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Scopus Author ID: 55781805600

Biography

Ten years in the Royal Dutch Navy, 20+ years in electron microscopy.

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Employment (2)

Thermo Fisher Scientific: Eindhoven, NL

2023-01-01 to present | Senior Manager (Research & Development)
Employment
Source: Self-asserted source
Wim Hagen

EMBL Heidelberg: Heidelberg, Baden-Württemberg , DE

2012-11-04 to 2022-12-31 | Senior Technical Officer (Structural and Computational Biology)
Employment
Source: Self-asserted source
Wim Hagen

Works (50 of 52)

Items per page:
Page 1 of 2

Automated Cryo Electron Tomography Data Collection

2024 | Book chapter
DOI: 10.1007/978-3-031-51171-4_7
Contributors: Wim J. H. Hagen
Source: check_circle
Crossref

Ice thickness control and measurement in the VitroJet for time-efficient single particle structure determination

Journal of Structural Biology
2024-12 | Journal article
DOI: 10.1016/j.jsb.2024.108139
Contributors: Rene J.M. Henderikx; Maaike J.G. Schotman; Saba Shahzad; Simon A. Fromm; Daniel Mann; Julian Hennies; Thomas V. Heidler; Dariush Ashtiani; Wim J.H. Hagen; Roger J.M. Jeurissen et al.
Source: check_circle
Crossref

The architecture of Cidec-mediated interfaces between lipid droplets

Cell Reports
2023-02 | Journal article
DOI: 10.1016/j.celrep.2023.112107
Part of ISSN: 2211-1247
Contributors: Iva Ganeva; Koini Lim; Jerome Boulanger; Patrick C. Hoffmann; Olivia Muriel; Alicia C. Borgeaud; Wim J.H. Hagen; David Bousfield Savage; Wanda Kukulski
Source: Self-asserted source
Wim Hagen

Light ‘em up: efficient screening of gold grids in cryo-EM

2022-04-28 | Preprint
DOI: 10.1101/2022.04.27.489675
Contributors: Wim J.H. Hagen
Source: check_circle
Crossref

Exploring high-resolution cryo-ET and subtomogram averaging capabilities of contemporary DEDs

Journal of Structural Biology
2022-03-28 | Journal article
DOI: 10.1016/j.jsb.2022.107852
Part of ISSN: 1047-8477
Source: Self-asserted source
Wim Hagen

Artificial intelligence reveals nuclear pore complexity

2021-10-27 | Other
DOI: 10.1101/2021.10.26.465776
Contributors: Shyamal Mosalaganti; Agnieszka Obarska-Kosinska; Marc Siggel; Beata Turonova; Christian E. Zimmerli; Katarzyna Buczak; Florian H. Schmidt; Erica Margiotta; Marie-Therese Mackmull; Wim Hagen et al.
Source: check_circle
Crossref

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition.

Journal of visualized experiments : JoVE
2021-03 | Journal article
DOI: 10.3791/62383
PMID: 33818563
Contributors: Weis F; Hagen WJH; Schorb M; Mattei S
Source: Self-asserted source
Wim Hagen via Europe PubMed Central

Defocus-dependent Thon-ring fading.

Ultramicroscopy
2021-01 | Journal article
DOI: 10.1016/j.ultramic.2021.113213
PMID: 33524638
Contributors: Glaeser RM; Hagen WJH; Han BG; Henderson R; McMullan G; Russo CJ
Source: Self-asserted source
Wim Hagen via Europe PubMed Central

TEM bright field imaging of thick specimens: nodes in Thon ring patterns

Ultramicroscopy
2020 | Journal article
DOI: https://doi.org/10.1016/j.ultramic.2020.113023
Source: Self-asserted source
Wim Hagen

In-cell architecture of the nuclear pore and snapshots of its turnover

Nature
2020-10-29 | Journal article
DOI: 10.1038/s41586-020-2670-5
Contributors: Matteo Allegretti; Christian E. Zimmerli; Vasileios Rantos; Florian Wilfling; Paolo Ronchi; Herman K. H. Fung; Chia-Wei Lee; Wim Hagen; Beata Turoňová; Kai Karius et al.
Source: check_circle
Crossref

In situ structural analysis of SARS-CoV-2 spike reveals flexibility mediated by three hinges

Science
2020-10-09 | Journal article
DOI: 10.1126/science.abd5223
Contributors: Beata Turoňová; Mateusz Sikora; Christoph Schürmann; Wim J. H. Hagen; Sonja Welsch; Florian E. C. Blanc; Sören von Bülow; Michael Gecht; Katrin Bagola; Cindy Hörner et al.
Source: check_circle
Crossref

Structural impact of K63 ubiquitin on yeast translocating ribosomes under oxidative stress

Proceedings of the National Academy of Sciences
2020-09-08 | Journal article
DOI: 10.1073/pnas.2005301117
Contributors: Ye Zhou; Panagiotis L. Kastritis; Shannon E. Dougherty; Jonathan Bouvette; Allen L. Hsu; Laura Burbaum; Shyamal Mosalaganti; Stefan Pfeffer; Wim J. H. Hagen; Friedrich Förster et al.
Source: check_circle
Crossref

Combining high throughput and high quality for cryo-electron microscopy data collection.

