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Scopus Author ID: 36058900100

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Employment (1)

University of Warwick: Coventry, GB

Professor of Microbial Biophysics (School of Life Sciences)
Employment
Source: Self-asserted source
Séamus Holden

Funding (1)

Super-resolving the physical mechanisms of bacterial cell division

2022-06-01 to 2028-06-01 | Grant
Wellcome Trust (London, UK, GB)
GRANT_NUMBER:

206670/A/17/Z

Part of GRANT_NUMBER:

206670/Z/17/Z
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WT Grant Tracker

Works (41)

Self-organization of mortal filaments and its role in bacterial division ring formation

Nature Physics
2024-10 | Journal article
DOI: 10.1038/s41567-024-02597-8
Contributors: Christian Vanhille-Campos; Kevin D. Whitley; Philipp Radler; Martin Loose; Séamus Holden; Anđela Šarić
Source: check_circle
Crossref

Complement-mediated killing of Escherichia coli by mechanical destabilization of the cell envelope

The EMBO Journal
2024-10-14 | Journal article
DOI: 10.1038/s44318-024-00266-3
Contributors: Georgina Benn; Christian Bortolini; David M Roberts; Alice L B Pyne; Séamus Holden; Bart W Hoogenboom
Source: check_circle
Crossref

Author Correction: Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis

Nature Microbiology
2024-05-15 | Journal article
DOI: 10.1038/s41564-024-01725-7
Contributors: Kevin D. Whitley; James Grimshaw; David M. Roberts; Eleni Karinou; Phillip J. Stansfeld; Séamus Holden
Source: check_circle
Crossref

Peptidoglycan synthesis drives a single population of septal cell wall synthases during division in Bacillus subtilis

Nature Microbiology
2024-03-13 | Journal article
DOI: 10.1038/s41564-024-01650-9
Contributors: Kevin D. Whitley; James Grimshaw; David M. Roberts; Eleni Karinou; Phillip J. Stansfeld; Séamus Holden
Source: check_circle
Crossref

Complement-mediated killing of bacteria by mechanical destabilization of the cell envelope

2023-12-10 | Preprint
DOI: 10.1101/2023.12.10.570986
Contributors: Georgina Benn; Christian Bortolini; David M. Roberts; Alice L. B. Pyne; Séamus Holden; Bart W. Hoogenboom
Source: check_circle
Crossref

Transertion and cell geometry organize theEscherichia colinucleoid during rapid growth

2023-10-16 | Preprint
DOI: 10.1101/2023.10.16.562172
Contributors: Christoph Spahn; Stuart Middlemiss; Estibaliz Gómez-de-Mariscal; Ricardo Henriques; Helge B. Bode; Séamus Holden; Mike Heilemann
Source: check_circle
Crossref

A one-track model for spatiotemporal coordination ofBacillus subtilisseptal cell wall synthesis

2023-06-29 | Preprint
DOI: 10.1101/2023.06.29.547024
Contributors: Kevin D. Whitley; James Grimshaw; David M. Roberts; Eleni Karinou; Phillip J. Stansfeld; Séamus Holden
Source: check_circle
Crossref

Molecular motor tug-of-war regulates elongasome cell wall synthesis dynamics inBacillus subtilis

2023-05-10 | Preprint
DOI: 10.1101/2023.05.10.540107
Contributors: Stuart Middlemiss; David M Roberts; James Grimshaw; Joshua M Edwards; Zikai Sun; Kevin D Whitley; Thierry Blu; Henrik Strahl; Séamus Holden
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Crossref

Self-organisation of mortal filaments: the role of FtsZ treadmilling in bacterial division ring formation

2023-05-09 | Preprint
DOI: 10.1101/2023.05.08.539808
Contributors: Christian Vanhille-Campos; Kevin D. Whitley; Philipp Radler; Martin Loose; Séamus Holden; Anđela Šarić
Source: check_circle
Crossref

High-resolution imaging of bacterial spatial organization with vertical cell imaging by nanostructured immobilization (VerCINI)

Nature Protocols
2022-03 | Journal article
DOI: 10.1038/s41596-021-00668-1
Contributors: Kevin D. Whitley; Stuart Middlemiss; Calum Jukes; Cees Dekker; Séamus Holden
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Crossref

