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Other IDs

Scopus Author ID: 6506341053
Researcher Name Resolver ID: 1000070452430

Keywords

reproduction, extracellular matrix, lumicrine, male fertility, extracellular signaling, infertility, sperm maturation, epididymis, morphogenesis, organogenesis, gametogenesis, extracellular environment, multicellular system

Countries

Japan

Biography

Various physiological functions equipped in our bodies are possible because cells and tissues interact with each other to construct highly organised organs that are more than just an aggregation of cells. Signaling by extracellular secreted factors plays central roles in this cellular organisation throughout life stages including early embryogenesis, organogenesis, organ function and homeostasis, and gamete production and reproduction.

I have studied the developmental and physiological roles of secretory factors and the extracellular environment in which they function using a variety of approaches such as biochemistry, cell and developmental biology, and genetics including genome editing.

Recently, based on the discovery of lumicrine, a novel secretory signalling system that functions in the lumen of the reproductive tract (Kiyozumi et al., Nat Commun. 2023; Kiyozumi et al., Science. 2020), I have focused on the elucidation of the molecular mechanism of lumicrine, lumicrine-based diagnosis of male infertility and development of male contraception targeting lumicrine signalling.

Activities

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Employment (9)

基礎生物学研究所/National Institute for Basic Biology: 岡崎市/Okazaki, 愛知県/Aichi Prefecture, JP

2024-04-01 to present | 特任研究員/Project Research Staff (生殖細胞研究部門/Division of Germ Cell Biology)
Employment
Source: check_circle
National Institutes of Natural Sciences

National Institute for Basic Biology: Okazaki, JP

2024-04-01 to present
Employment
Source: Self-asserted source
Daiji Kiyozumi

Institute for Protein Research, Osaka University: Suita, Osaka, JP

2023-04-01 to present | Visiting associate professor
Employment
Source: Self-asserted source
Daiji Kiyozumi

Japan Science and Technology Agency: Chiyoda-ku, Tokyo, JP

2021-10-01 to present | Researcher (PRESTO)
Employment
Source: Self-asserted source
Daiji Kiyozumi

Research Institute of Environmental Medicine, Nagoya University: Nagoya, Aichi, JP

2023-04-01 to 2024-03-31 | Designated lecturer
Employment
Source: Self-asserted source
Daiji Kiyozumi

Research Institute for Microbial Diseases, Osaka University: Suita, Osaka, JP

2019-10-01 to 2023-03-31 | Assistant Professor (Department of Experimental Genome Research)
Employment
Source: Self-asserted source
Daiji Kiyozumi

Research Institute for Microbial Diseases, Osaka University: Suita, Osaka, JP

2014-10-01 to 2019-09-30 | Specially Appointed Assistant Professor (Department of Experimental Genome Research)
Employment
Source: Self-asserted source
Daiji Kiyozumi

Institute for Protein Research, Osaka University: Suita, Osaka, JP

2006-04-01 to 2014-09-30 | Researcher (Department of Extracellular Matrix Biochemistry)
Employment
Source: Self-asserted source
Daiji Kiyozumi

Japan Science and Technology Agency: Aichi-gun, Aichi, JP

2001-10-01 to 2006-03-31 | Team Leader (ERATO Sekiguchi Biomatrix Signaling Project)
Employment
Source: Self-asserted source
Daiji Kiyozumi

Education and qualifications (3)

Osaka University Graduate School of Science: Toyanaka, Osaka, JP

1998-04-01 to 2001-09-30 (Department of Biological Science)
Education
Source: Self-asserted source
Daiji Kiyozumi

Osaka University: Toyonaka, Osaka, JP

1996-04-01 to 1998-03-31 (Departmemt of Chemistry)
Education
Source: Self-asserted source
Daiji Kiyozumi

Osaka University: Toyonaka, Osaka, JP

1992-04-01 to 1996-03-31 (Department of Chemistry)
Education
Source: Self-asserted source
Daiji Kiyozumi

