Bondar, Alexey; Rybakova, Olga; Brynda, Jiri; Khoroshyy, Petro
Source: check_circle
Web of Science Researcher Profile Sync

Optical sensors of heterotrimeric G protein signaling

The FEBS Journal
2021-04 | Journal article
Contributors: Alexey Bondar; Josef Lazar
Source: check_circle
Crossref
grade
Preferred source (of 2)‎

Directionality of Light Absorption in Fluorescent Proteins

Biophysical Journal
2020 | Journal article
Contributors: Lazar, Josef; Rybakova, Olga; Myskova, Jitka; Brynda, Jiri; Khoroshyy, Petro; Martinez-Seara, Hector
Source: check_circle
Web of Science Researcher Profile Sync

Directionality of light absorption and emission in representative fluorescent proteins

Proceedings of the National Academy of Sciences
2020-12-22 | Journal article
Contributors: Jitka Myšková; Olga Rybakova; Jiří Brynda; Petro Khoroshyy; Alexey Bondar; Josef Lazar
Source: check_circle
Crossref
grade
Preferred source (of 2)‎

Quantitative Linear Dichroism Imaging of Molecular Processes in Living Cells Made Simple by Open Software Tools

2020-12-01 | Other
Contributors: Alexey Bondar; Olga Rybakova; Josef Melcr; Jan Dohnálek; Petro Khoroshyy; Ondrej Tichacek; Štěpán Timr; Paul Miclea; Alina Sakhi; Josef Lazar
Source: check_circle
Crossref

The activity of Saccharomyces cerevisiae Na+, K+/H+ antiporter Nha1 is negatively regulated by 14-3-3 protein binding at serine 481

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
2019-12 | Journal article
Part of ISSN: 0167-4889
Source: Self-asserted source
Josef Lazar
grade
Preferred source (of 2)‎

Determinations of Orientation of Membrane-Associated Fluorescent Molecules Made Trivially Simple

Biophysical Journal
2018 | Journal article
Contributors: Lazar, Josef; Rybakova, Olga; Timr, Stepan
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Directionality of Two-Photon Excitation in Representative Fluorescent Proteins

Biophysical Journal
2018 | Journal article
Contributors: Rybakova, Olga; Timr, Stepan; Lazar, Josef
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Web of Science Researcher Profile Sync

Visualization of G-protein-coupled receptors using photoactivated localization microscopy (PALM)

Journal of Bioenergetics and Biomembranes
2018 | Journal article
Contributors: Kozyrina, A.; Ghose, A.; Lazar, J.
Source: check_circle
Web of Science Researcher Profile Sync

Interactions between G Proteins and Non-Stimulated GPCRs Revealed by Two-Photon Polarization Microscopy

Biophysical Journal
2017 | Journal article
Contributors: Bondar, Alexey; Lazar, Josef
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The G protein G(i1) exhibits basal coupling but not preassembly with G protein-coupled receptors

Journal of Biological Chemistry
2017 | Journal article
Contributors: Bondar, Alexey; Lazar, Josef
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Experimental Determination of Single- and Two-Photon Excitation Transition Moments in Representative Fluorescent Proteins

Biophysical Journal
2016 | Journal article
Contributors: Lazar, Josef; Shukla, Prakash; Chazal, Richard; Bondar, Alexey; von Stetten, David; Royant, Antoine
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Precoupling of G Proteins with GPCRs Visualized by Two-Photon Polarization Microscopy

Biophysical Journal
2016 | Journal article
Contributors: Bondar, Alexey; Brzakova, Adela; Timr, Stepan; Lazar, Josef
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Web of Science Researcher Profile Sync

Designing voltage-sensitive probes for neural imaging - Insight from molecular dynamics simulations

European Biophysics Journal
2015 | Journal article
Contributors: Bonhenry, D.; Melcr, J.; Timr, S.; Lazar, J.; Jungwirth, P.
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Web of Science Researcher Profile Sync

Experimental Determination of Transition Dipole Moment Directions in Representative Fluorescent Proteins

Biophysical Journal
2015 | Journal article
Contributors: Kevorkova, Alina; von Stetten, David; Royant, Antoine; Lazar, Josef
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Web of Science Researcher Profile Sync

Nonlinear Optical Properties of Fluorescent Dyes Allow for Accurate Determination of Their Molecular Orientations in Phospholipid Membranes

Journal of Physical Chemistry B
2015 | Journal article
WOSUID:

