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Vrije Universiteit Brussel: Brussels, BE

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Works (50 of 51)

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A biosensor-based phage display selection method for automated, high-throughput Nanobody discovery

Biosensors and Bioelectronics
2025-03 | Journal article
DOI: 10.1016/j.bios.2024.116951
Contributors: Phebe De Keyser; Valentina Kalichuk; Thomas Zögg; Alexandre Wohlkönig; Stephan Schenck; Janine Brunner; Els Pardon; Jan Steyaert
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A nanobody-based microfluidic chip for fast and automated purification of protein complexes

Lab on a Chip
2024 | Journal article
DOI: 10.1039/D4LC00728J
Contributors: Phebe De Keyser; Mitch de Waard; Ignaas S. M. Jimidar; Sandrien Verloy; Steven Janvier; Valentina Kalichuk; Thomas Zögg; Alexandre Wohlkönig; Els Pardon; Jan Steyaert et al.
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Allosteric nanobodies to study the interactions between SOS1 and RAS

Nature Communications
2024-07-23 | Journal article | Author
DOI: 10.1038/s41467-024-50349-2
SOURCE-WORK-ID:

116d1398-20d3-49bf-ae79-a9f3a367793d

PMID: 39043660
EID:

2-s2.0-85199308968

WOSUID:

001275549300004
Contributors: Baptiste Fischer; Tomasz Uchański; Aidana Sheryazdanova; Simon Gonzalez; Alexander N Volkov; Elke Brosens; Thomas Zögg; Valentina V. Kalichuk; Steven Ballet; Wim Versées et al.
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Vrije Universiteit Brussel

Discovery and pharmacological characterization of nanobodies acting as potent positive allosteric modulators of the calcium-sensing receptor

2024-07-08 | Preprint
DOI: 10.1101/2024.07.08.602375
Contributors: Iris Mos; Thomas Zögg; Alexandre Wohlkönig; Anne Mette Helmich Egholm; Sabrina N. Rahman; Els Pardon; Jan Steyaert; Hans Bräuner-Osborne; Jesper M. Mathiesen
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A biosensor-based phage display selection method for automated, high-throughput Nanobody discovery

2024-06-26 | Preprint
DOI: 10.1101/2024.06.26.600780
Contributors: Phebe De Keyser; Valentina Kalichuk; Thomas Zögg; Alexandre Wohlkönig; Stephan Schenck; Janine Brunner; Els Pardon; Jan Steyaert
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Nanobody Loop Mimetics Enhance Son of Sevenless 1‐Catalyzed Nucleotide Exchange on RAS**

Angewandte Chemie International Edition
2023-06-12 | Journal article
DOI: 10.1002/anie.202219095
Contributors: Kevin Van holsbeeck; Baptiste Fischer; Simon Gonzalez; Charlène Gadais; Wim Versées; José C. Martins; Charlotte Martin; Alexandre Wohlkönig; Jan Steyaert; Steven Ballet
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Nanobody Loop Mimetics Enhance Son of Sevenless 1‐Catalyzed Nucleotide Exchange on RAS**

Angewandte Chemie
2023-06-12 | Journal article
DOI: 10.1002/ange.202219095
Contributors: Kevin Van holsbeeck; Baptiste Fischer; Simon Gonzalez; Charlène Gadais; Wim Versées; José C. Martins; Charlotte Martin; Alexandre Wohlkönig; Jan Steyaert; Steven Ballet
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Nanobody CDR3 mimetics enhance SOS1-catalyzed nucleotide exchange on RAS

2022-12-12 | Preprint
DOI: 10.1101/2022.12.09.519747
Contributors: Kevin Van holsbeeck; Baptiste Fischer; Simon Gonzalez; Charlène Gadais; Wim Versées; José C. Martins; Charlotte Martin; Alexandre Wohlkönig; Jan Steyaert; Steven Ballet
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Downsizing Nanobodies: Towards CDR3 Loop Mimetics Modulating Intracellular Protein-Protein Interactions

