Personal information

Emails & domains

Verified email domains

Keywords

Protein Engineering, Fluorescent Protein, Biosensor, Fluorescence imaging, Optogenetics

Countries

Canada

Activities

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Employment (2)

University of Alberta: Edmonton, AB, CA

2016-12-01 to present | Research Associate (Chemistry)
Employment
Source: Self-asserted source
Yi Shen

Harvard Medical School: Boston, MA, US

2015-04-01 to 2016-11-01 | Research Fellow (Infectious Disease)
Employment
Source: Self-asserted source
Yi Shen

Education and qualifications (2)

University of Alberta: Edmonton, AB, CA

2009-09-01 to 2014-12-01 | Ph.D (Chemistry)
Education
Source: Self-asserted source
Yi Shen

China Pharmaceutical University: Nanjing, Jiangsu, CN

2005-09-01 to 2009-06-30 | BSc (Biochemsitry)
Education
Source: Self-asserted source
Yi Shen

Works (31)

An automated screening platform for improving the responsiveness of genetically encoded Ca2+ biosensors in mammalian cells

Sensors & Diagnostics
2024 | Journal article
DOI: 10.1039/D4SD00138A
Contributors: Yufeng Zhao; Yi Shen; Teodor Veres; Robert E. Campbell
Source: check_circle
Crossref

A Monochromatically Excitable Green–Red Dual-Fluorophore Fusion Incorporating a New Large Stokes Shift Fluorescent Protein

Biochemistry
2024-01-02 | Journal article
DOI: 10.1021/acs.biochem.3c00451
Contributors: J. Obinna Ejike; Mayuri Sadoine; Yi Shen; Yuuma Ishikawa; Erdem Sunal; Sebastian Hänsch; Anna B. Hamacher; Wolf B. Frommer; Michael M. Wudick; Robert E. Campbell et al.
Source: check_circle
Crossref

Rational Engineering of an Improved Genetically Encoded pH Sensor Based on Superecliptic pHluorin

ACS Sensors
2023-08-25 | Journal article
DOI: 10.1021/acssensors.3c00484
Contributors: Yi Shen; Yurong Wen; Silvia Sposini; Anjali Amrapali Vishwanath; Ahmed S. Abdelfattah; Eric R. Schreiter; M. Joanne Lemieux; Jaime de Juan-Sanz; David Perrais; Robert E. Campbell
Source: check_circle
Crossref

A monochromatically excitable green-red dual-fluorophore fusion incorporating a new large Stokes shift fluorescent protein

2023-07-17 | Preprint
DOI: 10.1101/2023.07.16.549156
Contributors: J. Obinna Ejike; Mayuri Sadoine; Yi Shen; Yuuma Ishikawa; Erdem Sunal; Sebastian Hänsch; Anna B. Hamacher; Wolf B. Frommer; Michael M. Wudick; Robert E. Campbell et al.
Source: check_circle
Crossref

Author Correction: Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification

Nature Communications
2023-04-17 | Journal article
DOI: 10.1038/s41467-023-37856-4
Contributors: Franziska Bierbuesse; Anaïs C. Bourges; Vincent Gielen; Viola Mönkemöller; Wim Vandenberg; Yi Shen; Johan Hofkens; Pieter Vanden Berghe; Robert E. Campbell; Benjamien Moeyaert et al.
Source: check_circle
Crossref

Biosensor Optimization Using a Förster Resonance Energy Transfer Pair Based on mScarlet Red Fluorescent Protein and an mScarlet-Derived Green Fluorescent Protein

ACS Sensors
2023-02-24 | Journal article
DOI: 10.1021/acssensors.2c01730
Contributors: Khyati Gohil; Sheng-Yi Wu; Kei Takahashi-Yamashiro; Yi Shen; Robert E. Campbell
Source: check_circle
Crossref

A Complementary Silicon Quantum Dot-Enzyme Platform for the Selective Detection of Nitroaromatic Compounds: Explosives versus Nerve Agents

ACS Applied Nano Materials
2022-08-26 | Journal article
DOI: 10.1021/acsanm.2c03105
Contributors: Leanne A. Milburn; Christopher Jay T. Robidillo; Rochelin Dalangin; Yi Shen; Jonathan G. C. Veinot
Source: check_circle
Crossref

Biosensor optimization using a FRET pair based on mScarlet red fluorescent protein and an mScarlet-derived green fluorescent protein

2022-06-20 | Preprint
DOI: 10.1101/2022.06.20.496847
Contributors: Khyati Gohil; Sheng-Yi Wu; Kei Takahashi-Yamashiro; Yi Shen; Robert E. Campbell
Source: check_circle
Crossref

Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification

Nature Communications
2022-04-06 | Journal article
DOI: 10.1038/s41467-022-29508-w
Contributors: Franziska Bierbuesse; Anaïs C. Bourges; Vincent Gielen; Viola Mönkemöller; Wim Vandenberg; Yi Shen; Johan Hofkens; Pieter Vanden Berghe; Robert E. Campbell; Benjamien Moeyaert et al.
Source: check_circle
Crossref

Live cell tracking of macrophage efferocytosis during Drosophila embryo development in vivo

Science
2022-03-11 | Journal article
DOI: 10.1126/science.abl4430
Contributors: Michael H. Raymond; Andrew J. Davidson; Yi Shen; Daniel R. Tudor; Christopher D. Lucas; Sho Morioka; Justin S. A. Perry; Julia Krapivkina; David Perrais; Linus J. Schumacher et al.
Source: check_circle
Crossref

Photocleavable proteins that undergo fast and efficient dissociation

Chemical Science
2021 | Journal article
DOI: 10.1039/D1SC01059J
Contributors: Xiaocen Lu; Yurong Wen; Shuce Zhang; Wei Zhang; Yilun Chen; Yi Shen; M. Joanne Lemieux; Robert E. Campbell
Source: check_circle
Crossref

