Personal information

Other IDs

Scopus Author ID: 6701754402
Loop profile: 284621

Keywords

Molecular Farming, Antibodies, Biotechnology

Countries

Italy

Biography

Marcello Donini is Senior Research Scientist at Biotechnology Laboratory ENEA Casaccia Research Center ENEA, (Rome, Italy) conducting research on antibody engineering, expression of recombinant proteins in heterologous systems, Molecular Farming for the production of anticancer mAbs and veterinary vaccines.

Activities

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Employment (1)

ENEA Centro Ricerche Casaccia: Santa Maria di Galeria, IT

2004-04-01 to present (SSPT BIOAG BIOTEC)
Employment
Source: Self-asserted source
Marcello Donini

Education and qualifications (1)

ENEA Centro Ricerche Casaccia: Santa Maria di Galeria, IT

2004-04-01 to present | PhD (SSPT BIOAG BIOTEC)
Education
Source: Self-asserted source
Marcello Donini

Works (36)

The expression in plants of an engineered VP2 protein of Infectious Bursal Disease Virus induces formation of structurally heterogeneous particles that protect from a very virulent viral strain

PLOS ONE
2021-02-16 | Journal article
DOI: 10.1371/journal.pone.0247134
Part of ISSN: 1932-6203
Source: Self-asserted source
Marcello Donini

Current state-of-the-art in the use of plants for the production of recombinant vaccines against infectious bursal disease virus

Applied Microbiology and Biotechnology
2020 | Journal article
DOI: 10.1007/s00253-020-10397-2
EID:

2-s2.0-85078322489

Part of ISBN:

14320614 01757598
Contributors: Rage, E.; Marusic, C.; Lico, C.; Baschieri, S.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Establishment of Hairy Root Cultures for the Production of Biopharmaceuticals and Optimization of Methods for Recombinant Protein Secretion in the Culture Medium

Hairy Root Cultures Based Applications
2020 | Book chapter
DOI: 10.1007/978-981-15-4055-4_11
Part of ISBN: 9789811540547
Part of ISBN: 9789811540554
Part of ISSN: 2523-8442
Part of ISSN: 2523-8450
Source: Self-asserted source
Marcello Donini

Optimisation of PD-FcY veterinary antigen secretion from Nicotiana benthamiana hairy roots and purification from the culture medium

Plant Cell, Tissue and Organ Culture
2020 | Journal article
DOI: 10.1007/s11240-020-01826-1
EID:

2-s2.0-85083792538

Part of ISBN:

15735044 01676857
Contributors: Rage, E.; Marusic, C.; Lico, C.; Salzano, A.M.; Scaloni, A.; Baschieri, S.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Functional characterization of a plant-produced infectious bursal disease virus antigen fused to the constant region of avian IgY immunoglobulins

Applied Microbiology and Biotechnology
2019 | Journal article
DOI: 10.1007/s00253-019-09992-9
EID:

2-s2.0-85069456083

Part of ISBN:

14320614 01757598
Contributors: Rage, E.; Drissi Touzani, C.; Marusic, C.; Lico, C.; Göbel, T.; Bortolami, A.; Bonfante, F.; Salzano, A.M.; Scaloni, A.; Fellahi, S. et al.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Current state-of-the-art in plant-based antibody production systems

2019-03 | Journal article
DOI: 10.1007/s10529-019-02651-z
Part of ISSN: 0141-5492
Part of ISSN: 1573-6776
Source: Self-asserted source
Marcello Donini
grade
Preferred source (of 2)‎

Enhancing the Secretion of a Glyco-Engineered Anti-CD20 scFv-Fc Antibody in Hairy Root Cultures

2019-03 | Journal article
DOI: 10.1002/biot.201800081
Part of ISSN: 1860-6768
Source: Self-asserted source
Marcello Donini
grade
Preferred source (of 2)‎

Hairy roots as bioreactors for the production of biopharmaceuticals

Hairy Roots: An Effective Tool of Plant Biotechnology
2018 | Book
Part of DOI: 10.1007/978-981-13-2562-5_9
EID:

2-s2.0-85082689063
Contributors: Donini, M.; Marusic, C.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

N-glycan engineering of a plant-produced anti-CD20-hIL-2 immunocytokine significantly enhances its effector functions

Biotechnology and Bioengineering
2018 | Journal article
DOI: 10.1002/bit.26503
EID:

2-s2.0-85041057399
Contributors: Marusic, C.; Pioli, C.; Stelter, S.; Novelli, F.; Lonoce, C.; Morrocchi, E.; Benvenuto, E.; Salzano, A.M.; Scaloni, A.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Omics approaches on fresh-cut lettuce reveal global molecular responses to sodium hypochlorite and peracetic acid treatment

Journal of the Science of Food and Agriculture
2018 | Journal article
DOI: 10.1002/jsfa.8521
EID:

2-s2.0-85037615638
Contributors: Daddiego, L.; Bianco, L.; Capodicasa, C.; Carbone, F.; Dalmastri, C.; Daroda, L.; Del Fiore, A.; De Rossi, P.; Di Carli, M.; Donini, M. et al.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

An accessory protease inhibitor to increase the yield and quality of a tumour-targeting mAb in nicotiana benthamiana leaves

PLoS ONE
2016 | Journal article
DOI: 10.1371/journal.pone.0167086
EID:

2-s2.0-84997525183
Contributors: Jutras, P.V.; Marusic, C.; Lonoce, C.; Deflers, C.; Goulet, M.-C.; Benvenuto, E.; Michaud, D.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Production of a tumour-targeting antibody with a human-compatible glycosylation profile in N. benthamiana hairy root cultures

Biotechnology Journal
2016 | Journal article
DOI: 10.1002/biot.201500628
EID:

2-s2.0-84985914249
Contributors: Lonoce, C.; Salem, R.; Marusic, C.; Jutras, P.V.; Scaloni, A.; Salzano, A.M.; Lucretti, S.; Steinkellner, H.; Benvenuto, E.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Production of an active anti-CD20-hIL-2 immunocytokine in Nicotiana benthamiana

Plant Biotechnology Journal
2016 | Journal article
DOI: 10.1111/pbi.12378
EID:

2-s2.0-84955191466
Contributors: Marusic, C.; Novelli, F.; Salzano, A.M.; Scaloni, A.; Benvenuto, E.; Pioli, C.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Antibody proteolysis: a common picture emerging from plants

Bioengineered
2015 | Journal article
DOI: 10.1080/21655979.2015.1067740
EID:

2-s2.0-85018224503
Contributors: Donini, M.; Lombardi, R.; Lonoce, C.; Di Carli, M.; Marusic, C.; Morea, V.; Di Micco, P.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Bacterial community and proteome analysis of fresh-cut lettuce as affected by packaging

FEMS Microbiology Letters
2015 | Journal article
DOI: 10.1093/femsle/fnv209
EID:

2-s2.0-84960083476
Contributors: Di Carli, M.; De Rossi, P.; Paganin, P.; Del Fiore, A.; Lecce, F.; Capodicasa, C.; Bianco, L.; Perrotta, G.; Mengoni, A.; Bacci, G. et al.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Site-specific proteolytic degradation of IgG monoclonal antibodies expressed in tobacco plants

Plant Biotechnology Journal
2015 | Journal article
DOI: 10.1111/pbi.12266
EID:

2-s2.0-84922753792
Contributors: Hehle, V.K.; Lombardi, R.; van Dolleweerd, C.J.; Paul, M.J.; Di Micco, P.; Morea, V.; Benvenuto, E.; Donini, M.; Ma, J.K.-C.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Case study 2: Plant-made HIV vaccines and neutralizing antibodies

Plant-derived Pharmaceuticals: Principles and Applications for Developing Countries
2014 | Book
EID:

2-s2.0-84927970917
Contributors: Marusic, C.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Proteome changes induced by c-myb silencing in human chronic myeloid leukemia cells suggest molecular mechanisms and putative biomarkers of hematopoietic malignancies

Journal of Proteomics
2014 | Journal article
DOI: 10.1016/j.jprot.2013.10.040
EID:

2-s2.0-84889671427
Contributors: Di Carli, M.; Tanno, B.; Capodicasa, C.; Villani, M.E.; Salzano, A.M.; Scaloni, A.; Raschellà, G.; Benvenuto, E.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Structure-based design and experimental engineering of a plant virus nanoparticle for the presentation of immunogenic epitopes and as a drug carrier

Journal of Biomolecular Structure and Dynamics
2014 | Journal article
DOI: 10.1080/07391102.2013.785920
EID:

2-s2.0-84892992911
Contributors: Arcangeli, C.; Circelli, P.; Donini, M.; Aljabali, A.A.A.; Benvenuto, E.; Lomonossoff, G.P.; Marusic, C.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Production of different glycosylation variants of the tumour-targeting mAb H10 in Nicotiana benthamiana: Influence on expression yield and antibody degradation

Transgenic Research
2012 | Journal article
DOI: 10.1007/s11248-012-9587-1
EID:

2-s2.0-84865978982
Contributors: Lombardi, R.; Donini, M.; Villani, M.E.; Brunetti, P.; Fujiyama, K.; Kajiura, H.; Paul, M.; Ma, J.K.-C.; Benvenuto, E.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Recent insights into plant-virus interactions through proteomic analysis

Journal of Proteome Research
2012 | Journal article
DOI: 10.1021/pr300494e
EID:

2-s2.0-84867466100
Contributors: Di Carli, M.; Benvenuto, E.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Rapid and high-yield production of immunotherapeutic plant-made antibodies

Plant-derived Vaccines: Technologies and Applications
2011 | Book
DOI: 10.2217/EBO.11.76
EID:

2-s2.0-84957021031
Contributors: Capodicasa, C.; Donini, M.; Villani, M.E.; Benvenuto, E.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Efficient Agrobacterium-based transient expression system for the production of biopharmaceuticals in plants

Bioengineered Bugs
2010 | Journal article
DOI: 10.4161/bbug.1.3.11722
EID:

2-s2.0-79851482941
Contributors: Circelli, P.; Donini, M.; Villani, M.E.; Benvenuto, E.; Marusic, C.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Optimisation of the purification process of a tumour-targeting antibody produced in N. benthamiana using vacuum-agroinfiltration

Transgenic Research
2010 | Journal article
DOI: 10.1007/s11248-010-9382-9
EID:

2-s2.0-78149470963
Contributors: Lombardi, R.; Villani, M.E.; Di Carli, M.; Brunetti, P.; Benvenuto, E.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Proteomic analysis of the plant-virus interaction in cucumber mosaic virus (CMV) resistant transgenic tomato

Journal of Proteome Research
2010 | Journal article
DOI: 10.1021/pr100487x
EID:

2-s2.0-78149377372
Contributors: Di Carli, M.; Villani, M.E.; Bianco, L.; Lombardi, R.; Perrotta, G.; Benvenuto, E.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Transient expression systems for plant-derived biopharmaceuticals

Expert Review of Vaccines
2010 | Journal article
DOI: 10.1586/erv.10.85
EID:

2-s2.0-77955260676
Contributors: Komarova, T.V.; Baschieri, S.; Donini, M.; Marusic, C.; Benvenuto, E.; Dorokhov, Y.L.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

High-level HIV-1 Nef transient expression in Nicotiana benthamiana using the P19 gene silencing suppressor protein of Artichoke Mottled Crinckle Virus

BMC Biotechnology
2009 | Journal article
DOI: 10.1186/1472-6750-9-96
EID:

2-s2.0-71549120129
Contributors: Lombardi, R.; Circelli, P.; Villani, M.E.; Buriani, G.; Nardi, L.; Coppola, V.; Bianco, L.; Benvenuto, E.; Donini, M.; Marusic, C.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Plant pharming of a full-sized, tumour-targeting antibody using different expression strategies

Plant Biotechnology Journal
2009 | Journal article
DOI: 10.1111/j.1467-7652.2008.00371.x
EID:

2-s2.0-57649121662
Contributors: Villani, M.E.; Morgun, B.; Brunetti, P.; Marusic, C.; Lombardi, R.; Pisoni, I.; Bacci, C.; Desiderio, A.; Benvenuto, E.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Plant-based strategies aimed at expressing HIV antigens and neutralizing antibodies at high levels. Nef as a case study

Transgenic Research
2009 | Journal article
DOI: 10.1007/s11248-009-9244-5
EID:

2-s2.0-69449107844
Contributors: Marusic, C.; Vitale, A.; Pedrazzini, E.; Donini, M.; Frigerio, L.; Bock, R.; Dix, P.J.; McCabe, M.S.; Bellucci, M.; Benvenuto, E.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Humanization of a highly stable single-chain antibody by structure-based antigen-binding site grafting

Molecular Immunology
2008 | Journal article
DOI: 10.1016/j.molimm.2008.01.016
EID:

2-s2.0-40849104794
Contributors: Villani, M.E.; Morea, V.; Consalvi, V.; Chiaraluce, R.; Desiderio, A.; Benvenuto, E.; Donini, M.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Validation of a stable recombinant antibodies repertoire for the direct selection of functional intracellular reagents

Journal of Immunological Methods
2008 | Journal article
DOI: 10.1016/j.jim.2007.09.003
EID:

2-s2.0-36849005918
Contributors: Villani, M.E.; Carli, M.D.; Donini, M.; Traversini, G.; Lico, C.; Franconi, R.; Benvenuto, E.; Desiderio, A.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Peptide display on Potato virus X: Molecular features of the coat protein-fused peptide affecting cell-to-cell and phloem movement of chimeric virus particles

Journal of General Virology
2006 | Journal article
DOI: 10.1099/vir.0.82097-0
EID:

2-s2.0-33749065356
Contributors: Lico, C.; Capuano, F.; Renzone, G.; Donini, M.; Marusic, C.; Scaloni, A.; Benvenuto, E.; Baschieri, S.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Production of an engineered killer peptide in Nicotiana benthamiana by using a Potato virus X expression system

Applied and Environmental Microbiology
2005 | Journal article
DOI: 10.1128/AEM.71.10.6360-6367.2005
EID:

2-s2.0-26844580747
Contributors: Donini, M.; Lico, C.; Baschieri, S.; Conti, S.; Magliani, W.; Polonelli, L.; Benvenuto, E.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Engineering stable cytoplasmic intrabodies with designed specificity

Journal of Molecular Biology
2003 | Journal article
DOI: 10.1016/S0022-2836(03)00530-8
EID:

2-s2.0-0037785117
Contributors: Donini, M.; Morea, V.; Desiderio, A.; Pashkoulov, D.; Villani, M.E.; Tramontano, A.; Benvenuto, E.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

A single-chain antibody fragment is functionally expressed in the cytoplasm of both Escherichia coli and transgenic plants

European Journal of Biochemistry
1999 | Journal article
DOI: 10.1046/j.1432-1327.1999.00443.x
EID:

2-s2.0-0033152614
Contributors: Tavladoraki, P.; Girotti, A.; Donini, M.; Arias, F.J.; Mancini, C.; Morea, V.; Chiaraluce, R.; Consalvi, V.; Benvenuto, E.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Erratum: A single-chain antibody fragment is functionally expressed in the cytoplasm of both Escherichia coli and transgenic plants (European Journal of Biochemistry (1999) 262 (617-624))

European Journal of Biochemistry
1999 | Journal article
DOI: 10.1046/j.1432-1327.1999.00662.x
EID:

2-s2.0-0033565741
Contributors: Tavlardoraki, P.; Girotti, A.; Donini, M.; Arias, F.J.; Mancini, C.; Morea, V.; Chiaraluce, R.; Consalvi, V.; Benvenuto, E.
Source: Self-asserted source
Marcello Donini via Scopus - Elsevier

Peer review (4 reviews for 3 publications/grants)

Review activity for Molecular biology reports. (1)
Review activity for Plant cell reports. (1)
Review activity for Revista argentina de microbiología. (2)
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