Erik Sedlak (0000-0003-1290-5774)

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Biography

EDUCATIONAL RECORD:
1993-1998 Ph.D. Department of Biochemistry, P.J. Šafárik University, Košice, Slovakia
Dissertation title: "Interaction of basic proteins with polyanions"
2006 Assoc. Prof. Habilitation work: “Conformational states of proteins.”
2020 DrSc. Doctoral Dissertation: “Conformational properties of cytochrome c and cytochrome c oxidase”
2023 Professor of Biochemistry at Department of Biochemistry, Faculty of Science at P.J.Šafárik University in Košice

PROFESSIONAL APPOINTMENTS:
~ 1 year Laboratorium für Biochemie, Universität Bayreuth, Germany, with Prof. Dr. Mathias Sprinzl, Dr.h.c.
~ 4.5 years Postdoctoral Research Associate in Biochemistry, The University of Texas Health Science Center, San Antonio, with Prof. Neal C. Robinson. Project: "Functional Association of Cardiolipin with Cytochrome c Oxidase and bc1."
~ 1.5 years Postdoctoral Senior Research Associate in Biochemistry, The Rice
University, Houston, USA, with Assoc. Prof. Dr. Pernilla Wittung-
Stafshede. Project: "Folding mechanisms of copper transport proteins."
~ 4 years Postdoctoral Associate in Biochemistry, The University of Zürich,
Zürich, Switzerland with Prof. Dr. Andreas Plückthun. Project:
“Conversion of integral membrane receptors into soluble forms ”
(Marie-Curie Intra-European Fellowship)
2003-2007 Head of Department of Biochemistry, P.J. Šafárik University, Košice, Slovak Republic
2013-presence Senior Research Associate at Center for Interdisciplinary Biosciences, P.J. Šafárik
University, Košice, Slovak Republic Associate Professor at Department of Biochemistry, P.J. Šafárik
2017-presence Head of Center for Interdisciplinary Biosciences, P.J. Šafárik University, Košice, Slovak
Republic (https://cib-center.org/)
2022-presence Functional position of Professor at the Department of Biochemistry PF UPJŠ

OTHER ACTIVITIES:
2017-2020 President of Slovak Biophysical Society
(http://skbs.sk/en/council-of-skbs/)
2011-2013 Editor in Chemical papers (Bratislava)
2010-2011 Associate Editor for Biochemistry in Chemical papers (Bratislava)

AWARDS:
• The best scientific work or collection of works of young scientist (under 35 years) in biophysics (granted by Slovak Biophysical Society; 2004)
• Award of Minister of Education of Slovak Republic for Science and Technique in category of Established results of young workers in science and development to 35 years; 2004.
• Marie-Curie Intra-European Fellowship (2009-2011) in laboratory of Prof. Dr. Andreas Plückthun, University of Zürich. Project: “Conversion of integral membrane receptors into soluble forms“

Grants awarded
by Slovak grant agencies:
Ministry of education of Slovak republic (VEGA) – 9 grants;
Development project in 2005 awarded by Ministry of education of Slovak republic.
Slovak Research and Development agency (APVV) – 2 grants;

by foreign agencies:
Marie-Curie Intra-European Fellowship in 2009-2011;
Deutsche Forschungsgemeinschaft in collaboration with Prof.Dr. Mathias Sprinzl, Dr.h.c., Universität Bayreuth, Germany in 2005

Activities

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Univerzita Pavla Jozefa Šafárika v Košiciach: Kosice, SK

2018-01-01 to present | Head (Center for Interdisciplinary Biosciences)

Employment

Erik Sedlak

https://orcid.org/0000-0003-1290-5774

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Biography

Activities

Employment (1)

Univerzita Pavla Jozefa Šafárika v Košiciach: Kosice, SK

Funding (14)

Study and modification of properties of spider protein overexpressed in Escherichia coli

Modification of catalytic properties of haloalkane dehalogenases by protein evolution methods

Development of efficient genetically encoded photosensitizers

eduLab - Educational Laboratory of Lab-on-a-Chip Technology for Accelerating Diagnostic Methods Innovation in Slovakia

Understanding IgG inactivation mechanism using single Hsp70 chaperone molecules and laser optical tweezers

Aggregation of immunoglobulins and prediction of their colloidal stability using advanced kinetic analysis

Functional and Structural Insights into the Phospholipid-Protein Interaction during Oxidative Stress

Conversion of integral membrane protein into water soluble form: the case of GPCR

Towards highly seletive cancer treatment: Endogenous lipoprotein-DARPin complexes as a new generation of targeted drug delivery vehicles

Conversion of integral membrane receptor into water soluble form

Functionality and Structural Integrity of Proteins in Bicelles – Implications for Mitochondrial and Amyloidogenic Proteins

Modulation of protein dynamics and catalytic properties of enzymes by Hofmeister ions

Structure-based design of Armadillo Repeat Proteins as a modular recognition system for the sequence-specific binding of peptides