Funding (11)

Molecular mechanisms of sperm maturation by reproductive tract 生殖路による精子成熟の分子機構解明

2023-04 to 2025-03 | Grant
Chugai Foundation for Innovative Drug Discovery Science (Chuo-ku, JP)
Source: Self-asserted source
Daiji Kiyozumi

Development of Diagnostic Methods for Male Infertility by Functional Evaluation of Sperm Surface Molecules

2022-04 to 2023-03 | Grant
The Futaba Foundation (Mobara, Chiba, JP)
Source: Self-asserted source
Daiji Kiyozumi

Mechanism of sperm maturation through proteolysis

2022-04 to 2023-03 | Grant
Japan Foundation for Applied Enzymology (Chuo-ku, Osaka, JP)
Source: Self-asserted source
Daiji Kiyozumi

Regulatory Mechanism of Epithelial Integrity and Function by Lumicrine Signaling

2021-10 to 2025-03 | Grant
Japan Science and Technology Agency (Chiyoda-ku, Tokyo, JP)
Source: Self-asserted source
Daiji Kiyozumi

新規因子探索による分泌シグナル伝達ルミクリンの包括的解析

2021-07-09 to 2023-03-31 | Grant
Japan Society for the Promotion of Science (Tokyo, JP)
URL: https://app.dimensions.ai/details/grant/grant.9745611
GRANT_NUMBER: 21K19263
Source: Self-asserted source
Daiji Kiyozumi via DimensionsWizard

新規分泌シグナル伝達ルミクリンの分子機構解明

2021-04-01 to 2024-03-31 | Grant
Japan Society for the Promotion of Science (Tokyo, JP)
URL: https://app.dimensions.ai/details/grant/grant.9672601
GRANT_NUMBER: 21H02487
Source: Self-asserted source
Daiji Kiyozumi via DimensionsWizard

精子受精能を制御する生殖路管腔環境のデザイン原理

2021-04-01 to 2023-03-31 | Grant
Japan Society for the Promotion of Science (Tokyo, JP)
URL: https://app.dimensions.ai/details/grant/grant.9670383
GRANT_NUMBER: 21H00231
Source: Self-asserted source
Daiji Kiyozumi via DimensionsWizard

Analysis of extracellular matrices that regulate mammalian reproduction

2015-04-01 to 2018-03-31 | Grant
Japan Society for the Promotion of Science (Tokyo, JP)
URL: https://app.dimensions.ai/details/grant/grant.5871140
GRANT_NUMBER: 15K06999
Source: Self-asserted source
Daiji Kiyozumi via DimensionsWizard

Physiological roles of basement membranes in sea urchin embryogenesis

2014-04 to 2015-03 | Grant
Japan Association for Marine Biology (Shimoda, JP)
Source: Self-asserted source
Daiji Kiyozumi

Physiological roles of basement membranes in sea urchin embryogenesis

2013-04 to 2014-03 | Grant
Japan Association for Marine Biology (Shimoda, JP)
Source: Self-asserted source
Daiji Kiyozumi

Customization and cellular recognition of the extracellular matrix

2005-01-01 to 2009-12-31 | Grant
Japan Society for the Promotion of Science (Tokyo, JP)
URL: https://app.dimensions.ai/details/grant/grant.5931837
GRANT_NUMBER: 17082005
Source: Self-asserted source
Daiji Kiyozumi via DimensionsWizard

Works (33)

CRISPR/Cas9‐mediated genome editing reveals seven testis‐enriched transmembrane glycoproteins dispensable for male fertility in mice

Andrology
2023-12-12 | Journal article
DOI: 10.1111/andr.13564
Contributors: Yo Ogawa; Yonggang Lu; Daiji Kiyozumi; Hsin‐Yi Chang; Masahito Ikawa
Source: check_circle
Crossref

Lumicrine signaling: Extracellular regulation of sperm maturation in the male reproductive tract lumen

