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Works (43)

Stress-Inducible Expression of HvABF2 Transcription Factor Improves Water Deficit Tolerance in Transgenic Barley Plants

Plants
2024-11-05 | Journal article
DOI: 10.3390/plants13223113
Contributors: Rabea Al-Sayaydeh; Jamal Ayad; Wendy Harwood; Ayed M. Al-Abdallat
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Breaking transformation barriers

Nature Plants
2023-05-16 | Journal article
DOI: 10.1038/s41477-023-01420-1
Contributors: Wendy Harwood
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Development of a highly efficient LbCas12a CRISPR system in barley with additional utility in Brassica oleracea

2022-09-19 | Preprint
DOI: 10.21203/rs.3.rs-2049530/v1
Contributors: Tom Lawrenson; Alison Hinchliffe; Macarena Forner; Wendy Harwood
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Reconstitution of monoterpene indole alkaloid biosynthesis in genome engineered Nicotiana benthamiana

Communications Biology
2022-09-10 | Journal article
DOI: 10.1038/s42003-022-03904-w
Contributors: Quentin M. Dudley; Seohyun Jo; Delia Ayled Serna Guerrero; Monika Chhetry; Mark A. Smedley; Wendy A. Harwood; Nathaniel H. Sherden; Sarah E. O’Connor; Lorenzo Caputi; Nicola J. Patron
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Regulation of Amylose Content by Single Mutations at an Active Site in the Wx-B1 Gene in a Tetraploid Wheat Mutant

International Journal of Molecular Sciences
2022-07-29 | Journal article
DOI: 10.3390/ijms23158432
Contributors: Yulong Li; Hassan Karim; Bang Wang; Carlos Guzmán; Wendy Harwood; Qiang Xu; Yazhou Zhang; Huaping Tang; Yunfeng Jiang; Pengfei Qi et al.
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Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62

Nature Communications
2022-03-25 | Journal article
DOI: 10.1038/s41467-022-29132-8
Contributors: Guotai Yu; Oadi Matny; Nicolas Champouret; Burkhard Steuernagel; Matthew J. Moscou; Inmaculada Hernández-Pinzón; Phon Green; Sadiye Hayta; Mark Smedley; Wendy Harwood et al.
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Reconstitution of monoterpene indole alkaloid biosynthesis in genome engineered Nicotiana benthamiana

2021-08-12 | Preprint
DOI: 10.1101/2021.08.12.456143
Contributors: Quentin M. Dudley; Seohyun Jo; Delia Ayled Serna Guerrero; Monika Chhetry; Mark A. Smedley; Wendy A. Harwood; Nathaniel H. Sherden; Sarah E. O’Connor; Lorenzo Caputi; Nicola J. Patron
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Crossref

In-planta Gene Targeting in Barley using Cas9, with and without Geminiviral Replicons

2021-02-04 | Preprint
DOI: 10.1101/2021.02.04.429787
Contributors: Tom Lawrenson; Alison Hinchliffe; Martha Clarke; Yvie Morgan; Wendy Harwood
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Overexpression of the High-Affinity Nitrate Transporter OsNRT2.3b Driven by Different Promoters in Barley Improves Yield and Nutrient Uptake Balance

International Journal of Molecular Sciences
2020-02-15 | Journal article
DOI: 10.3390/ijms21041320
Contributors: Bingbing Luo; Man Xu; Limei Zhao; Peng Xie; Yi Chen; Wendy Harwood; Guohua Xu; Xiaorong Fan; Anthony J. Miller
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TRA1: a locus responsible for controllingAgrobacterium-mediated transformability in barley

2019-12-19 | Preprint
DOI: 10.1101/2019.12.19.882274
Contributors: Beata Orman-Ligeza; Wendy Harwood; Pete E. Hedley; Alison Hinchcliffe; Malcolm Macaulay; Cristobal Uauy; Kay Trafford
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Speed breeding in growth chambers and glasshouses for crop breeding and model plant research

2018-07 | Journal article
DOI: 10.1101/369512
OTHER-ID:

PPR45214
Contributors: Ghosh S; Watson A; Gonzalez-Navarro OE; Ramirez-Gonzalez RH; Yanes L; Mendoza-Suárez M; Simmonds J; Wells R; Rayner T; Green P et al.
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

The wheatSr22,Sr33,Sr35andSr45genes confer resistance against stem rust in barley

2018-07 | Journal article
DOI: 10.1101/374637
OTHER-ID:

PPR45618
Contributors: Md. Hatta MA; Johnson R; Matny O; Smedley MA; Yu G; Chakraborty S; Bhatt D; Xia X; Arora S; Steuernagel B et al.
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

A Welcome Proposal to Amend the GMO Legislation of the EU.

Trends in biotechnology
2018-05 | Journal article
DOI: 10.1016/j.tibtech.2018.05.001
PMID: 29807731
Contributors: Eriksson D; Harwood W; Hofvander P; Jones H; Rogowsky P; Stöger E; Visser RGF
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Magnesium Increases Homoeologous Crossover Frequency During Meiosis in ZIP4 (Ph1 Gene) Mutant Wheat-Wild Relative Hybrids.