Design and Prototyping of Genetically Encoded Arsenic Biosensors Based on Transcriptional Regulator AfArsR

Biomolecules
2021-08-26 | Journal article
DOI: 10.3390/biom11091276
Contributors: Salma Saeed Khan; Yi Shen; Muhammad Qaiser Fatmi; Robert E. Campbell; Habib Bokhari
Source: check_circle
Crossref
grade
Preferred source (of 2)‎

Structure- and mechanism-guided design of single fluorescent protein-based biosensors

Nature Chemical Biology
2021-05 | Journal article
DOI: 10.1038/s41589-020-00718-x
Contributors: Yusuke Nasu; Yi Shen; Luke Kramer; Robert E. Campbell
Source: check_circle
Crossref

Improved Photocleavable Proteins with Faster and More Efficient Dissociation

2020-12-10 | Preprint
DOI: 10.1101/2020.12.10.419556
Contributors: Xiaocen Lu; Yurong Wen; Shuce Zhang; Wei Zhang; Yilun Chen; Yi Shen; M. Joanne Lemieux; Robert E. Campbell
Source: check_circle
Crossref

Absolute measurement of cellular activities using photochromic single-fluorophore biosensors

2020-10-29 | Preprint
DOI: 10.1101/2020.10.29.360214
Contributors: Vincent Gielen; Viola Mönkemöller; Franziska Bierbuesse; Anaïs C. Bourges; Wim Vandenberg; Yi Shen; Johan Hofkens; Pieter Vanden Berghe; Robert E. Campbell; Benjamien Moeyaert et al.
Source: check_circle
Crossref

Engineering Photosensory Modules of Non-Opsin-Based Optogenetic Actuators

International Journal of Molecular Sciences
2020-09-07 | Journal article
DOI: 10.3390/ijms21186522
Contributors: Xiaocen Lu; Yi Shen; Robert E. Campbell
Source: check_circle
Crossref
grade
Preferred source (of 2)‎

High performance intensiometric direct- and inverse-response genetically encoded biosensors for citrate

2020-04-13 | Preprint
DOI: 10.1101/2020.04.12.038547
Contributors: Yufeng Zhao; Yi Shen; Yurong Wen; Robert E. Campbell
Source: check_circle
Crossref

Genetically encoded fluorescent indicators for imaging intracellular potassium ion concentration

Communications Biology
2019-01-14 | Journal article
DOI: 10.1038/s42003-018-0269-2
Contributors: Yi Shen; Sheng-Yi Wu; Vladimir Rancic; Abhi Aggarwal; Yong Qian; Shin-Ichiro Miyashita; Klaus Ballanyi; Robert E. Campbell; Min Dong
Source: check_circle
Crossref

Structural characterisation of the catalytic domain of botulinum neurotoxin X - high activity and unique substrate specificity

Scientific Reports
2018-03-14 | Journal article
DOI: 10.1038/s41598-018-22842-4
Contributors: Geoffrey Masuyer; Sicai Zhang; Sulyman Barkho; Yi Shen; Linda Henriksson; Sara Košenina; Min Dong; Pål Stenmark
Source: check_circle
Crossref

Engineering of mCherry variants with long Stokes shift, red-shifted fluorescence, and low cytotoxicity

PloS one
2017 | Journal article
Source: Self-asserted source
Yi Shen

Identification and characterization of a novel botulinum neurotoxin

Nature Communications
2017 | Journal article
Source: Self-asserted source
Yi Shen

A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein

2017-11-02 | Other
DOI: 10.1101/213082
Contributors: Yi Shen; Hod Dana; Ahmed S. Abdelfattah; Ronak Patel; Jamien Shea; Rosana S. Molina; Bijal Rawal; Vladimir Rancic; Yu-Fen Chang; Lanshi Wu et al.
Source: check_circle
Crossref

A Tandem Green-Red Heterodimeric Fluorescent Protein with High FRET Efficiency

Chembiochem
2016 | Journal article
Source: Self-asserted source
Yi Shen

Optical Probes in Biology. Jin Zhang, Sohum Mehta, and Carsten Schultz (Eds.). CRC Press, Boca Raton, FL; 2015, 511 pages. ISBN 1466510110

Microscopy and Microanalysis
2015 | Journal article
Source: Self-asserted source
Yi Shen

Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange

Nature methods
2015 | Journal article
Source: Self-asserted source
Yi Shen

Red fluorescent proteins (RFPs) and RFP-based biosensors for neuronal imaging applications

Neurophotonics
2015 | Journal article
Source: Self-asserted source
Yi Shen

A photochromic and thermochromic fluorescent protein

RSC Advances
2014 | Journal article
Source: Self-asserted source
Yi Shen

Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications

Nature protocols
2014 | Journal article
Source: Self-asserted source
Yi Shen

pHuji, a pH-sensitive red fluorescent protein for imaging of exo- and endocytosis

J. Cell Biol.
2014 | Journal article
Source: Self-asserted source
Yi Shen

Red fluorescent genetically encoded Ca2+ indicators for use in mitochondria and endoplasmic reticulum

Biochemical Journal
2014 | Journal article
Source: Self-asserted source
Yi Shen

Portable self-contained cultures for phage and bacteria made of paper and tape

Lab on a Chip
2012 | Journal article
Source: Self-asserted source
Yi Shen

Peer review (7 reviews for 5 publications/grants)

Review activity for ACS sensors. (1)
Review activity for Analytical chemistry. (2)
Review activity for Communications biology. (1)
Review activity for Nature communications (2)
Review activity for The journal of physical chemistry. (1)
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