WOS:000359031400023

Contributors: Timr, Stepan; Brabec, Jiri; Bondar, Alexey; Ryba, Tomas; Zelezny, Milos; Lazar, Josef; Jungwirth, Pavel
Source: Self-asserted source
Josef Lazar via ResearcherID

Accurate Determination of the Orientational Distribution of a Fluorescent Molecule in a Phospholipid Membrane

Journal of Physical Chemistry B
2014 | Journal article
WOSUID:

WOS:000330610400001

Contributors: Timr, S.; Bondar, A.; Cwiklik, L.; Stefl, M.; Hof, M.; Vazdar, M.; Lazar, J.; Jungwirth, P.
Source: Self-asserted source
Josef Lazar via ResearcherID
grade
Preferred source (of 2)‎

Dissociated G alpha(GTP) and G beta gamma Protein Subunits Are the Major Activated Form of Heterotrimeric Gi/o Proteins

Journal of Biological Chemistry
2014 | Journal article
WOSUID:

WOS:000332401700007

Contributors: Bondar, A.; Lazar, J.
Source: Self-asserted source
Josef Lazar via ResearcherID
grade
Preferred source (of 2)‎

Mechanistic Studies of the Genetically Encoded Fluorescent Protein Voltage Probe ArcLight

Plos One
2014 | Journal article
WOSUID:

WOS:000346766900077

Contributors: Han, Zhou; Jin, Lei; Chen, Fuyi; Loturco, Joseph J.; Cohen, Lawrence B.; Bondar, Alexey; Lazar, Josef; Pieribone, Vincent A.
Source: Self-asserted source
Josef Lazar via ResearcherID

Genetically encoded voltage sensors for two-photon polarization microscopy (2PPM)

Society for Neuroscience Abstract Viewer and Itinerary Planner
2013 | Journal article
WOSUID:

BCI:BCI201400184059

Contributors: Bondar, A.; Kuznetsova, V.; Jin, L.; Cohen, L. B.; Pieribone, V. A.; Lazar, J.
Source: Self-asserted source
Josef Lazar via ResearcherID

Modification of Arclight, a Genetically-Encoded Voltage Sensitive Probe: A Study of Mechanism

Biophysical Journal
2013 | Journal article
WOSUID:

WOS:000316074306439

Contributors: Han, Zhou; Jin, Lei; Turavets, Dzmitriy; Cohen, Lawrence B.; Lazar, Josef; Pieribone, Vincent A.
Source: Self-asserted source
Josef Lazar via ResearcherID

Novel Genetically Encoded Probes of G-Protein Activation, Calcium Concentration and Membrane Voltage for Two-Photon Polarization Microscopy

Biophysical Journal
2013 | Journal article
WOSUID:

WOS:000316074306386

Contributors: Lazar, J.; Bondar, A.; Turavets, D.; Kuznetsova, V.
Source: Self-asserted source
Josef Lazar via ResearcherID
grade
Preferred source (of 2)‎

No need to FRET: sensitive imaging of protein structure and function by two-photon polarization microscopy

Biomedical Engineering-Biomedizinische Technik
2012 | Journal article
WOSUID:

BCI:BCI201300166094

Contributors: Lazar, Josef
Source: Self-asserted source
Josef Lazar via ResearcherID
grade
Preferred source (of 2)‎

Novel Genetically Encoded Probes for Observing Molecular Processes in Living Cells by Two-Photon Polarization Microscopy

Biophysical Journal
2012 | Journal article
WOSUID:

WOS:000321561201319

Contributors: Lazar, J.; Bondar, A.
Source: Self-asserted source
Josef Lazar via ResearcherID

Sensitive imaging of cellular processes using two-photon polarization microscopy (2PPM)

Molecular Biology of the Cell
2012 | Journal article
WOSUID:

WOS:000209348601098

Contributors: Bondar, A.; Timr, S.; Lazar, J.
Source: Self-asserted source
Josef Lazar via ResearcherID
grade
Preferred source (of 2)‎

No Need to FRET

Biophysical Journal
2011 | Journal article
WOSUID:

WOS:000306288601135

Contributors: Lazar, J.; Bondar, A.; Firestein, S.
Source: Self-asserted source
Josef Lazar via ResearcherID
grade
Preferred source (of 2)‎

Novel genetically encoded sensors for two-photon polarization microscopy

Society for Neuroscience Abstract Viewer and Itinerary Planner
2011 | Journal article
WOSUID:

BCI:BCI201200080544

Contributors: Lazar, J.; Bondar, A.; Vlckova, V.
Source: Self-asserted source
Josef Lazar via ResearcherID