Frontiers in Plant Science
2018-04 | Journal article
DOI: 10.3389/fpls.2018.00509
PMID: 29731763
PMC: PMC5920029
Contributors: Rey MD; Martín AC; Smedley M; Hayta S; Harwood W; Shaw P; Moore G
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Magnesium increases homoeologous crossover frequency in ZIP4 ( Ph1 ) mutant wheat-wild relative hybrids

2018-03 | Journal article
DOI: 10.1101/278341
OTHER-ID:

PPR11713
Contributors: Rey M; Martin AC; Smedley M; Hayta S; Harwood W; Shaw P; Moore G
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Speed breeding is a powerful tool to accelerate crop research and breeding.

Nature plants
2018-01 | Journal article
DOI: 10.1038/s41477-017-0083-8
PMID: 29292376
Contributors: Watson A; Ghosh S; Williams MJ; Cuddy WS; Simmonds J; Rey MD; Asyraf Md Hatta M; Hinchliffe A; Steed A; Reynolds D et al.
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Speed breeding: a powerful tool to accelerate crop research and breeding

2017-07 | Journal article
DOI: 10.1101/161182
OTHER-ID:

PPR16598
Contributors: Watson A; Ghosh S; Williams M; Cuddy WS; Simmonds J; Rey M; Hatta MAM; Hinchliffe A; Steed A; Reynolds D et al.
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease.

Genome biology
2015-11 | Journal article
DOI: 10.1186/s13059-015-0826-7
PMID: 26616834
PMC: PMC4663725
Contributors: Lawrenson T; Shorinola O; Stacey N; Li C; Østergaard L; Patron N; Uauy C; Harwood W
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Challenges facing European agriculture and possible biotechnological solutions.

Critical reviews in biotechnology
2015-07 | Journal article
DOI: 10.3109/07388551.2015.1055707
PMID: 26133365
Contributors: Ricroch A; Harwood W; Svobodová Z; Sági L; Hundleby P; Badea EM; Rosca I; Cruz G; Salema Fevereiro MP; Marfà Riera V et al.
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Gateway®-compatible plant transformation vectors.

Methods in molecular biology (Clifton, N.J.)
2015-01 | Journal article
DOI: 10.1007/978-1-4939-1695-5_1
PMID: 25300827
Contributors: Smedley MA; Harwood WA
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Wheat (Triticum aestivum L.) transformation using mature embryos.

Methods in molecular biology (Clifton, N.J.)
2015-01 | Journal article
DOI: 10.1007/978-1-4939-1695-5_16
PMID: 25300842
Contributors: Medvecká E; Harwood WA
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Overexpression of the transcription factor HvSNAC1 improves drought tolerance in barley (Hordeum vulgare L.)

Molecular breeding.
2014-02 | Journal article
DOI: 10.1007/s11032-013-9958-1
OTHER-ID:

IND500722712
Contributors: Al Abdallat AM; Ayad JY; Abu Elenein JM; Al Ajlouni Z; Harwood WA
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

A protocol for high-throughput Agrobacterium-mediated barley transformation.

Methods in molecular biology (Clifton, N.J.)
2014-01 | Journal article
DOI: 10.1007/978-1-62703-715-0_20
PMID: 24243209
Contributors: Harwood WA
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Analysis of T-DNA/Host-Plant DNA Junction Sequences in Single-Copy Transgenic Barley Lines.

Biology
2014-01 | Journal article
DOI: 10.3390/biology3010039
PMID: 24833334
PMC: PMC4009757
Contributors: Bartlett JG; Smedley MA; Harwood WA
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Overexpression of cytokinin dehydrogenase genes in barley (Hordeum vulgare cv. Golden Promise) fundamentally affects morphology and fertility.

PloS one
2013-11 | Journal article
DOI: 10.1371/journal.pone.0079029
PMID: 24260147
PMC: PMC3829838
Contributors: Mrízová K; Jiskrová E; Vyroubalová Š; Novák O; Ohnoutková L; Pospíšilová H; Frébort I; Harwood WA; Galuszka P
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Transgenic barley lines prove the involvement of TaCBF14 and TaCBF15 in the cold acclimation process and in frost tolerance.

Journal of experimental botany
2013-04 | Journal article
DOI: 10.1093/jxb/ert050
PMID: 23567863
PMC: PMC3638819
Contributors: Soltész A; Smedley M; Vashegyi I; Galiba G; Harwood W; Vágújfalvi A
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Electrophoretic and chromatographic evaluation of transgenic barley expressing a bacterial dihydrodipicolinate synthase.

Electrophoresis
2012-08 | Journal article
DOI: 10.1002/elps.201200033
PMID: 22887157
Contributors: Ohnoutkova L; Zitka O; Mrizova K; Vaskova J; Galuszka P; Cernei N; Smedley MA; Harwood WA; Adam V; Kizek R
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Genetic and physiological analysis of Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids.