Proceedings of the 36th European and the 12th International Peptide Symposium
2022-11-04 | Conference paper | Author
DOI: 10.17952/36EPS.2022.141
SOURCE-WORK-ID:

846b1c40-61f7-49f2-a457-002c5ec9b2b2
Contributors: Kevin Van holsbeeck; Baptiste Fischer; Simon Gonzalez; Charlène Gadais; Wim Versées; José C. Martins; Charlotte Martin; Wohlkonig; Jan Steyaert; Steven Ballet
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Vrije Universiteit Brussel

Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM

Nature Methods
2021-01 | Journal article | Author
DOI: 10.1038/s41592-020-01001-6
SOURCE-WORK-ID:

ecc7ac52-bf79-404b-a10c-376958b75a75

PMID: 33408403
EID:

2-s2.0-85098856150

WOSUID:

000621860400026
Contributors: Tomasz Uchański; Simonas Masiulis; Baptiste Fischer; Valentina V. Kalichuk; Uriel López-Sánchez; Eleftherios Zarkadas; Miriam Weckener; Andrija Sente; Philip Ward; Wohlkonig et al.
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Vrije Universiteit Brussel

Constructing and purifying megabodies starting from individual nanobody sequences

2021-01-07 | Other
DOI: 10.21203/rs.3.pex-1033/v1
Contributors: Tomasz Uchański; Baptiste Fischer; Valentina Kalichuk; Alexandre Wohlkönig; Thomas Zögg; Els Pardon; Jan Steyaert
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Fragment-Based Optimized EthR Inhibitors with in Vivo Ethionamide Boosting Activity

ACS Infectious Diseases
2020-03-13 | Journal article | Author
DOI: 10.1021/acsinfecdis.9b00277
SOURCE-WORK-ID:

bfe9ace9-bb59-4e70-926f-60024ea8be55

PMID: 32011115
EID:

2-s2.0-85081944520

WOSUID:

000526415000004
Contributors: Baptiste Villemagne; Arnaud Machelart; Ngoc Chau Tran; Marion Flipo; Martin Moune; Florence Leroux; Catherine Piveteau; Wohlkonig; René Wintjens; Xue Li et al.
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Vrije Universiteit Brussel

Methyl arachidonyl fluorophosphonate inhibits Mycobacterium tuberculosis thioesterase TesA and globally affects vancomycin susceptibility

FEBS Letters
2020-01 | Journal article | Author
DOI: 10.1002/1873-3468.13555
SOURCE-WORK-ID:

d1caf54d-d60e-4b8f-ac39-1614817e04da

EID:

2-s2.0-85071325636

WOSUID:

000484027300001
Contributors: Dong Yang; Guy Vandenbussche; Didier Vertommen; Damien Evrard; Romany Abskharon; Jean‐François Cavalier; Gilles Berger; Stéphane Canaan; Mohammad Shahneawz Khan; Sheng Zeng et al.
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Vrije Universiteit Brussel

Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM

2019-10-22 | Other
DOI: 10.1101/812230
Contributors: Tomasz Uchański; Simonas Masiulis; Baptiste Fischer; Valentina Kalichuk; Alexandre Wohlkönig; Thomas Zögg; Han Remaut; Wim Vranken; A. Radu Aricescu; Els Pardon et al.
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A fragment-based approach towards the discovery of N-substituted tropinones as inhibitors of Mycobacterium tuberculosis transcriptional regulator EthR2

European Journal of Medicinal Chemistry
2019-04 | Journal article
DOI: 10.1016/j.ejmech.2019.02.023
Contributors: Hugues Prevet; Martin Moune; Abdalkarim Tanina; Christian Kemmer; Adrien Herledan; Rosangela Frita; Alexandre Wohlkönig; Marilyne Bourotte; Baptiste Villemagne; Florence Leroux et al.
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A comprehensive analysis of the protein-ligand interactions in crystal structures of Mycobacterium tuberculosis EthR

Biochimica et Biophysica Acta - Proteins and Proteomics
2019-03-01 | Journal article | Author
DOI: 10.1016/j.bbapap.2018.12.003
SOURCE-WORK-ID:

970f8edd-a1df-4e5c-91e9-1c8435e6e267

EID:

2-s2.0-85059696479

WOSUID:

000457507600010
Contributors: Abdalkarim Tanina; Wohlkonig; Sameh H. Soror; Marion Flipo; Baptiste Villemagne; Hugues Prevet; Benoit Déprez; Martin Moune; Hélène Perée; Franck Meyer et al.
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Vrije Universiteit Brussel

An improved yeast surface display platform for the screening of nanobody immune libraries

Scientific Reports
2019-01-23 | Journal article
DOI: 10.1038/s41598-018-37212-3
Contributors: Tomasz Uchański; Thomas Zögg; Jie Yin; Daopeng Yuan; Alexandre Wohlkönig; Baptiste Fischer; Daniel M. Rosenbaum; Brian K. Kobilka; Els Pardon; Jan Steyaert
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Correction to Discovery, Structure-Activity Relationship, and Binding Mode of an Imidazo[1,2- a]pyridine Series of Autotaxin Inhibitors.

Journal of medicinal chemistry
2018-04 | Journal article
DOI: 10.1021/acs.jmedchem.8b00579
PMID: 29672037
Contributors: Joncour A; Desroy N; Housseman C; Bock X; Bienvenu N; Cherel L; Labeguere V; Peixoto C; Annoot D; Lepissier L et al.
Source: Self-asserted source
Wohlkonig via Europe PubMed Central

Soluble polymorphic bank vole prion proteins induced by co-expression of quiescin sulfhydryl oxidase in E. coli and their aggregation behaviors.

Microbial cell factories
2017-10 | Journal article
DOI: 10.1186/s12934-017-0782-x
PMID: 28978309
PMC: PMC5628483
Contributors: Abskharon R; Dang J; Elfarash A; Wang Z; Shen P; Zou LS; Hassan S; Wang F; Fujioka H; Steyaert J et al.
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Wohlkonig via Europe PubMed Central

Discovery, Structure-Activity Relationship, and Binding Mode of an Imidazo[1,2-a]pyridine Series of Autotaxin Inhibitors.

Journal of medicinal chemistry
2017-08 | Journal article
DOI: 10.1021/acs.jmedchem.7b00647
PMID: 28731719
Contributors: Joncour A; Desroy N; Housseman C; Bock X; Bienvenu N; Cherel L; Labeguere V; Peixoto C; Annoot D; Lepissier L et al.
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Wohlkonig via Europe PubMed Central

Structural analysis of the interaction between spiroisoxazoline SMARt-420 and the Mycobacterium tuberculosis repressor EthR2

Biochemical and Biophysical Research Communications
2017-05 | Journal article
DOI: 10.1016/j.bbrc.2017.04.074
Contributors: Alexandre Wohlkönig; Han Remaut; Martin Moune; Abdalkarim Tanina; Franck Meyer; Matthieu Desroses; Jan Steyaert; Nicolas Willand; Alain R. Baulard; René Wintjens
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Reversion of antibiotic resistance in Mycobacterium tuberculosis by spiroisoxazoline SMARt-420.

Science (New York, N.Y.)
2017-03 | Journal article
DOI: 10.1126/science.aag1006
PMID: 28302858
Contributors: Blondiaux N; Moune M; Desroses M; Frita R; Flipo M; Mathys V; Soetaert K; Kiass M; Delorme V; Djaout K et al.
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Wohlkonig via Europe PubMed Central
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Quiescin-sulfhydryl oxidase inhibits prion formation in vitro.

Aging
2016-12 | Journal article
DOI: 10.18632/aging.101132
PMID: 27959866
PMC: PMC5270677
Contributors: Zhan YA; Abskharon R; Li Y; Yuan J; Zeng L; Dang J; Martinez MC; Wang Z; Mikol J; Lehmann S et al.
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Wohlkonig via Europe PubMed Central

Crystallization and initial crystallographic analysis of covalent DNA-cleavage complexes of Staphyloccocus aureus DNA gyrase with QPT-1, moxifloxacin and etoposide.