Acta crystallographica. Section D, Structural biology
2020-07 | Journal article
DOI: 10.1107/s2059798320008347
PMID: 32744254
PMC: PMC7397495
Contributors: Weis F; Hagen WJH
Source: Self-asserted source
Wim Hagen via Europe PubMed Central

In-cell architecture of an actively transcribing-translating expressome

Science
2020-07-31 | Journal article
DOI: 10.1126/science.abb3758
Contributors: Francis J. O’Reilly; Liang Xue; Andrea Graziadei; Ludwig Sinn; Swantje Lenz; Dimitry Tegunov; Cedric Blötz; Neil Singh; Wim J. H. Hagen; Patrick Cramer et al.
Source: check_circle
Crossref

Structural basis for RNA polymerase III transcription repression by Maf1

Nature Structural & Molecular Biology
2020-03-17 | Journal article
DOI: 10.1038/s41594-020-0383-y
Contributors: Matthias K. Vorländer; Florence Baudin; Robyn D. Moir; René Wetzel; Wim J. H. Hagen; Ian M. Willis; Christoph W. Müller
Source: check_circle
Crossref

In-cell architecture of an actively transcribing-translating expressome

2020-02-28 | Preprint
DOI: 10.1101/2020.02.28.970111
Contributors: Francis J. O’Reilly; Liang Xue; Andrea Graziadei; Ludwig Sinn; Swantje Lenz; Dimitry Tegunov; Cedric Blötz; Wim J. H. Hagen; Patrick Cramer; Jörg Stülke et al.
Source: check_circle
Crossref

Benchmarking tomographic acquisition schemes for high-resolution structural biology

Nature Communications
2020-02-13 | Journal article
DOI: 10.1038/s41467-020-14535-2
Part of ISSN: 2041-1723
Source: Self-asserted source
Wim Hagen

Structural basis of p62/SQSTM1 helical filaments and their role in cellular cargo uptake

Nature Communications
2020-01-23 | Journal article
DOI: 10.1038/s41467-020-14343-8
Part of ISSN: 2041-1723
Source: Self-asserted source
Wim Hagen

Software tools for automated transmission electron microscopy

Nature Methods
2019-06-13 | Journal article
DOI: 10.1038/s41592-019-0396-9
Contributors: Martin Schorb; Isabella Haberbosch; Wim J. H. Hagen; Yannick Schwab; David N. Mastronarde
Source: check_circle
Crossref

New tools for automated high-resolution cryo-EM structure determination in RELION-3

eLife
2018-11-09 | Journal article
DOI: 10.7554/eLife.42166
Contributors: Jasenko Zivanov; Takanori Nakane; Björn O Forsberg; Dari Kimanius; Wim JH Hagen; Erik Lindahl; Sjors HW Scheres
Source: check_circle
Crossref

Molecular mechanism of promoter opening by RNA polymerase III

Nature
2018-01-17 | Journal article
DOI: 10.1038/nature25440
Source: Self-asserted source
Wim Hagen

Best practices for managing large CryoEM facilities.

Journal of structural biology
2017-09 | Journal article
DOI: 10.1016/j.jsb.2017.07.011
PMID: 28827185
PMC: PMC5605453
Source: Self-asserted source
Wim Hagen via Europe PubMed Central

Capturing protein communities by structural proteomics in a thermophilic eukaryote

Molecular Systems Biology
2017-07 | Journal article
DOI: 10.15252/msb.20167412
Contributors: Panagiotis L Kastritis; Francis J O'Reilly; Thomas Bock; Yuanyue Li; Matt Z Rogon; Katarzyna Buczak; Natalie Romanov; Matthew J Betts; Khanh Huy Bui; Wim J Hagen et al.
Source: Self-asserted source
Wim Hagen via Crossref Metadata Search

Structural insights into transcription initiation by yeast RNA polymerase I

The EMBO Journal
2017-07 | Journal article
DOI: 10.15252/embj.201796958
Contributors: Yashar Sadian; Lucas Tafur; Jan Kosinski; Arjen J Jakobi; Rene Wetzel; Katarzyna Buczak; Wim JH Hagen; Martin Beck; Carsten Sachse; Christoph W Müller
Source: Self-asserted source
Wim Hagen via Crossref Metadata Search