Journal of experimental botany
2012-07 | Journal article
DOI: 10.1093/jxb/ers138
PMID: 22791821
PMC: PMC3421992
Contributors: Gasperini D; Greenland A; Hedden P; Dreos R; Harwood W; Griffiths S
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Advances and remaining challenges in the transformation of barley and wheat.

Journal of experimental botany
2011-12 | Journal article
DOI: 10.1093/jxb/err380
PMID: 22140237
Contributors: Harwood WA
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Comparison of DNA walking methods for isolation of transgene-flanking regions in GM potato.

Molecular biotechnology
2011-09 | Journal article
DOI: 10.1007/s12033-010-9371-5
PMID: 21225379
Contributors: Cullen D; Harwood W; Smedley M; Davies H; Taylor M
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

The role of alpha-glucosidase in germinating barley grains.

Plant physiology
2010-11 | Journal article
DOI: 10.1104/pp.110.168328
PMID: 21098673
Contributors: Stanley D; Rejzek M; Naested H; Smedley M; Otero S; Fahy B; Thorpe F; Nash RJ; Harwood W; Svensson B et al.
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Intron-mediated enhancement as a method for increasing transgene expression levels in barley.

Plant biotechnology journal
2009-09 | Journal article
DOI: 10.1111/j.1467-7652.2009.00448.x
PMID: 19781005
Contributors: Bartlett JG; Snape JW; Harwood WA
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Barley transformation using Agrobacterium-mediated techniques.

Methods in molecular biology (Clifton, N.J.)
2009-01 | Journal article
DOI: 10.1007/978-1-59745-379-0_9
PMID: 19009444
Contributors: Harwood WA; Bartlett JG; Alves SC; Perry M; Smedley MA; Leyland N; Snape JW
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Barley transformation using biolistic techniques.

Methods in molecular biology (Clifton, N.J.)
2009-01 | Journal article
DOI: 10.1007/978-1-59745-379-0_8
PMID: 19009443
Contributors: Harwood WA; Smedley MA
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

High-throughput Agrobacterium-mediated barley transformation.

Plant methods
2008-09 | Journal article
DOI: 10.1186/1746-4811-4-22
PMID: 18822125
PMC: PMC2562381
Contributors: Bartlett JG; Alves SC; Smedley M; Snape JW; Harwood WA
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Fluorescence in situ hybridization to localize transgenes in plant chromosomes.

Methods in molecular biology (Clifton, N.J.)
2005-01 | Journal article
PMID: 15310931
Contributors: Harwood WA; Bilham LJ; Travella S; Salvo-Garrido H; Snape JW
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques.

Plant cell reports
2004-11 | Journal article
DOI: 10.1007/s00299-004-0892-x
PMID: 15761662
Contributors: Travella S; Ross SM; Harden J; Everett C; Snape JW; Harwood WA
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Additional introns inserted within the luciferase reporter gene stabilise transgene expression in wheat.

Plant science.
2004-11 | Journal article
DOI: 10.1016/j.plantsci.2004.06.009
OTHER-ID:

IND43645592
Contributors: Bourdon V; Wickham A; Lonsdale D; Harwood W
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

The distribution of transgene insertion sites in barley determined by physical and genetic mapping.

Genetics
2004-07 | Journal article
DOI: 10.1534/genetics.103.023747
PMID: 15280249
PMC: PMC1470960
Contributors: Salvo-Garrido H; Travella S; Bilham LJ; Harwood WA; Snape JW
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

An efficient method for the physical mapping of transgenes in barley using in situ hybridization.

Genome
2001-02 | Journal article
DOI: 10.1139/gen-44-1-104
PMID: 11269343
Contributors: Salvo-Garrido H; Travella S; Schwarzacher T; Harwood WA; Snape JW
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

The effect of DNA/gold particle preparation technique, and particle bombardment device, on the transformation of barley (Hordeum vulgare).

Euphytica.
2000-01 | Journal article
DOI: 10.1023/a:1003700300235
OTHER-ID:

IND22046483
Contributors: Harwood WA; Ross SM; Cilento P; Snape JW
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

An analysis of seed development in Pisum sativum. XIII. The chemical induction of storage product mutants.

Plant breeding.
1990-12 | Journal article
DOI: 10.1111/j.1439-0523.1990.tb01290.x
OTHER-ID:

IND93013967
Contributors: Wang TL; Hadavizideh A; Harwood A; Welham TJ; Harwood WA; Faulks R; Hedley CL
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central

Protoplast microinjection using agarose microdrops.

Methods in molecular biology (Clifton, N.J.)
1990-01 | Journal article
DOI: 10.1385/0-89603-161-6:323
PMID: 21390618
Contributors: Harwood WA; Davies DR
Source: Self-asserted source
Wendy Harwood via Europe PubMed Central
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