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Ai Lab at University of Virginia

Other IDs

ResearcherID: A-7790-2016
Loop profile: 115760

Keywords

fluorescent biosensors, protein engineering, optical bioimaging

Countries

United States

Activities

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Employment (4)

University of Virginia: Charlottesville, VA, US

Employment
Source: check_circle
University of Virginia

University of Virginia: Charlottesville, VA, US

2017-08-01 to present | Associate Professor of Molecular Physiology & Biophysics, Chemistry (Center for Membrane and Cell Physiology)
Employment
Source: Self-asserted source
Huiwang Ai

University of California Riverside: Riverside, CA, US

2011-07-01 to 2017-07-31 | Assistant Professor (Chemistry)
Employment
Source: Self-asserted source
Huiwang Ai

Scripps Research Institute: La Jolla, CA, US

2008-06-28 to 2011-06-22 | Research Associate (Chemistry)
Employment
Source: Self-asserted source
Huiwang Ai

Education and qualifications (2)

University of Alberta: Edmonton, AB, CA

2003-09-01 to 2008-05-02 | Ph.D. (Chemistry)
Education
Source: Self-asserted source
Huiwang Ai

Tsinghua University: Beijing, Beijing, CN

1999-09-01 to 2003-07-14 | B.S. (Chemistry)
Education
Source: Self-asserted source
Huiwang Ai

Works (50 of 62)

Items per page:
Page 1 of 2

Engineering and Characterization of 3-Aminotyrosine-Derived Red Fluorescent Variants of Circularly Permutated Green Fluorescent Protein

Biosensors
2024-01 | Journal article | Author
DOI: 10.3390/bios14010054
Contributors: Hao Zhang; Xiaodong Tian; Jing Zhang; Huiwang Ai
Source: check_circle
Multidisciplinary Digital Publishing Institute

Circularly Permuted Far-Red Fluorescent Proteins

Biosensors
2021-11 | Journal article | Author
DOI: 10.3390/bios11110438
Contributors: Tianchen Wu; Yu Pang; Huiwang Ai
Source: check_circle
Multidisciplinary Digital Publishing Institute

Genetically Encoded, Photostable Indicators to Image Dynamic Zn2+ Secretion of Pancreatic Islets.

Analytical chemistry
2019-09-10 | Journal article
DOI: 10.1021/acs.analchem.9b01802
PMID: 31475537
Source: Self-asserted source
Huiwang Ai

Enabling technologies in super-resolution fluorescence microscopy: reporters, labeling, and methods of measurement.

Current opinion in structural biology
2019-06-04 | Journal article
DOI: 10.1016/j.sbi.2019.05.001
PMID: 31175034
Source: Self-asserted source
Huiwang Ai

A Genetically Encoded, Ratiometric Fluorescent Biosensor for Hydrogen Sulfide.

ACS sensors
2019-05-23 | Journal article
DOI: 10.1021/acssensors.9b00400
PMID: 31083907
PMC: PMC6599572
Source: Self-asserted source
Huiwang Ai

Molecular Tools to Generate Reactive Oxygen Species in Biological Systems.

Bioconjugate chemistry
2019-04-22 | Journal article
DOI: 10.1021/acs.bioconjchem.9b00191
PMID: 30986044
PMC: PMC6528174
Source: Self-asserted source
Huiwang Ai

ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging.

ACS chemical biology
2019-04-17 | Journal article
DOI: 10.1021/acschembio.9b00150
PMID: 30969754
PMC: PMC6528180
Source: Self-asserted source
Huiwang Ai

Identification of Factors Complicating Bioluminescence Imaging.

Biochemistry
2019-03-11 | Journal article
DOI: 10.1021/acs.biochem.8b01303
PMID: 30810040
PMC: PMC6435419
Source: Self-asserted source
Huiwang Ai

Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.

Annual review of analytical chemistry (Palo Alto, Calif.)
2019-02-20 | Journal article
DOI: 10.1146/annurev-anchem-061318-115027
PMID: 30786216
PMC: PMC6565457
Source: Self-asserted source
Huiwang Ai

A Sensitive Near-Infrared Fluorescent Sensor for Mitochondrial Hydrogen Sulfide.