Genes to Cells
2023-11 | Journal article
DOI: 10.1111/gtc.13066
Contributors: Daiji Kiyozumi
Source: check_circle
Crossref

New insights into lumicrine secreted signalling that regulates the function of the mammalian male reproductive tract

Developmental Biology
2023-11 | Journal article
DOI: 10.1016/j.ydbio.2023.08.005
Contributors: Daiji Kiyozumi
Source: check_circle
Crossref

ADGRG2 is dispensable for lumicrine signalling regulating epididymal initial segment differentiation and gene expression

2023-08-01 | Other
DOI: 10.1093/biolre/ioad087
Contributors: Daiji Kiyozumi; Masahito Ikawa
Source: check_circle
Crossref

A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility

Nature Communications
2023-04-24 | Journal article
DOI: 10.1038/s41467-023-37984-x
Contributors: Daiji Kiyozumi; Kentaro Shimada; Michael Chalick; Chihiro Emori; Mayo Kodani; Seiya Oura; Taichi Noda; Tsutomu Endo; Martin M. Matzuk; Daniel H. Wreschner et al.
Source: check_circle
Crossref

The molecular mechanisms of mammalian sperm maturation regulated by NELL2-ROS1 lumicrine signaling.

Journal of biochemistry
2022-12-01 | Journal article
DOI: 10.1093/jb/mvac071
PMID: 36071564
Contributors: Daiji Kiyozumi; Kiyozumi D
Source: Self-asserted source
Daiji Kiyozumi

Proteolysis in Reproduction: Lessons From Gene-Modified Organism Studies.

Frontiers in endocrinology
2022-05-04 | Journal article
DOI: 10.3389/fendo.2022.876370
PMID: 35600599
PMC: PMC9114714
Contributors: Daiji Kiyozumi; Kiyozumi D; Ikawa M
Source: Self-asserted source
Daiji Kiyozumi

Human disease-associated extracellular matrix orthologs ECM3 and QBRICK regulate primary mesenchymal cell migration in sea urchin embryos.

Experimental animals
2021-04-06 | Journal article
DOI: 10.1538/expanim.21-0001
PMID: 33828019
Source: Self-asserted source
Daiji Kiyozumi

NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility.

Science (New York, N.Y.)
2020-06-01 | Journal article
DOI: 10.1126/science.aay5134
PMID: 32499443
PMC: PMC7396227
Source: Self-asserted source
Daiji Kiyozumi
grade
Preferred source (of 2)‎

CRISPR/Cas9-Mediated Genome Editing Reveals <i>Oosp</i> Family Genes are Dispensable for Female Fertility in Mice.

Cells
2020-03-28 | Journal article
DOI: 10.3390/cells9040821
PMID: 32231122
PMC: PMC7226750
Source: Self-asserted source
Daiji Kiyozumi
grade
Preferred source (of 2)‎

Laminin is the ECM niche for trophoblast stem cells.

Life science alliance
2020-01-14 | Journal article
DOI: 10.26508/lsa.201900515
PMID: 31937556
PMC: PMC6977391
Source: Self-asserted source
Daiji Kiyozumi
grade
Preferred source (of 2)‎

Genetic mutation of Frem3 does not causeFraser syndrome in mice.

Experimental animals
2019-09-26 | Journal article
DOI: 10.1538/expanim.19-0088
PMID: 31554749
PMC: PMC7004800
Source: Self-asserted source
Daiji Kiyozumi

CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice†.

Biology of reproduction
2019-08-01 | Journal article
DOI: 10.1093/biolre/ioz103
PMID: 31201419
PMC: PMC6735960
Source: Self-asserted source
Daiji Kiyozumi

Lvrn expression is not critical for mouse placentation.

The Journal of reproduction and development
2019-02-10 | Journal article
DOI: 10.1262/jrd.2018-157
PMID: 30745494
PMC: PMC6584185
Source: Self-asserted source
Daiji Kiyozumi

Ventricular-subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche.

