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Viral Immunology, Innate Immuniy , NK cells , Cytokine responses, Cytokine signaling , Inflammatory responses , Acute phase response
United States, Israel

Biography

I have been part of the field of immunology for the past fifteen years. Previously, my studies were focused on researching the anti-inflammatory properties of the acute phase protein α-1-antitrypsin (AAT) in the context of acute inflammatory responses post transplantation mediated by damage associated molecular patterns (DAMPs). These inflammatory responses can also be associated with autoinflammatory and autoimmune conditions. Specifically, I investigated the cellular events which lead to the extracellular presence of DAMP, in addition to examining the proinflammatory effect of three specific DAMP molecules: 1. Heat shock proteins (HSPs), gp96 and HSP70 and 2. the histone like protein high mobility group box 1 (HMGB1). For example, in a previous report we demonstrated that human AAT (hAAT) inhibits extracellular gp96 inflammatory mediated responses in vivo and in vitro. Furthermore, we showed that hAAT regulated the immune response during a bacterial infection (Kaner and Ochayon et al. J. Infec Dis,2015). In addition, I was involved in several studies demonstrating hAAT immune-regulatory behavior. I have investigated the role of the inflammatory response in animal models associated with allogeneic and syngeneic transplantation, vaccination, and bacterial infection. My training continued as post graduate fellow, I have expanded my experimental horizons and examined the inflammatory milieu associated with vaccine response (under the guidance of Dr. Waggoner). I questioned the role of mixed inflammatory environment which characterizes respiratory conditions such as severe asthma and COPD on NK cells functionality. Specifically, we investigated how a combination of type 1 (IL-12) and type 2 (IL-33) cytokines contribute to an enhanced cytokine production by human NK cells, we also showed that molecular mechanism which enabled this responses was via p38 MAPK pathway as well as ATF2 (Ochayon et al. JLB, 2020). Subsequently, my training continues in the Asthma division at CCHMC under the guidance of Dr. Hershey. Since early life occurrence of atopic dermatitis (AD) is strongly associated with the prevalence of childhood asthma. As part of the MPAACH cohort we are currently examining the phenotype of NK and T cells in addition to participants clinical data. Recently, we reported that NK cells derived from AD patients with allergen sensitized compared to non-allergen sensitized showed reduced expression of NKG2D at subsequent clinical visits. In addition, NK cells derived from allergen sensitized children showed reduced cytotoxicity and hyper-inflammatory behavior characterized by increased expression of TNF-alpha. There are additional ongoing studies associated to the role of NK cells in childhood AD and asthma, in addition to the effect of non-canonical cytokine combinations on NK cells functionality.

Activities

Employment (2)

Cincinnati Children's Hospital Medical Center: Cincinnati, Ohio, US

2021-03-14 to present | Research Associate (Asthma research )
Employment
Source: Self-asserted source
David Ohayon

Cincinnati Children's Hospital Medical Center: Cincinnati, OH, US

2015-10-12 to 2021-03-12 | Research Fellow (Center for Autoimmune Genomics and Etiology)
Employment
Source: Self-asserted source
David Ohayon

Education and qualifications (3)

Ben-Gurion University of the Negev Faculty of Health Sciences: Beer Sheva, Southern, IL

2010-10-02 to 2015-07-01 | PhD (Clinical Pharmacologu and Biochemistry )
Education
Source: Self-asserted source
David Ohayon

Ben-Gurion University of the Negev Faculty of Health Sciences: Beer Sheva, Southern, IL

2008-10-01 to 2010-10-01 | MSc (Clinical Pharmacologu and Biochemistry)
Education
Source: Self-asserted source
David Ohayon

Tel Hai Academic College: Upper Galilee, Northen , IL

2005-10-01 to 2008-09-15 | Bsc (Biotechnology)
Education
Source: Self-asserted source
David Ohayon

Professional activities (2)

American Heart Association: TX, TX, US

2017-01-01 to 2020-12-31
Membership
Source: Self-asserted source
David Ohayon

American Association of Immunologists: Md., Md., US

2017-01-01 to 2020-12-31
Membership
Source: Self-asserted source
David Ohayon

Funding (1)

Synergistic induction of NK cell IFN-y and TNF-a in COPD

2017-07 to 2018-06 | Award
Cincinnati Children’s Research Foundation (OH, OH, US)
Source: Self-asserted source
David Ohayon

Works (18)

Progressive accumulation of hyperinflammatory NKG2D low NK cells in early childhood severe atopic dermatitis

