Personal information

United States

Biography

I am a molecular biologist (PhD) with 7 years of research experience from the Lourido Lab at the Whitehead Institute of MIT. In my research, I optimized and established CRISPR-based tools for gene-editing in the human parasite Toxoplasma gondii—whereby I also outlined some starting points for employing CRISPR interference. I also generated a genomic dataset of transcription initiation events, and defined the major transcription start sites for the majority (7603 or ~91%) of protein-coding genes. These data are available through my publication and the Toxoplasma Genome Database (ToxoDB). Please get in touch if you have any feedback or questions about my work.

Activities

Employment (1)

Whitehead Institute for Biomedical Research: Cambridge, MA, US

2020-04-01 to 2021-10-31 | Visiting Scientist
Employment
Source: Self-asserted source
Benedikt M. Markus

Education and qualifications (2)

Whitehead Institute for Biomedical Research: Cambridge, MA, US

2015-05-01 to 2020-03-31 | PhD
Education
Source: Self-asserted source
Benedikt M. Markus

University of Freiburg: Freiburg, Baden-Württemberg, DE

2008-10-01 to 2015-05-01 | Bachelor's and Master's
Education
Source: Self-asserted source
Benedikt M. Markus

Funding (1)

Exploring Mechanisms of Drug Resistance and Epistasis in Apicomplexan Parasites, Using Genome-Wide CRISPR-Mediated Control of Gene Expression

2015-08 to 2018-04 | Salary award
Boehringer Ingelheim Fonds (Mainz, DE)
Source: Self-asserted source
Benedikt M. Markus

Works (11)

Metabolic adaptability and nutrient scavenging in Toxoplasma gondii : insights from ingestion pathway-deficient mutants

mSphere
2025-04-02 | Journal article
Contributors: Patrick A. Rimple; Einar B. Olafsson; Benedikt M. Markus; Fengrong Wang; Leonardo Augusto; Sebastian Lourido; Vern B. Carruthers; Gustavo Arrizabalaga
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Crossref

Metabolic Adaptability and Nutrient Scavenging in Toxoplasma gondii: Insights from Ingestion Pathway-Deficient Mutants

2024-11-27 | Preprint
Contributors: Patrick A. Rimple; Einar B. Olafsson; Benedikt M. Markus; Fengrong Wang; Leonardo Augusto; Sebastian Lourido; Vern B. Carruthers
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High-Resolution Mapping of Transcription Initiation in the Asexual Stages of Toxoplasma gondii

Frontiers in Cellular and Infection Microbiology
2021 | Journal article
EID:

2-s2.0-85100598735

Part of ISSN: 22352988
Contributors: Markus, B.M.; Waldman, B.S.; Lorenzi, H.A.; Lourido, S.
Source: Self-asserted source
Benedikt M. Markus via Scopus - Elsevier

CRISPR-Mediated Transcriptional Repression in Toxoplasma gondii

mSphere
2021-10-27 | Journal article
Contributors: Benedikt M. Markus; Elizabeth A. Boydston; Sebastian Lourido; Ira J. Blader
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Crossref
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Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFNγ-activated murine macrophages

Nature Communications
2020-10-16 | Journal article
Contributors: Yifan Wang; Lamba Omar Sangaré; Tatiana C. Paredes-Santos; Musa A. Hassan; Shruthi Krishnamurthy; Anna M. Furuta; Benedikt M. Markus; Sebastian Lourido; Jeroen P. J. Saeij
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Genetic screens reveal a central role for heme metabolism in artemisinin susceptibility

Nature Communications
2020-09-23 | Journal article
Contributors: Clare R. Harding; Saima M. Sidik; Boryana Petrova; Nina F. Gnädig; John Okombo; Alice L. Herneisen; Kurt E. Ward; Benedikt M. Markus; Elizabeth A. Boydston; David A. Fidock et al.
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Identifying the Target of an Antiparasitic Compound in Toxoplasma Using Thermal Proteome Profiling.

ACS chemical biology
2020-07 | Journal article
Source: Self-asserted source
Benedikt M. Markus
grade
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A genome-wide loss-of-function screen identifiesToxoplasma gondiigenes that determine fitness in interferon gamma-activated murine macrophages

2019-12 | Preprint
OTHER-ID:

PPR104837

Contributors: Wang Y; Sangaré LO; Paredes-Santos TC; Krishnamurthy S; Hassan MA; Furuta AM; Markus BM; Lourido S; Saeij JP
Source: Self-asserted source
Benedikt M. Markus via Europe PubMed Central
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Genetic screens reveal a central role for heme biosynthesis in artemisinin susceptibility

2019-08 | Preprint
OTHER-ID:

PPR90303

Contributors: Harding CR; Sidik SM; Petrova B; Gnädig NF; Okombo J; Ward KE; Markus BM; Fidock DA; Lourido S
Source: Self-asserted source
Benedikt M. Markus via Europe PubMed Central
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Optimizing Systems for Cas9 Expression in Toxoplasma gondii

mSphere
2019-06-26 | Journal article
Contributors: Benedikt M. Markus; George W. Bell; Hernan A. Lorenzi; Sebastian Lourido; Ira J. Blader
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Allosteric activation of apicomplexan calcium-dependent protein kinases.

Proceedings of the National Academy of Sciences of the United States of America
2015-08 | Journal article
Contributors: Ingram JR; Knockenhauer KE; Markus BM; Mandelbaum J; Ramek A; Shan Y; Shaw DE; Schwartz TU; Ploegh HL; Lourido S
Source: Self-asserted source
Benedikt M. Markus via Europe PubMed Central
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