Two-photon polarization microscopy reveals protein structure and function

Nature Methods
2011 | Journal article
WOSUID:

WOS:000293220600026

Contributors: Lazar, J.; Bondar, A.; Timr, S.; Firestein, S. J.
Source: Self-asserted source
Josef Lazar via ResearcherID

Two-photon polarization microscopy (2PPM) allows sensitive imaging of molecular processes in living cells

Society for Neuroscience Abstract Viewer and Itinerary Planner
2010 | Journal article
WOSUID:

BCI:BCI201100577753

Contributors: Lazar, J.; Bondar, A.; Firestein, S.
Source: Self-asserted source
Josef Lazar via ResearcherID

SRp38 Regulates Alternative Splicing and Is Required for Ca2+ Handling in the Embryonic Heart

Developmental Cell
2009 | Journal article
WOSUID:

WOS:000265470400009

Contributors: Feng, Y.; Valley, M. T.; Lazar, J.; Yang, A. L.; Bronson, R. T.; Firestein, S.; Coetzee, W. A.; Manley, J. L.
Source: Self-asserted source
Josef Lazar via ResearcherID

Method of acquiring structural and functional information on proteins based on polarization fluorescent microscopy and apparatus for making the same

2009-10 | Patent
OTHER-ID:

CZ302233

Contributors: LAZAR JOSEF
Source: Self-asserted source
Josef Lazar via Europe PubMed Central

Prestin as a basis of a voltage sensitive fluorescent protein

Society for Neuroscience Abstract Viewer and Itinerary Planner
2008 | Journal article
WOSUID:

BCI:BCI201200199074

Contributors: Lazar, J.; Bondar, A.; Firestein, S. J.
Source: Self-asserted source
Josef Lazar via ResearcherID

Elephant albumin: A multipurpose pheromone shuttle

Chemistry & Biology
2004 | Journal article
WOSUID:

WOS:000223714900012

Contributors: Lazar, J.; Rasmussen, L. E. L.; Greenwood, D. R.; Bang, I. S.; Prestwich, G. D.
Source: Self-asserted source
Josef Lazar via ResearcherID
grade
Preferred source (of 2)‎

Olfactory adventures of elephantine pheromones

Biochemical Society Transactions
2003 | Journal article
WOSUID:

WOS:000181161000029

Contributors: Rasmussen, L. E. L.; Lazar, J.; Greenwood, D. R.
Source: Self-asserted source
Josef Lazar via ResearcherID
grade
Preferred source (of 2)‎

Molecular and functional characterization of an odorant binding protein of the Asian elephant, Elephas maximus: Implications for the role of lipocalins in mammalian olfaction

Biochemistry
2002 | Journal article
WOSUID:

WOS:000178260400027

Contributors: Lazar, J.; Greenwood, D. R.; Rasmussen, L. E. L.; Prestwich, G. D.
Source: Self-asserted source
Josef Lazar via ResearcherID
grade
Preferred source (of 2)‎

Discrimination of pheromone enantiomers by two pheromone binding proteins from the gypsy moth Lymantria dispar

Biochemistry
2000 | Journal article
WOSUID:

WOS:000088491500030

Contributors: Plettner, E.; Lazar, J.; Prestwich, E. G.; Prestwich, G. D.
Source: Self-asserted source
Josef Lazar via ResearcherID
grade
Preferred source (of 2)‎

ALPHA,ALPHA-DISUBSTITUTED ALLYL SULFONES - AN APPROACH TO THE SYNTHESIS OF VINYL-BRANCHED PHEROMONE ANALOGS

Helvetica Chimica Acta
1994 | Journal article
WOSUID:

WOS:A1994PC18900009

Contributors: Hoskovec, M.; Koutek, B.; Lazar, J.; Streinz, L.; Brozova, E.; Kalinova, B.; Vrkoc, J.
Source: Self-asserted source
Josef Lazar via ResearcherID
grade
Preferred source (of 2)‎

ESTIMATION OF CRITICAL PROPERTIES FROM THE NUMBER OF CARBON-ATOMS IN HOMOLOGOUS SERIES

Collection of Czechoslovak Chemical Communications
1994 | Journal article
WOSUID:

WOS:A1994PC69900003

Contributors: Koutek, B.; Hoskovec, M.; Lazar, J.
Source: Self-asserted source
Josef Lazar via ResearcherID
grade
Preferred source (of 2)‎

Peer review (3 reviews for 2 publications/grants)

Review activity for Journal of cell science. (1)
Review activity for Journal of fluorescence. (2)