ACS sensors
2018-05-03 | Journal article
DOI: 10.1021/acssensors.8b00142
PMID: 29701949
PMC: PMC5970079
Source: Self-asserted source
Huiwang Ai

An Intramolecular Interaction of UHRF1 Reveals Dual Control for Its Histone Association.

Structure (London, England : 1993)
2018-01-25 | Journal article
DOI: 10.1016/j.str.2017.12.016
PMID: 29395786
PMC: PMC5803408
Source: Self-asserted source
Huiwang Ai

Photocontrol of the Src Kinase in Mammalian Cells with a Photocaged Intein

Split Inteins: Methods and Protocols
2017 | Journal article
Source: Self-asserted source
Huiwang Ai

Illuminating Brain Activities with Fluorescent Protein-Based Biosensors.

Chemosensors (Basel, Switzerland)
2017-11-28 | Journal article
DOI: 10.3390/chemosensors5040032
PMID: 29250483
PMC: PMC5729940
Source: Self-asserted source
Huiwang Ai

Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging.

Nature methods
2017-09-04 | Journal article
DOI: 10.1038/nmeth.4400
PMID: 28869756
PMC: PMC5678970
Source: Self-asserted source
Huiwang Ai

Monitoring thioredoxin redox with a genetically encoded red fluorescent biosensor.

Nature chemical biology
2017-06-26 | Journal article
DOI: 10.1038/nchembio.2417
PMID: 28671680
PMC: PMC5605834
Source: Self-asserted source
Huiwang Ai

A Genetically Encoded FRET Sensor for Hypoxia and Prolyl Hydroxylases

ACS Chemical Biology
2016 | Journal article
Source: Self-asserted source
Huiwang Ai

A membrane-activatable near-infrared fluorescent probe with ultra-photostability for mitochondrial membrane potentials

Analyst
2016 | Journal article
Source: Self-asserted source
Huiwang Ai

A Novel Optical Crosslinking Probe with a

Acta Physico-Chimica Sinica
2016 | Journal article
Source: Self-asserted source
Huiwang Ai

Development of redox-sensitive red fluorescent proteins for imaging redox dynamics in cellular compartments

Analytical and bioanalytical chemistry
2016 | Journal article
Source: Self-asserted source
Huiwang Ai

Erratum to: Development of redox-sensitive red fluorescent proteins for imaging redox dynamics in cellular compartments

Analytical and bioanalytical chemistry
2016 | Journal article
Source: Self-asserted source
Huiwang Ai

Single Fluorescent Protein-Based Indicators for Zinc Ion (Zn2+)

Analytical Chemistry
2016 | Journal article
Source: Self-asserted source
Huiwang Ai

The N--B Interaction through a Water Bridge: Understanding the Chemoselectivity of a Fluorescent Protein Based Probe for Peroxynitrite

Journal of the American Chemical Society
2016 | Journal article
Source: Self-asserted source
Huiwang Ai

Development of Fluorescent Probes for the Detection of Peroxynitrite

Peroxynitrite Detection in Biological Media
2015 | Book chapter
Source: Self-asserted source
Huiwang Ai

Expanding the Genetic Code for a Dinitrophenyl Hapten

ChemBioChem
2015 | Journal article
Source: Self-asserted source
Huiwang Ai

Fluorescent-protein-based probes: general principles and practices

Analytical and bioanalytical chemistry
2015 | Journal article
Source: Self-asserted source
Huiwang Ai

Light activation of protein splicing with a photocaged fast intein

Journal of the American Chemical Society
2015 | Journal article
Source: Self-asserted source
Huiwang Ai

Monitoring redox dynamics in living cells with a redox-sensitive red fluorescent protein

Analytical chemistry
2015 | Journal article
Source: Self-asserted source
Huiwang Ai

Open Access Editorial Acknowledgement to Reviewers of Sensors in 2014

Sensors
2015 | Journal article
Source: Self-asserted source
Huiwang Ai

Study of the binding energies between unnatural amino acids and engineered orthogonal tyrosyl-trna synthetases

Scientific reports
2015 | Journal article
Source: Self-asserted source
Huiwang Ai