Democratising deep learning for microscopy with ZeroCostDL4Mic

Nature Communications
2021 | Journal article
DOI: 10.1038/s41467-021-22518-0
EID:

2-s2.0-85104478426

Part of ISSN: 20411723
Contributors: von Chamier, L.; Laine, R.F.; Jukkala, J.; Spahn, C.; Krentzel, D.; Nehme, E.; Lerche, M.; Hernández-Pérez, S.; Mattila, P.K.; Karinou, E. et al.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

DeepBacs: Bacterial image analysis using open-source deep learning approaches

2021-11-03 | Other
DOI: 10.1101/2021.11.03.467152
Contributors: Christoph Spahn; Romain F. Laine; Pedro Matos Pereira; Estibaliz Gómez-de-Mariscal; Lucas von Chamier; Mia Conduit; Mariana Gomes de Pinho; Guillaume Jacquemet; Séamus Holden; Mike Heilemann et al.
Source: check_circle
Crossref

High-Resolution Imaging of Bacterial Spatial Organization with Vertical Cell Imaging by Nanostructured Immobilisation (VerCINI)

Preprints
2021-06-28 | Preprint
DOI: 10.20944/preprints202106.0639.v1
Source: Self-asserted source
Séamus Holden

FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in Bacillus subtilis cell division

Nature Communications
2021-04-27 | Journal article
DOI: 10.1038/s41467-021-22526-0
Contributors: Kevin D. Whitley; Calum Jukes; Nicholas Tregidgo; Eleni Karinou; Pedro Almada; Yann Cesbron; Ricardo Henriques; Cees Dekker; Séamus Holden
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Crossref
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Erratum: Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division (Science DOI: 10.1126/science.aak9973)

Science
2020 | Journal article
DOI: 10.1126/science.aba6311
EID:

2-s2.0-85089396137

Part of ISSN: 10959203 00368075
Contributors: Bisson-Filho, A.W.; Hsu, Y.-P.; Squyres, G.R.; Kuru, E.; Wu, F.; Jukes, C.; Sun, Y.; Dekker, C.; Holden, S.; VanNieuwenhze, M.S. et al.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Geometric principles underlying the proliferation of a model cell system

Nature Communications
2020 | Journal article
DOI: 10.1038/s41467-020-17988-7
EID:

2-s2.0-85089535398

Part of ISSN: 20411723
Contributors: Wu, L.J.; Lee, S.; Park, S.; Eland, L.E.; Wipat, A.; Holden, S.; Errington, J.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Virtual-SMLM, a virtual environment for real-time interactive SMLM acquisition

bioRxiv
2020-03 | Preprint
DOI: 10.1101/2020.03.05.967893
PPR: PPR116070
Source: Self-asserted source
Séamus Holden
grade
Preferred source (of 2)‎

Movement dynamics of divisome proteins and PBP2x: FtsW in cells of Streptococcus pneumoniae

Proceedings of the National Academy of Sciences of the United States of America
2019 | Journal article
DOI: 10.1073/pnas.1816018116
EID:

2-s2.0-85061872427

Part of ISSN: 10916490 00278424
Contributors: Perez, A.J.; Cesbron, Y.; Shaw, S.L.; Villicana, J.B.; Tsui, H.-C.T.; Boersma, M.J.; Ye, Z.A.; Tovpeko, Y.; Dekker, C.; Holden, S. et al.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Publisher Correction: Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software (Nature Methods, (2019), 16, 5, (387-395), 10.1038/s41592-019-0364-4)

Nature Methods
2019 | Journal article
DOI: 10.1038/s41592-019-0453-4
EID:

2-s2.0-85066019880

Part of ISSN: 15487105 15487091
Contributors: Sage, D.; Pham, T.-A.; Babcock, H.; Lukes, T.; Pengo, T.; Chao, J.; Velmurugan, R.; Herbert, A.; Agrawal, A.; Colabrese, S. et al.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

Nature Methods
2019 | Journal article
DOI: 10.1038/s41592-019-0364-4
EID:

2-s2.0-85064068699

Part of ISSN: 15487105 15487091
Contributors: Sage, D.; Pham, T.-A.; Babcock, H.; Lukes, T.; Pengo, T.; Chao, J.; Velmurugan, R.; Herbert, A.; Agrawal, A.; Colabrese, S. et al.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Constriction Rate Modulation Can Drive Cell Size Control and Homeostasis in C. crescentus

iScience
2018 | Journal article
DOI: 10.1016/j.isci.2018.05.020
EID:

2-s2.0-85064940157

Part of ISSN: 25890042
Contributors: Lambert, A.; Vanhecke, A.; Archetti, A.; Holden, S.; Schaber, F.; Pincus, Z.; Laub, M.T.; Goley, E.; Manley, S.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier
grade
Preferred source (of 3)‎

Probing the mechanistic principles of bacterial cell division with super-resolution microscopy

Current Opinion in Microbiology
2018 | Journal article
DOI: 10.1016/j.mib.2017.12.005
EID:

2-s2.0-85040331517

Part of ISSN: 18790364 13695274
Contributors: Holden, S.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Treadmilling by FtsZ filaments drives peptidoglycan synthesis and bacterial cell division

Science
2017-02-17 | Journal article
DOI: 10.1126/science.aak9973
Contributors: Alexandre W. Bisson-Filho; Yen-Pang Hsu; Georgia R. Squyres; Erkin Kuru; Fabai Wu; Calum Jukes; Yingjie Sun; Cees Dekker; Seamus Holden; Michael S. VanNieuwenhze et al.
Source: check_circle
Crossref
grade
Preferred source (of 4)‎

Functional dichotomy and distinct nanoscale assemblies of a cell cycle controlled bipolar zinc-finger regulator

eLife
2016 | Journal article
DOI: 10.7554/eLife.18647.001
DOI: 10.7554/eLife.18647
EID:

2-s2.0-85007346554

Part of ISSN: 2050084X
Contributors: Mignolet, J.; Holden, S.; Bergé, M.; Panis, G.; Eroglu, E.; Théraulaz, L.; Manley, S.; Viollier, P.H.
Source: Self-asserted source
Séamus Holden
grade
Preferred source (of 3)‎

Imaging: Super-resolution fight club

Nature Photonics
2016 | Journal article
DOI: 10.1038/nphoton.2016.22
EID:

2-s2.0-84959350715

Part of ISSN: 17494893 17494885
Contributors: Holden, S.; Sage, D.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Modularity and determinants of a (bi-) polarization control system from freeliving and obligate intracellular bacteria

eLife
2016 | Journal article
DOI: 10.7554/eLife.20640.001
EID:

2-s2.0-85007399912

Part of ISSN: 2050084X
Contributors: Bergé, M.; Campagne, S.; Mignolet, J.; Holden, S.; Théraulaz, L.; Manley, S.; Allain, F.H-T.; Viollier, P.H.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Modularity and determinants of a (bi-)polarization control system from free-living and obligate intracellular bacteria

eLife
2016-12-23 | Journal article
DOI: 10.7554/eLife.20640
Contributors: Matthieu Bergé; Sébastien Campagne; Johann Mignolet; Seamus Holden; Laurence Théraulaz; Suliana Manley; Frédéric H-T Allain; Patrick H Viollier
Source: check_circle
Crossref

Correction of a depth-dependent lateral distortion in 3D super-resolution imaging

PLoS ONE
2015 | Journal article
DOI: 10.1371/journal.pone.0142949
EID:

2-s2.0-84957947158

Part of ISSN: 19326203
Contributors: Carlini, L.; Holden, S.J.; Douglass, K.M.; Manley, S.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier
grade
Preferred source (of 2)‎

PALMsiever: A tool to turn raw data into results for single-molecule localization microscopy

Bioinformatics
2015 | Journal article
DOI: 10.1093/bioinformatics/btu720
EID:

2-s2.0-84928986930
Contributors: Pengo, T.; Holden, S.J.; Manley, S.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

3D high-density localization microscopy using hybrid astigmatic/ biplane imaging and sparse image reconstruction

Biomedical Optics Express
2014 | Journal article
DOI: 10.1364/BOE.5.003935
EID:

2-s2.0-84942368533
Contributors: Min, J.; Holden, S.J.; Carlini, L.; Unser, M.; Manley, S.; Ye, J.C.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Erratum: Defining the limits of single-molecule FRET resolution in TIRF microscopy (Biophys J. (2010) 99 (3102-3111))