Molecular biology of the cell
2018-10-31 | Journal article
DOI: 10.1091/mbc.e18-05-0286
PMID: 30379609
PMC: PMC6337917
Source: Self-asserted source
Daiji Kiyozumi

Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality.

Life science alliance
2018-09-10 | Journal article
DOI: 10.26508/lsa.201800064
PMID: 30456378
PMC: PMC6238537
Source: Self-asserted source
Daiji Kiyozumi
grade
Preferred source (of 2)‎

Placenta-specific gene manipulation using lentiviral vector and its application

Placenta
2017 | Journal article
DOI: 10.1016/j.placenta.2017.09.012
EID:

2-s2.0-85030630228
Contributors: Tobita, T.; Kiyozumi, D.; Ikawa, M.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice

Proceedings of the National Academy of Sciences of the United States of America
2016 | Journal article
DOI: 10.1073/pnas.1608458113
EID:

2-s2.0-84978040313
Contributors: Miyata, H.; Castaneda, J.M.; Fujihara, Y.; Yu, Z.; Archambeault, D.R.; Isotani, A.; Kiyozumi, D.; Kriseman, M.L.; Mashiko, D.; Matsumura, T. et al.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Lentiviral vector-mediated complementation restored fetal viability but not placental hyperplasia in Plac1-deficient mice

Biology of Reproduction
2016 | Journal article
DOI: 10.1095/biolreprod.115.133454
EID:

2-s2.0-84957928374
Contributors: Muto, M.; Fujihara, Y.; Tobita, T.; Kiyozumi, D.; Ikawa, M.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

In situ detection of integrin ligands

Current Protocols in Cell Biology
2014 | Journal article
DOI: 10.1002/0471143030.cb0907s65
EID:

2-s2.0-84949293437
Contributors: Kiyozumi, D.; Sato-Nishiuchi, R.; Sekiguchi, K.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

GPR56 Functions Together with α3β1 Integrin in Regulating Cerebral Cortical Development

PLoS ONE
2013 | Journal article
DOI: 10.1371/journal.pone.0068781
EID:

2-s2.0-84879913389
Contributors: Jeong, S.-J.; Luo, R.; Singer, K.; Giera, S.; Kreidberg, J.; Kiyozumi, D.; Shimono, C.; Sekiguchi, K.; Piao, X.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Basement membrane assembly of the integrin α8β1 ligand nephronectin requires Fraser syndrome-associated proteins

Journal of Cell Biology
2012 | Journal article
DOI: 10.1083/jcb.201203065
EID:

2-s2.0-84862633622
Contributors: Kiyozumi, D.; Takeichi, M.; Nakano, I.; Sato, Y.; Fukuda, T.; Sekiguchi, K.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Polydom/SVEP1 is a ligand for integrin α9β1

Journal of Biological Chemistry
2012 | Journal article
DOI: 10.1074/jbc.M112.355016
EID:

2-s2.0-84864082579
Contributors: Sato-Nishiuchi, R.; Nakano, I.; Ozawa, A.; Sato, Y.; Takeichi, M.; Kiyozumi, D.; Yamazaki, K.; Yasunaga, T.; Futaki, S.; Sekiguchi, K.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Fused pulmonary lobes is a rat model of human Fraser syndrome

Biochemical and Biophysical Research Communications
2011 | Journal article
DOI: 10.1016/j.bbrc.2011.06.174
EID:

2-s2.0-79960835972
Contributors: Kiyozumi, D.; Nakano, I.; Takahashi, K.L.; Hojo, H.; Aoyama, H.; Sekiguchi, K.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Heterozygous mutations of FREM1 are associated with an increased risk of isolated metopic craniosynostosis in humans and mice

PLoS Genetics
2011 | Journal article
DOI: 10.1371/journal.pgen.1002278
EID:

2-s2.0-80053437424
Contributors: Vissers, L.E.L.M.; Cox, T.C.; Maga, A.M.; Short, K.M.; Wiradjaja, F.; Janssen, I.M.; Jehee, F.; Bertola, D.; Liu, J.; Yagnik, G. et al.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Identification of genes expressed during hair follicle induction

Journal of Dermatology
2011 | Journal article
DOI: 10.1111/j.1346-8138.2010.01050.x
EID:

2-s2.0-79959448073
Contributors: Kiyozumi, D.; Osada, A.; Sugimoto, N.; Weber, C.N.; Ono, Y.; Imai, T.; Okada, A.; Sekiguchi, K.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Genetic analysis of fin development in zebrafish identifies furin and Hemicentin1 as potential novel fraser syndrome disease genes

PLoS Genetics
2010 | Journal article
DOI: 10.1371/journal.pgen.1000907
EID:

2-s2.0-77952354471
Contributors: Carney, T.J.; Feitosa, N.M.; Sonntag, C.; Slanchev, K.; Kluger, J.; Kiyozumi, D.; Gebauer, J.M.; Talbot, J.C.; Kimmel, C.B.; Sekiguchi, K. et al.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Transcriptome-based systematic identification of extracellular matrix proteins

Proceedings of the National Academy of Sciences of the United States of America
2008 | Journal article
DOI: 10.1073/pnas.0803640105
EID:

2-s2.0-51349089252
Contributors: Manabe, R.-I.; Tsutsui, K.; Yamada, T.; Kimura, M.; Nakano, I.; Shimono, C.; Sanzen, N.; Furutani, Y.; Fukuda, T.; Oguri, Y. et al.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Frem3, a member of the 12 CSPG repeats-containing extracellular matrix protein family, is a basement membrane protein with tissue distribution patterns distinct from those of Fras1, Frem2, and QBRICK/Frem1

Matrix Biology
2007 | Journal article
DOI: 10.1016/j.matbio.2007.03.001
EID:

2-s2.0-34447545502
Contributors: Kiyozumi, D.; Sugimoto, N.; Nakano, I.; Sekiguchi, K.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Breakdown of the reciprocal stabilization of QBRICK/Frem1, Fras1, and Frem2 at the basement membrane provokes Fraser syndrome-like defects

Proceedings of the National Academy of Sciences of the United States of America
2006 | Journal article
DOI: 10.1073/pnas.0601011103
EID:

2-s2.0-33747051471
Contributors: Kiyozumi, D.; Sugimoto, N.; Sekiguchi, K.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Expression of MAEG, a novel basement membrane protein, in mouse hair follicle morphogenesis

Experimental Cell Research
2005 | Journal article
DOI: 10.1016/j.yexcr.2004.04.053
EID:

2-s2.0-9644300843
Contributors: Osada, A.; Kiyozumi, D.; Tsutsui, K.; Ono, Y.; Weber, C.N.; Sugimoto, N.; Imai, T.; Okada, A.; Sekiguchi, K.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Identification of a novel cell-adhesive protein spatiotemporally expressed in the basement membrane of mouse developing hair follicle

Experimental Cell Research
2005 | Journal article
DOI: 10.1016/j.yexcr.2005.01.020
EID:

2-s2.0-18844448193
Contributors: Kiyozumi, D.; Osada, A.; Sugimoto, N.; Weber, C.N.; Ono, Y.; Imai, T.; Okada, A.; Sekiguchi, K.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Pollen UDP-glucose pyrophosphorylase showing polymorphism well-correlated to the S genotype of Pyrus pyrifolia

Sexual Plant Reproduction
2002 | Journal article
DOI: 10.1007/s00497-001-0125-1
EID:

2-s2.0-0035733683
Contributors: Kiyozumi, D.; Ishimizu, T.; Nakanishi, T.; Norioka, S.
Source: Self-asserted source
Daiji Kiyozumi via Scopus - Elsevier

Peer review (1 review for 1 publication/grant)

Review activity for Nature chemical biology. (1)
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