A highly efficient oxidative condensation reaction for selective protein conjugation

Chemical Communications
2014 | Journal article
Source: Self-asserted source
Huiwang Ai

A highly responsive and selective fluorescent probe for imaging physiological hydrogen sulfide

Biochemistry
2014 | Journal article
Source: Self-asserted source
Huiwang Ai

Engineering and characterizing monomeric fluorescent proteins for live-cell imaging applications

Nature protocols
2014 | Journal article
Source: Self-asserted source
Huiwang Ai

Evolution of Iron (II)-Finger Peptides by Using a Bipyridyl Amino Acid

ChemBioChem
2014 | Journal article
Source: Self-asserted source
Huiwang Ai

Fluorescent protein based probes for redox signaling molecules and metal ions

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
2014 | Conference paper
Source: Self-asserted source
Huiwang Ai

Fluorescent Sensors for Biological Applications

2014 | Other
Source: Self-asserted source
Huiwang Ai

Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells

Proceedings of the National Academy of Sciences
2013 | Journal article
Source: Self-asserted source
Huiwang Ai

Genetically encoded fluorescent probe for the selective detection of peroxynitrite

Journal of the American Chemical Society
2013 | Journal article
Source: Self-asserted source
Huiwang Ai

Genetically encoded fluorescent redox probes

Sensors
2013 | Journal article
Source: Self-asserted source
Huiwang Ai

Biochemical analysis with the expanded genetic lexicon

Analytical and bioanalytical chemistry
2012 | Journal article
Source: Self-asserted source
Huiwang Ai

Fluorescent biosensors for chemicals of environmental and biological interests

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
2012 | Conference paper
Source: Self-asserted source
Huiwang Ai

Reaction-based genetically encoded fluorescent hydrogen sulfide sensors

Journal of the American Chemical Society
2012 | Journal article
Source: Self-asserted source
Huiwang Ai

Forster resonance energy transfer-based biosensors for multiparameter ratiometric imaging of Ca2+ dynamics and caspase-3 activity in single cells

Analytical chemistry
2011 | Journal article
Source: Self-asserted source
Huiwang Ai

Probing Protein--Protein Interactions with a Genetically Encoded Photo-crosslinking Amino Acid

Chembiochem
2011 | Journal article
Source: Self-asserted source
Huiwang Ai

A method to site-specifically introduce methyllysine into proteins in E. coli

Chemical communications
2010 | Journal article
Source: Self-asserted source
Huiwang Ai

Genetically encoded alkenes in yeast

Angewandte Chemie International Edition
2010 | Journal article
Source: Self-asserted source
Huiwang Ai

Teal fluorescent proteins (mTFP1)

2010-09 | Other
Source: Self-asserted source
Huiwang Ai

Red fluorescent protein pH biosensor to detect concentrative nucleoside transport

Journal of Biological Chemistry
2009 | Journal article
Source: Self-asserted source
Huiwang Ai

Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors

Nature Methods
2008 | Journal article
Source: Self-asserted source
Huiwang Ai

Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging

BMC biology
2008 | Journal article
Source: Self-asserted source
Huiwang Ai

Identification of sites within a monomeric red fluorescent protein that tolerate peptide insertion and testing of corresponding circular permutations

Photochemistry and photobiology
2008 | Journal article
Source: Self-asserted source
Huiwang Ai
Items per page:
Page 1 of 2

Peer review (34 reviews for 16 publications/grants)

Review activity for Accounts of chemical research. (1)
Review activity for ACS central science. (1)
Review activity for ACS chemical biology. (5)
Review activity for ACS omega. (1)
Review activity for ACS sensors. (5)
Review activity for Analytical chemistry. (4)
Review activity for Biochemistry. (1)
Review activity for Bioconjugate chemistry. (2)
Review activity for ChemBioChem. (2)
Review activity for Chemical research in toxicology. (1)
Review activity for Chemical reviews. (1)
Review activity for Journal of medicinal chemistry. (1)
Review activity for Journal of organic chemistry. (1)
Review activity for Journal of the American Chemical Society. (6)
Review activity for Nature methods. (1)
Review activity for Organic letters. (1)
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