Biophysical Journal
2014 | Journal article
DOI: 10.1016/j.bpj.2014.03.030
EID:

2-s2.0-84899841953
Contributors: Holden, S.J.; Uphoff, S.; Hohlbein, J.; Yadin, D.; Le Reste, L.; Britton, O.J.; Kapanidis, A.N.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

FALCON: Fast and unbiased reconstruction of high-density super-resolution microscopy data

Scientific Reports
2014 | Journal article
DOI: 10.1038/srep04577
EID:

2-s2.0-84897513305
Contributors: Min, J.; Vonesch, C.; Kirshner, H.; Carlini, L.; Olivier, N.; Holden, S.; Manley, S.; Ye, J.C.; Unser, M.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization

Proceedings of the National Academy of Sciences of the United States of America
2014 | Journal article
DOI: 10.1073/pnas.1313368111
EID:

2-s2.0-84896974722
Contributors: Holden, S.J.; Pengo, T.; Meibom, K.L.; Fernandez, C.F.; Collier, J.; Manley, S.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Photoactivated localization microscopy for cellular imaging

Neuromethods
2014 | Book
DOI: 10.1007/978-1-62703-983-3-5
EID:

2-s2.0-84896985547
Contributors: Achurra, P.; Holden, S.; Pengo, T.; Manley, S.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier
grade
Preferred source (of 3)‎

Multiscale spatial organization of RNA polymerase in escherichia coli

Biophysical Journal
2013 | Journal article
DOI: 10.1016/j.bpj.2013.05.048
EID:

2-s2.0-84879826657
Contributors: Endesfelder, U.; Finan, K.; Holden, S.J.; Cook, P.R.; Kapanidis, A.N.; Heilemann, M.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier
grade
Preferred source (of 2)‎

DAOSTORM: An algorithm for high-density super-resolution microscopy

Nature Methods
2011 | Journal article
DOI: 10.1038/nmeth0411-279
EID:

2-s2.0-79953283371
Contributors: Holden, S.J.; Uphoff, S.; Kapanidis, A.N.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Identifying molecular dynamics in single-molecule fret experiments with burst variance analysis

Biophysical Journal
2011 | Journal article
DOI: 10.1016/j.bpj.2011.01.066
EID:

2-s2.0-79953853888
Contributors: Torella, J.P.; Holden, S.J.; Santoso, Y.; Hohlbein, J.; Kapanidis, A.N.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Defining the limits of single-molecule FRET resolution in TIRF microscopy

Biophysical Journal
2010 | Journal article
DOI: 10.1016/j.bpj.2010.09.005
EID:

2-s2.0-78049317174
Contributors: Holden, S.J.; Uphoff, S.; Hohlbein, J.; Yadin, D.; Le Reste, L.; Britton, O.J.; Kapanidis, A.N.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Monitoring multiple distances within a single molecule using switchable FRET

Nature Methods
2010 | Journal article
DOI: 10.1038/nmeth.1502
EID:

2-s2.0-77957689889
Contributors: Uphoff, S.; Holden, S.J.; Le Reste, L.; Periz, J.; Van De Linde, S.; Heilemann, M.; Kapanidis, A.N.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Single-molecule DNA biosensors for protein and ligand detection

Angewandte Chemie - International Edition
2010 | Journal article
DOI: 10.1002/anie.200904597
EID:

2-s2.0-76349117468
Contributors: Lymperopoulos, K.; Crawford, R.; Torella, J.P.; Heilemann, M.; Hwang, L.C.; Holden, S.J.; Kapanidis, A.N.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Alternating-Laser Excitation and Pulsed-Interleaved Excitation of Single Molecules

Single Particle Tracking and Single Molecule Energy Transfer
2009 | Book
DOI: 10.1002/9783527628360.ch6
EID:

2-s2.0-78349262135
Contributors: Holden, S.J.; Kapanidis, A.N.
Source: Self-asserted source
Séamus Holden via Scopus - Elsevier

Peer review (9 reviews for 5 publications/grants)

Review activity for Communications biology. (2)
Review activity for Microbiology. (1)
Review activity for Nature communications (2)
Review activity for Nature microbiology. (3)
Review activity for PLoS biology. (1)
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