9Å structure of the COPI coat reveals that the Arf1 GTPase occupies two contrasting molecular environments

eLife
2017-06-16 | Journal article
DOI: 10.7554/eLife.26691
Contributors: Svetlana O Dodonova; Patrick Aderhold; Juergen Kopp; Iva Ganeva; Simone Röhling; Wim J H Hagen; Irmgard Sinning; Felix Wieland; John A G Briggs
Source: check_circle
Crossref

Structure of the hexagonal surface layer on Caulobacter crescentus cells

Nature Microbiology
2017-04-18 | Journal article
DOI: 10.1038/nmicrobiol.2017.59
Contributors: Tanmay A. M. Bharat; Danguole Kureisaite-Ciziene; Gail G. Hardy; Ellen W. Yu; Jessica M. Devant; Wim J. H. Hagen; Yves V. Brun; John A. G. Briggs; Jan Löwe
Source: check_circle
Crossref

An atomic model of HIV-1 capsid-SP1 reveals structures regulating assembly and maturation

Science
2016 | Journal article
DOI: 10.1126/science.aaf9620
EID:

2-s2.0-84978785861
Contributors: Schur, F.K.M.; Obr, M.; Hagen, W.J.H.; Wan, W.; Jakobi, A.J.; Kirkpatrick, J.M.; Sachse, C.; Kräusslich, H.-G.; Briggs, J.A.G.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

Higher-order assemblies of oligomeric cargo receptor complexes form the membrane scaffold of the Cvt vesicle

EMBO Reports
2016 | Journal article
DOI: 10.15252/embr.201541960
EID:

2-s2.0-84973300867
Contributors: Bertipaglia, C.; Schneider, S.; Jakobi, A.J.; Tarafder, A.K.; Bykov, Y.S.; Picco, A.; Kukulski, W.; Kosinski, J.; Hagen, W.J.H.; Ravichandran, A.C. et al.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

Molecular Structures of Transcribing RNA Polymerase I.

Molecular cell
2016-12 | Journal article
DOI: 10.1016/j.molcel.2016.11.013
PMID: 27867008
Source: Self-asserted source
Wim Hagen

The structure and flexibility of conical HIV-1 capsids determined within intact virions.

Science
2016-12 | Journal article
DOI: 10.1126/science.aah4972
PMID: 27980210
Source: Self-asserted source
Wim Hagen

Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging.

2016-06 | Journal article
PMID: 27313000
Contributors: Hagen WJ; Wan W; Briggs JA
Source: Self-asserted source
Wim Hagen via Europe PubMed Central

Molecular architecture of the inner ring scaffold of the human nuclear pore complex.

2016-04 | Journal article
DOI: 10.1126/science.aaf0643
PMID: 27081072
Contributors: Kosinski J; Mosalaganti S; von Appen A; Teimer R; DiGuilio AL; Wan W; Bui KH; Hagen WJ; Briggs JA; Glavy JS et al.
Source: Self-asserted source
Wim Hagen via Europe PubMed Central
grade
Preferred source (of 2)‎

A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly

Science
2015 | Journal article
DOI: 10.1126/science.aab1121
EID:

2-s2.0-84937573115
Contributors: Dodonova, S.O.; Diestelkoetter-Bachert, P.; Von Appen, A.; Hagen, W.J.H.; Beck, R.; Beck, M.; Wieland, F.; Briggs, J.A.G.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

An Organized Co-assembly of Clathrin Adaptors Is Essential for Endocytosis

Developmental Cell
2015 | Journal article
DOI: 10.1016/j.devcel.2015.02.023
EID:

2-s2.0-84928011772
Contributors: Skruzny, M.; Desfosses, A.; Prinz, S.; Dodonova, S.O.; Gieras, A.; Uetrecht, C.; Jakobi, A.J.; Abella, M.; Hagen, W.J.H.; Schulz, J. et al.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

In situ structural analysis of the human nuclear pore complex

Nature
2015 | Journal article
DOI: 10.1038/nature15381
EID:

2-s2.0-84943150449
Contributors: Von Appen, A.; Kosinski, J.; Sparks, L.; Ori, A.; DiGuilio, A.L.; Vollmer, B.; Mackmull, M.-T.; Banterle, N.; Parca, L.; Kastritis, P. et al.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

Seeing tobacco mosaic virus through direct electron detectors

Journal of Structural Biology
2015 | Journal article
DOI: 10.1016/j.jsb.2014.12.002
EID:

2-s2.0-84922595259
Contributors: Fromm, S.A.; Bharat, T.A.M.; Jakobi, A.J.; Hagen, W.J.H.; Sachse, C.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

Structure of the immature HIV-1 capsid in intact virus particles at 8.8 Å resolution

