Lenka Hernychova (0000-0002-4352-0626)

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I deal with solving different scientific projects, leading the proteomic group, supervising students, and teaching students at universities (the University of Pardubice, Faculty of Pharmacy in Hradec Kralove, Faculty of Military Health Sciences, the University of Defence in Hradec Králové, and the University of Palacky in Olomouc).
Concerning my research experience, it includes mainly analyses of biological materials by mass spectrometry. I work with bacterial cells (e.g. study of virulence factors), cell lines (detection of the quantitative changes in their proteomes after internal or external treatments), or human clinical materials like a serum, tissues, and ascitic fluids (identification of proposed cancer protein markers). I am also able to detect proteins from different cell compartments (membrane or nuclear proteins) or secreted immunodominant, and post-translationally modified proteins. Last 5 years, I am highly interested in the detection of changes in the protein structure after interaction with ligands (small molecules, proteins, or nucleic acids). For this purpose, I use an advanced hydrogen/deuterium exchange technique connected with high-resolution mass spectrometry. This type of study of protein dynamics is provided only by two institutions in the Czech Republic.

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Masaryk University: Brno, CZ

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Lenka Hernychova

https://orcid.org/0000-0002-4352-0626

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Employment (2)

Masaryk University: Brno, CZ

Masaryk Memorial Cancer Institute: Brno, CZ

Education and qualifications (2)

Masaryk Memorial Cancer Institute: Brno, CZ

University of Pardubice: Pardubice, CZ

Funding (1)

22-09853S

Works (50 of 83)

Monitoring Changes in the Levels of Newly Synthesized Proteins in Response to Nutlin‐3 Treatment

Direct activation of HSF1 by macromolecular crowding and misfolded proteins

Metaproteomic analysis from cervical biopsies and cytologies identifies proteinaceous biomarkers representing both human and microbial species

Multi-Omic Analysis of Esophageal Adenocarcinoma Uncovers Candidate Therapeutic Targets and Cancer-Selective Posttranscriptional Regulation

Biochemical evidence for conformational variants in the anti-viral and pro-metastatic protein IFITM1

N-glycan profiling of tissue samples to aid breast cancer subtyping

Study of Protein Conformational Dynamics Using Hydrogen/Deuterium Exchange Mass Spectrometry

Identification, characterization, and engineering of glycosylation in thrombolytics

Inherited CDA-I disease: anemia-associated mutations disrupt CDIN1-Codanin1 complex

The risk of second primary malignancies in colorectal cancer patients using calcium channel blockers

Next-generation sequencing of a combinatorial peptide phage library screened against ubiquitin identifies peptide aptamers that can inhibit the in vitro ubiquitin transfer cascade

Emergent Role of IFITM1/3 towards Splicing Factor (SRSF1) and Antigen-Presenting Molecule (HLA-B) in Cervical Cancer

Cryptic <i>in vitro</i> ubiquitin ligase activity of HDMX towards p53 is probably regulated by an induced fit mechanism

Changes of serum protein N-glycosylation in cancer

CHIP-dependent regulation of the actin cytoskeleton is linked to neuronal cell membrane integrity

Engineering the protein dynamics of an ancestral luciferase

N-Glycome changes reflecting resistance to platinum-based chemotherapy in ovarian cancer

Hydrogen deuterium exchange mass spectrometry identifies the dominant paratope in CD20 antigen binding to the NCD1.2 monoclonal antibody

The MDM2 ligand Nutlin-3 differentially alters expression of the immune blockade receptors PD-L1 and CD276

Allosteric changes in HDM2 by the ATM phosphomimetic S395D mutation: implications on HDM2 function

Glycoproteins in the Sera of Oncological Patients

The effects of IFITM1 and IFITM3 gene deletion on IFNγ stimulated protein synthesis

Light-Emitting Dehalogenases: Reconstruction of Multifunctional Biocatalysts

An inter-subunit protein-peptide interface that stabilizes the specific activity and oligomerization of the AAA+ chaperone Reptin

Importance of Membrane Proteins in the Treatment of Tumor Diseases and the Possibilities of Their Further Study

Proteogenomic Platform for Identification of Tumor Specific Antigens

Microgradient separation technique for purification and fractionation of permethylated N-glycans before mass spectrometric analyses

The Sequence-specific Peptide-binding Activity of the Protein Sulfide Isomerase AGR2 Directs Its Stable Binding to the Oncogenic Receptor EpCAM

Electrochemistry and electron paramagnetic resonance spectroscopy of cytochrome c and its heme-disrupted analogs

Na + /K + -ATPase interaction with methylglyoxal as reactive metabolic side product

ΔNp63 activates EGFR signaling to induce loss of adhesion in triple-negative basal-like breast cancer cells

Novel Entropically Driven Conformation-specific Interactions with Tomm34 Protein Modulate Hsp70 Protein Folding and ATPase Activities

Discovery of a novel ligand that modulates the protein–protein interactions of the AAA+ superfamily oncoprotein reptin

Protein–Protein Interactions Modulate the Docking-Dependent E3-Ubiquitin Ligase Activity of Carboxy-Terminus of Hsc70-Interacting Protein (CHIP)*

Adenoviral Vectors in Gene Therapy

What Can Study of Oligomerization of Proteins in the Process of Oncogenesis Bring Us?

‘Sugars Interfere’ or Glycomics in the Field of Cancer Biomarkers

Investigation of protein FTT1103 electroactivity using carbon and mercury electrodes. Surface-inhibition approach for disulfide oxidoreductases using silver amalgam powder

Analysis of Phosphoproteins and Signalling Pathways by Quantitative Proteomics

Analysis of Protein Using Mass Spectrometry

New Trends in the Study of Protein Glycosylation in Oncological Diseases

Quantitative Mass Spectrometry and Its Utilization in Oncology

Quantitative Proteomic Profiling of Pleomorphic Human Sarcoma Identifies CLIC1 as a Dominant Pro-Oncogenic Receptor Expressed in Diverse Sarcoma Types

The Assembly and Intermolecular Properties of the Hsp70-Tomm34-Hsp90 Molecular Chaperone Complex

Identification of a second Nutlin-3 responsive interaction site in the N-terminal domain of MDM2 using hydrogen/deuterium exchange mass spectrometry

Use of magnetic hydrazide-modified polymer microspheres for enrichment of Francisella tularensis glycoproteins

Characterization of Protein Glycosylation in Francisella tularensis subsp. holarctica

The new platinum-based anticancer agent LA-12 induces retinol binding protein 4 in vivo

Phosphoproteomics: Searching for a needle in a haystack

A Multiprotein Binding Interface in an Intrinsically Disordered Region of the Tumor Suppressor Protein Interferon Regulatory Factor-1