Study of conformational, functional and sequential diversity and flavoproteins and selected homodimeric oxidases

Works (50 of 80)

pH modulates efficiency of singlet oxygen production by flavin cofactors

Design of <scp>AsLOV2</scp> domain as a carrier of light‐induced dissociable <scp>FMN</scp> photosensitizer

Only kosmotrope anions trigger fibrillization of the recombinant core spidroin eADF4(C16) from Araneus diadematus

Identification, characterization, and engineering of glycosylation in thrombolytics

Global analysis of kinetics reveals the role of secondary nucleation in recombinant spider silk self‐assembly

Salt-Specific Suppression of the Cold Denaturation of Thermophilic Multidomain Initiation Factor 2

Explanation of inconsistencies in the determination of human serum albumin thermal stability

Singlet oxygen quenching as a probe for cytochrome c molten globule state formation

Modulation of global stability, ligand binding and catalytic properties of trypsin by anions

Specific anion effect on properties of HRV 3C protease

Heme is responsible for enhanced singlet oxygen deactivation in cytochrome c

Purification of MBP fusion proteins using engineered DARPin affinity matrix

Early modification of cytochrome c by hydrogen peroxide triggers its fast degradation

Anion-Specific Effects on the Alkaline State of Cytochrome c

Destabilization effect of imidazolium cation-Hofmeister anion salts on cytochrome c

Photoinduced damage of AsLOV2 domain is accompanied by increased singlet oxygen production due to flavin dissociation

The Interplay among Subunit Composition, Cardiolipin Content, and Aggregation State of Bovine Heart Cytochrome c Oxidase

Conformational properties of LOV2 domain and its C450A variant within broad pH region

Ion-Specific Protein/Water Interface Determines the Hofmeister Effect on the Kinetic Stability of Glucose Oxidase

Dual effect of non-ionic detergent Triton X-100 on insulin amyloid formation

Hofmeister effect on catalytic properties of chymotrypsin is substrate-dependent

Peroxidase activity of cytochrome c in its compact state depends on dynamics of the heme region.

Modification of the kinetic stability of immunoglobulin G by solvent additives

Synergistic Effects of Copper Sites on Apparent Stability of Multicopper Oxidase, Fet3p

Role of cardiolipin in stability of integral membrane proteins

The molten-globule residual structure is critical for reflavination of glucose oxidase

Inner mechanism of protection of mitochondrial electron-transfer proteins against oxidative damage. Focus on hydrogen peroxide decomposition.

Analysis of IgG kinetic stability by differential scanning calorimetry, probe fluorescence and light scattering.

The fluorescence intensities ratio is not a reliable parameter for evaluation of protein unfolding transitions.

Correlation of lysozyme activity and stability in the presence of Hofmeister series anions.

Delipidation of cytochrome c oxidase from Rhodobacter sphaeroides destabilizes its quaternary structure

Directed evolution of G protein-coupled receptors in yeast for higher functional production in eukaryotic expression hosts.

Generation of Fluorogen-Activating Designed Ankyrin Repeat Proteins (FADAs) as Versatile Sensor Tools.

Non-two-state thermal denaturation of ferricytochrome c at neutral and slightly acidic pH values

Destabilization of the Quaternary Structure of Bovine Heart Cytochrome c Oxidase upon Removal of Tightly Bound Cardiolipin.

Lysozyme stability and amyloid fibrillization dependence on Hofmeister anions in acidic pH.

Advanced analyses of kinetic stabilities of iggs modified by mutations and glycosylation.

The kinetic stability of cytochrome C oxidase: effect of bound phospholipid and dimerization.

Rate of oxidative modification of cytochrome c by hydrogen peroxide is modulated by Hofmeister anions

Core glycan in the yeast multicopper ferroxidase, Fet3p: a case study of N-linked glycosylation, protein maturation, and stability.

Ferricytochrome c protects mitochondrial cytochrome c oxidase against hydrogen peroxide-induced oxidative damage.

Destabilization of the A1 domain in von Willebrand factor dissociates the A1A2A3 tri-domain and provokes spontaneous binding to glycoprotein Ibalpha and platelet activation under shear stress.

Polyanion hydrophobicity and protein basicity affect protein stability in protein-polyanion complexes.

Sequential dissociation of subunits from bovine heart cytochrome C oxidase by urea.

Changes in thermodynamic stability of von Willebrand factor differentially affect the force-dependent binding to platelet GPIbalpha.

Kinetics of cyanide binding as a probe of local stability/flexibility of cytochrome c.

In vitro unfolding of yeast multicopper oxidase Fet3p variants reveals unique role of each metal site.

Effect of Hofmeister ions on protein thermal stability: roles of ion hydration and peptide groups?

Stability and ATP binding of the nucleotide-binding domain of the Wilson disease protein: effect of the common H1069Q mutation.

Multidomain initiation factor 2 from Thermus thermophilus consists of the individual autonomous domains.

Peer review (5 reviews for 4 publications/grants)