2015-10 | Journal article
DOI: 10.1107/S2053230X15015290
PMID: 26457513
Contributors: Srikannathasan V; Wohlkonig A; Shillings A; Singh O; Chan PF; Huang J; Gwynn MN; Fosberry AP; Homes P; Hibbs M et al.
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Wohlkonig via Europe PubMed Central
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Production, crystallization and preliminary X-ray diffraction of the Gαs α-helical domain in complex with a nanobody.

2014-11 | Journal article
DOI: 10.1107/S2053230X14020962
PMID: 25372817
Contributors: Triest S; Wohlkönig A; Pardon E; Steyaert J
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Wohlkonig via Europe PubMed Central
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Ligand efficiency driven design of new inhibitors of Mycobacterium tuberculosis transcriptional repressor EthR using fragment growing, merging, and linking approaches.

2014-06 | Journal article
DOI: 10.1021/jm500422b
PMID: 24818704
Contributors: Villemagne B; Flipo M; Blondiaux N; Crauste C; Malaquin S; Leroux F; Piveteau C; Villeret V; Brodin P; Villoutreix BO et al.
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Wohlkonig via Europe PubMed Central

The Mycobacterium tuberculosis transcriptional repressor EthR is negatively regulated by Serine/Threonine phosphorylation

Biochemical and Biophysical Research Communications
2014-04 | Journal article
DOI: 10.1016/j.bbrc.2014.03.074
Contributors: Jade Leiba; Séverine Carrère-Kremer; Nicolas Blondiaux; Martin Moune Dimala; Alexandre Wohlkönig; Alain Baulard; Laurent Kremer; Virginie Molle
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A general protocol for the generation of Nanobodies for structural biology.

2014-03 | Journal article
DOI: 10.1038/nprot.2014.039
PMID: 24577359
PMC: PMC4297639
Contributors: Pardon E; Laeremans T; Triest S; Rasmussen SG; Wohlkönig A; Ruf A; Muyldermans S; Hol WG; Kobilka BK; Steyaert J
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Wohlkonig via Europe PubMed Central

Probing the N-terminal β-sheet conversion in the crystal structure of the human prion protein bound to a nanobody.

2014-01 | Journal article
DOI: 10.1021/ja407527p
PMID: 24400836
Contributors: Abskharon RN; Giachin G; Wohlkonig A; Soror SH; Pardon E; Legname G; Steyaert J
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Wohlkonig via Europe PubMed Central

Characterization of the PAS domain in the sensor-kinase BvgS: mechanical role in signal transmission.

2013 | Journal article
DOI: 10.1186/1471-2180-13-172
PMID: 23883404
PMC: PMC3726324
Contributors: Dupré E; Wohlkonig A; Herrou J; Locht C; Jacob-Dubuisson F; Antoine R
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Wohlkonig via Europe PubMed Central

Recombinant human prion protein inhibits prion propagation in vitro.

2013 | Journal article
DOI: 10.1038/srep02911
PMID: 24105336
PMC: PMC3793212
Contributors: Yuan J; Zhan YA; Abskharon R; Xiao X; Martinez MC; Zhou X; Kneale G; Mikol J; Lehmann S; Surewicz WK et al.
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Wohlkonig via Europe PubMed Central

High-resolution structure of a papaya plant-defense barwin-like protein solved by in-house sulfur-SAD phasing.

2013-10 | Journal article
DOI: 10.1107/S0907444913018015
PMID: 24100320
Contributors: Huet J; Teinkela Mbosso EJ; Soror S; Meyer F; Looze Y; Wintjens R; Wohlkönig A
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Wohlkonig via Europe PubMed Central
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The mannose-specific lectin domains of Flo1p from Saccharomyces cerevisiae and Lg-Flo1p from S. pastorianus: crystallization and preliminary X-ray diffraction analysis of the adhesin-carbohydrate complexes.