Nature
2015 | Journal article
DOI: 10.1038/nature13838
EID:

2-s2.0-84924189026
Contributors: Schur, F.K.M.; Hagen, W.J.H.; Rumlová, M.; Ruml, T.; Müller, B.; Kraüsslich, H.-G.; Briggs, J.A.G.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

The dynamic conformational landscape of γ-secretase

Journal of Cell Science
2015 | Journal article
DOI: 10.1242/jcs.164384
EID:

2-s2.0-84961331937
Contributors: Elad, N.; de Strooper, B.; Lismont, S.; Hagen, W.; Veugelen, S.; Arimon, M.; Horré, K.; Berezovska, O.; Sachse, C.; Chávez-Gutiérrez, L.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

The Selective Autophagy Receptor p62 Forms a Flexible Filamentous Helical Scaffold

Cell Reports
2015 | Journal article
DOI: 10.1016/j.celrep.2015.03.062
EID:

2-s2.0-84933679024
Contributors: Ciuffa, R.; Lamark, T.; Tarafder, A.K.; Guesdon, A.; Rybina, S.; Hagen, W.J.H.; Johansen, T.; Sachse, C.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

The structure of immature virus-like Rous sarcoma virus gag particles reveals a structural role for the p10 domain in assembly

Journal of Virology
2015 | Journal article
DOI: 10.1128/JVI.01502-15
EID:

2-s2.0-84942155961
Contributors: Schur, F.K.M.; Dick, R.A.; Hagen, W.J.H.; Vogt, V.M.; Briggs, J.A.G.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

Molecular structures of unbound and transcribing RNA polymerase III.

2015-12 | Journal article
DOI: 10.1038/nature16143
PMID: 26605533
Contributors: Hoffmann NA; Jakobi AJ; Moreno-Morcillo M; Glatt S; Kosinski J; Hagen WJ; Sachse C; Müller CW
Source: Self-asserted source
Wim Hagen via Europe PubMed Central
grade
Preferred source (of 2)‎

SNARE and regulatory proteins induce local membrane protrusions to prime docked vesicles for fast calcium-triggered fusion

EMBO Reports
2014 | Journal article
DOI: 10.1002/embr.201337807
EID:

2-s2.0-84898619506
Contributors: Bharat, T.A.M.; Malsam, J.; Hagen, W.J.H.; Scheutzow, A.; Söllner, T.H.; Briggs, J.A.G.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

Cryo-electron microscopy of tubular arrays of HIV-1 Gag resolves structures essential for immature virus assembly.

PNAS
2014-06 | Journal article
DOI: 10.1073/pnas.1401455111
PMID: 24843179
PMC: PMC4050629
Source: Self-asserted source
Wim Hagen

Determination of protein structure at 8.5Å resolution using cryo-electron tomography and sub-tomogram averaging

Journal of Structural Biology
2013 | Journal article
DOI: 10.1016/j.jsb.2013.10.015
EID:

2-s2.0-84888074636
Contributors: Schur, F.K.M.; Hagen, W.J.H.; De Marco, A.; Briggs, J.A.G.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

Integrated structural analysis of the human nuclear pore complex scaffold

Cell
2013 | Journal article
DOI: 10.1016/j.cell.2013.10.055
EID:

2-s2.0-84890101190
Contributors: Bui, K.H.; Von Appen, A.; Diguilio, A.L.; Ori, A.; Sparks, L.; Mackmull, M.-T.; Bock, T.; Hagen, W.; Andrés-Pons, A.; Glavy, J.S. et al.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

Open membranes are the precursors for assembly of large DNA viruses

Cellular Microbiology
2013 | Journal article
DOI: 10.1111/cmi.12156
EID:

2-s2.0-84885422600
Contributors: Sua;rez, C.; Welsch, S.; Chlanda, P.; Hagen, W.; Hoppe, S.; Kolovou, A.; Pagnier, I.; Raoult, D.; Krijnse Locker, J.
Source: Self-asserted source
Wim Hagen via Scopus - Elsevier

Cryo electron tomography - tilt-series of Sars-Cov-2

Dataset
EMPIAR: EMPIAR-10453
Source: check_circle
EMBL-EBI

Cryo-electron tomography and subtomogram averaging of Rous-Sarcoma-Virus deltaMBD virus-like particles

Dataset
EMPIAR: EMPIAR-10037
Source: check_circle
EMBL-EBI

Cryo-electron tomography of immature HIV-1 dMACANC VLPs

Dataset
EMPIAR: EMPIAR-10164
Source: check_circle
EMBL-EBI

Human apo-ferritin reconstructed in RELION-3.0

Dataset
EMPIAR: EMPIAR-10200
Source: check_circle
EMBL-EBI
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