Tiziana Bonaldi is Group Leader at the Department of Experimental Oncology of the European Institute of Oncology, where she has been directing the Giovanni Armenise-Harvard Foundation Laboratory on “Quantitative proteomics to study regulation of gene expression through chromatin structure and dynamics” since 2008.
Following his training in Biology at the University of Milan, she obtained her PhD degree in Molecular and Cellular Biology at DiBit- San Raffaele Scientific Institute in Milan (Italy), working in the laboratory of chromatin dynamics under the supervision of Prof. Marco E. Bianchi.
In 2003, he was awarded a long-term EMBO fellowship and joined as post-doctoral fellow the laboratory of Prof. Axel Imhof at the Department of Molecular Biology Dpt. of the Ludwig Maximillians University of Munich (Germany), headed by Prof. Peter B. Becker. During this first post-doc TB pioneered the use MS for the characterisation of complex patterns of histone PTMs in various loci of the genome, in relation to different functional states of the underlying chromatin (Bonaldi et al., 2004a and b; Loyola and Bonaldi, 2006).
With a second post-doctoral research, carried out in the Department of Proteomics and Signal Transduction headed by Prof. Matthias Mann at the Max Planck Institute of Biochemistry in Martinsried (Germany), she focused on chromatin and post-transcriptional regulation of gene expression using cutting-edge technology in proteomics and mass spectrometry. She published a groundbreaking study, demonstrating the power of proteomics to investigate gene expression regulatory events (Bonaldi et al, 2008).
Since 2008, she returned to Italy to start her independent research, working as Group Leader at IEO, where she strengthened her expertise in the application of Proteomics to study multi-layered mechanism of gene expression regulation, establishing innovative studies for:
• The analysis of Chromatin composition in a locus –specific manner (Soldi and Bonaldi, 2013);
• The Accurate profiling of hPTMS by MS, both from cultured cell lines and primary specimens (cells, fresh frozen and FFPE-tissues) (Noberini et al. 2015);
• The systematic and quantitative analysis of the methyl-proteome (Bremang et al., 2013);
• Drug targets deconvolution screening by chemo-proteomics (Colzani et al, 2014)
• The comprehensive analysis of microRNA effect on gene expression during cancer progression (Mihailovich et al., 2015)
TB research is performed both independently and in collaboration with several members of the Italian and European epigenetic and chromatin network. Her research has been positively evaluated by various granting agencies, with, 12 distinct national and international, peer-reviewed research project grants, of which 9 as PI, since 2008.
In 2007 she was awarded the “Armenise-Harvard Career Development Award” to support her transition to independence and in 2010 she was awarded the “International Inner Wheel for Women” for her scientific achievements. In 2014 she successfully completed her tenure period and was appointed as tenured group leader at IEO, with a position of Associate Professor.