2013-07 | Journal article
DOI: 10.1107/S1744309113015030
PMID: 23832207
PMC: PMC3702324
Contributors: Ielasi FS; Goyal P; Sleutel M; Wohlkonig A; Willaert RG
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Wohlkonig via Europe PubMed Central

Expression, purification, characterization, and solution nuclear magnetic resonance study of highly deuterated yeast cytochrome C peroxidase with enhanced solubility.

2013-04 | Journal article
DOI: 10.1021/bi400220w
PMID: 23517193
Contributors: Volkov AN; Wohlkonig A; Soror SH; van Nuland NA
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Wohlkonig via Europe PubMed Central
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A novel expression system for production of soluble prion proteins in E. coli.

2012 | Journal article
DOI: 10.1186/1475-2859-11-6
PMID: 22233534
PMC: PMC3283519
Contributors: Abskharon RN; Ramboarina S; El Hassan H; Gad W; Apostol MI; Giachin G; Legname G; Steyaert J; Messens J; Soror SH et al.
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Wohlkonig via Europe PubMed Central

Discovery of novel N-phenylphenoxyacetamide derivatives as EthR inhibitors and ethionamide boosters by combining high-throughput screening and synthesis.

2012-07 | Journal article
DOI: 10.1021/jm300377g
PMID: 22738293
Contributors: Flipo M; Willand N; Lecat-Guillet N; Hounsou C; Desroses M; Leroux F; Lens Z; Villeret V; Wohlkönig A; Wintjens R et al.
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Wohlkonig via Europe PubMed Central
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Ethionamide boosters. 2. Combining bioisosteric replacement and structure-based drug design to solve pharmacokinetic issues in a series of potent 1,2,4-oxadiazole EthR inhibitors.

Journal of medicinal chemistry
2011-12 | Journal article
DOI: 10.1021/jm200825u
PMID: 22098589
Contributors: Flipo M; Desroses M; Lecat-Guillet N; Villemagne B; Blondiaux N; Leroux F; Piveteau C; Mathys V; Flament MP; Siepmann J et al.
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Wohlkonig via Europe PubMed Central
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Structural activation of the transcriptional repressor EthR from Mycobacterium tuberculosis by single amino acid change mimicking natural and synthetic ligands.

Nucleic acids research
2011-12 | Journal article
DOI: 10.1093/nar/gkr1113
PMID: 22156370
PMC: PMC3326297
Contributors: Carette X; Blondiaux N; Willery E; Hoos S; Lecat-Guillet N; Lens Z; Wohlkönig A; Wintjens R; Soror SH; Frénois F et al.
Source: Self-asserted source
Wohlkonig via Europe PubMed Central

Combining in-situ proteolysis and microseed matrix screening to promote crystallization of PrPc-nanobody complexes.

Protein engineering, design & selection : PEDS
2011-05 | Journal article
DOI: 10.1093/protein/gzr017
PMID: 21536542
Contributors: Abskharon RN; Soror SH; Pardon E; El Hassan H; Legname G; Steyaert J; Wohlkonig A
Source: Self-asserted source
Wohlkonig via Europe PubMed Central

Crystallization and preliminary X-ray diffraction analysis of kanamycin-binding β-lactamase in complex with its ligand.

Acta crystallographica. Section F, Structural biology and crystallization communications
2011-05 | Journal article
DOI: 10.1107/S1744309111013832
PMID: 21636917
PMC: PMC3107148
Contributors: Van de Water K; Soror SH; Wohlkonig A; van Nuland NA; Volkov AN
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Wohlkonig via Europe PubMed Central
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Crystallization and preliminary X-ray diffraction analysis of a specific VHH domain against mouse prion protein.

Acta crystallographica. Section F, Structural biology and crystallization communications
2010-11 | Journal article
DOI: 10.1107/S1744309110042168
PMID: 21139215
PMC: PMC2998374
Contributors: Abskharon RN; Soror SH; Pardon E; El Hassan H; Legname G; Steyaert J; Wohlkonig A
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Wohlkonig via Europe PubMed Central

Structural relationships in the lysozyme superfamily: significant evidence for glycoside hydrolase signature motifs.