Science Immunology
2024-09-02 | Journal article
Contributors: David E. Ochayon; Stanley B. DeVore; Wan-Chi Chang; Durga Krishnamurthy; Harsha Seelamneni; Brittany Grashel; Daniel Spagna; Sandra Andorf; Lisa J. Martin; Jocelyn M. Biagini et al.
Source: check_circle
Crossref

Progressive accumulation of hyperinflammatory NKG2DlowNK cells in early childhood severe atopic dermatitis

2023-06-05 | Preprint
Contributors: David E. Ochayon; Stanley B. DeVore; Wan-Chi Chang; Durga Krishnamurthy; Harsha Seelamneni; Brittany Grashel; Daniel Spagna; Sandra Andorf; Lisa J. Martin; Jocelyn M. Biagini et al.
Source: check_circle
Crossref

High-resolution promoter interaction analysis in Type 3 Innate Lymphoid Cells implicates Batten Disease geneCLN3in Crohn’s Disease aetiology

2022-10-21 | Preprint
Contributors: Valeriya Malysheva; Helen Ray-Jones; Tareian A. Cazares; Owen Clay; David Ohayon; Pavel Artemov; Joseph A. Wayman; Monica Della Rosa; Carmen Petitjean; Clarissa Booth et al.
Source: check_circle
Crossref

The Promise and Peril of Natural Killer Cell Therapies in Pulmonary Infection.

Immunity
2020-05-13 | Journal article
Source: Self-asserted source
David Ohayon

IL-33 promotes type 1 cytokine expression via p38 MAPK in human NK cells

Journal of Leukocyte Biology
2020-04-01 | Journal article
Contributors: David E. Ochayon; Ayad Ali; Pablo C. Alarcon; Durga Krishnamurthy; Leah C. Kottyan; Michael T. Borchers; Stephen N. Waggoner
Source: check_circle
Crossref

Dynamic Changes in Natural Killer Cell Subset Frequencies in the Absence of Cytomegalovirus Infection.

Frontiers in immunology
2019-11-22 | Journal article
Source: Self-asserted source
David Ohayon

Characterization of Transcriptional Regulatory Networks that Promote and Restrict Identities and Functions of Intestinal Innate Lymphoid Cells.

Immunity
2019-07-02 | Journal article
Source: Self-asserted source
David Ohayon

Point Mutation of a Non-Elastase-Binding Site in Human α1-Antitrypsin Alters Its Anti-Inflammatory Properties.

Frontiers in immunology
2018-05-01 | Journal article
Source: Self-asserted source
David Ohayon

Exploration of α1-antitrypsin treatment protocol for islet transplantation: dosing plan and route of administration.

The Journal of pharmacology and experimental therapeutics
2016-11-07 | Journal article
Source: Self-asserted source
David Ohayon

Correspondence of Neutralizing Humoral Immunity and CD4 T Cell Responses in Long Recovered Sudan Virus Survivors.

Viruses
2016-05-11 | Journal article
Source: Self-asserted source
David Ohayon

Experimental Support for the Ecoimmunity Theory: Distinct Phenotypes of Nonlymphocytic Cells in SCID and Wild-Type Mice.

Cell transplantation
2016-02-02 | Journal article
Source: Self-asserted source
David Ohayon

Acute-phase protein α1-anti-trypsin: diverting injurious innate and adaptive immune responses from non-authentic threats.

Clinical and experimental immunology
2015-02-01 | Journal article
Source: Self-asserted source
David Ohayon

Immune memory to Sudan virus: comparison between two separate disease outbreaks.

Viruses
2015-01-06 | Journal article
Source: Self-asserted source
David Ohayon

Acute phase protein α1-antitrypsin reduces the bacterial burden in mice by selective modulation of innate cell responses.

The Journal of infectious diseases
2014-11-10 | Journal article
Source: Self-asserted source
David Ohayon

α1-antitrypsin increases interleukin-1 receptor antagonist production during pancreatic islet graft transplantation.

Cellular & molecular immunology
2014-04-07 | Journal article
Source: Self-asserted source
David Ohayon

Human α1-antitrypsin modifies B-lymphocyte responses during allograft transplantation.

Immunology
2013-11-01 | Journal article
Source: Self-asserted source
David Ohayon

Human α1-Antitrypsin Binds to Heat-Shock Protein gp96 and Protects from Endogenous gp96-Mediated Injury In vivo.

Frontiers in immunology
2013-10-28 | Journal article
Source: Self-asserted source
David Ohayon

Persistent immune responses after Ebola virus infection.

The New England journal of medicine
2013-08-01 | Journal article
Source: Self-asserted source
David Ohayon