PloS one
2010-11 | Journal article
DOI: 10.1371/journal.pone.0015388
PMID: 21085702
PMC: PMC2976769
Contributors: Wohlkönig A; Huet J; Looze Y; Wintjens R
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Wohlkonig via Europe PubMed Central
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Structural basis of quinolone inhibition of type IIA topoisomerases and target-mediated resistance.

Nature structural & molecular biology
2010-08 | Journal article
DOI: 10.1038/nsmb.1892
PMID: 20802486
Contributors: Wohlkonig A; Chan PF; Fosberry AP; Homes P; Huang J; Kranz M; Leydon VR; Miles TJ; Pearson ND; Perera RL et al.
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Type IIA topoisomerase inhibition by a new class of antibacterial agents.

Nature
2010-08 | Journal article
DOI: 10.1038/nature09197
PMID: 20686482
Contributors: Bax BD; Chan PF; Eggleston DS; Fosberry A; Gentry DR; Gorrec F; Giordano I; Hann MM; Hennessy A; Hibbs M et al.
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Wohlkonig via Europe PubMed Central
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Structure and plasticity of the peptidyl-prolyl isomerase Par27 of Bordetella pertussis revealed by X-ray diffraction and small-angle X-ray scattering.

Journal of structural biology
2009-11 | Journal article
DOI: 10.1016/j.jsb.2009.11.007
PMID: 19932182
Contributors: Clantin B; Leyrat C; Wohlkönig A; Hodak H; Ribeiro Ede A; Martinez N; Baud C; Smet-Nocca C; Villeret V; Jacob-Dubuisson F et al.
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Wohlkonig via Europe PubMed Central

Crystallization and preliminary X-ray diffraction analysis of the peptidylprolyl isomerase Par27 of Bordetella pertussis.

Acta crystallographica. Section F, Structural biology and crystallization communications
2008-08 | Journal article
DOI: 10.1107/S1744309108024731
PMID: 18765910
PMC: PMC2531267
Contributors: Wohlkönig A; Hodak H; Clantin B; Sénéchal M; Bompard C; Jacob-Dubuisson F; Villeret V
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Wohlkonig via Europe PubMed Central

The peptidyl-prolyl isomerase and chaperone Par27 of Bordetella pertussis as the prototype for a new group of parvulins.

Journal of molecular biology
2007-11 | Journal article
DOI: 10.1016/j.jmb.2007.10.088
PMID: 18164725
Contributors: Hodak H; Wohlkönig A; Smet-Nocca C; Drobecq H; Wieruszeski JM; Sénéchal M; Landrieu I; Locht C; Jamin M; Jacob-Dubuisson F
Source: Self-asserted source
Wohlkonig via Europe PubMed Central

Expression and purification in high yield of a functionally active recombinant human Type I inositol(1,4,5)P3 5-phosphatase.

Protein expression and purification
2007-04 | Journal article
DOI: 10.1016/j.pep.2007.04.009
PMID: 17537645
Contributors: Wohlkönig A; Sénéchal M; Dewitte F; Backers K; Erneux C; Villeret V
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Wohlkonig via Europe PubMed Central

[Multiple post-translational modifications implied in the gene expression regulation: example of the sumoylation of Ets factors]

Bulletin et memoires de l'Academie royale de medecine de Belgique
2007-01 | Journal article
PMID: 18404999
Contributors: de Launoit Y; Degerny C; Wohlkonig A; Baert JL
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Wohlkonig via Europe PubMed Central
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Biphenyl 2,3',4,5',6-pentakisphosphate, a novel inositol polyphosphate surrogate, modulates Ca2+ responses in rat hepatocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology
2007-01 | Journal article
DOI: 10.1096/fj.06-7691com
PMID: 17264160
Contributors: Vandeput F; Combettes L; Mills SJ; Backers K; Wohlkönig A; Parys JB; De Smedt H; Missiaen L; Dupont G; Potter BV et al.
Source: Self-asserted source
Wohlkonig via Europe PubMed Central
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