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Employment (2)

Texas A&M University: College Station, Texas, US

2019 to present | Assistant Professor (Biomedical Engineering)
Employment
Source: Self-asserted source
Alex J. Walsh

Morgridge Institute for Research: Madison, WI, US

2017 to 2019 | Assistant Scientist (Medical Engineering)
Employment
Source: Self-asserted source
Alex J. Walsh

Education and qualifications (4)

Air Force Research Laboratory: JBSA, Texas, US

2017 | NRC Post-doc (Optical Radiation/Bioeffects)
Education
Source: Self-asserted source
Alex J. Walsh

Vanderbilt University: Nashville, TN, US

2015 | PhD (Biomedical Engineering)
Education
Source: Self-asserted source
Alex J. Walsh

Vanderbilt University: Nashville, TN, US

2012 | MS (Biomedical Engineering)
Education
Source: Self-asserted source
Alex J. Walsh

Vanderbilt University: Nashville, Tennessee, US

2010 | BE (Biomedical Engineering)
Education
Source: Self-asserted source
Alex J. Walsh

Funding (5)

Equipment to accelerate studies of the effects of electromagnetic radiation on living organisms

2023-09-01 to 2024-08-31 | Grant
United States Department of the Air Force (Washington D.C., US)
URL: https://app.dimensions.ai/details/grant/grant.14525585
GRANT_NUMBER:

FA95502310537
Source: Self-asserted source
Alex J. Walsh via DimensionsWizard

Controlled Manipulation of Cell Function via Infrared Light

2023-08-15 to 2027-08-14 | Grant
United States Department of the Air Force (Washington D.C., US)
URL: https://app.dimensions.ai/details/grant/grant.14525491
GRANT_NUMBER:

FA95502310441
Source: Self-asserted source
Alex J. Walsh via DimensionsWizard

Autofluorescence lifetime microscopy for label-free detection of cell metabolism for cell biology research

2021-09-23 to 2026-06-30 | Grant
National Institute of General Medical Sciences (Bethesda, US)
URL: https://app.dimensions.ai/details/grant/grant.9847551
GRANT_NUMBER: R35GM142990
Source: Self-asserted source
Alex J. Walsh via DimensionsWizard

Light-Sheet Microscope with Optical Modulation Unit for Research on Biophysical Effects of Infrared Radiation

2021-07-01 to 2022-06-30 | Grant
United States Department of the Air Force (Washington D.C., US)
URL: https://app.dimensions.ai/details/grant/grant.13282942
GRANT_NUMBER:

FA95502110280
Source: Self-asserted source
Alex J. Walsh via DimensionsWizard

OPTICAL MODULATION OF CELL REDOX STATE

2020-05-01 to 2023-04-30 | Grant
United States Air Force (Arlington, US)
URL: https://app.dimensions.ai/details/grant/grant.9764257
GRANT_NUMBER:

FA95502010078
Source: Self-asserted source
Alex J. Walsh via DimensionsWizard

Works (50 of 56)

Items per page:
Page 1 of 2

Fluorescence lifetime imaging microscopy for metabolic analysis of LDHB inhibition in triple negative breast cancer

2025-01-19 | Preprint
DOI: 10.1101/2025.01.13.632864
Contributors: A. Galloway; B. Ter Hofstede; Alex J. Walsh
Source: check_circle
Crossref

Propofol Treatment Alters the Metabolic State of MDA-MB-231 Breast Cancer Cells

2025-01-19 | Preprint
DOI: 10.1101/2025.01.15.633222
Contributors: Tasbassum Ahmed Tasmi; Shubha Holla; Alex J. Walsh
Source: check_circle
Crossref

Timelapse and volumetric imaging of mitochondrial networking using NAD(P)H autofluorescence via 2-photon microscopy

2025-01-19 | Preprint
DOI: 10.1101/2025.01.15.633075
Contributors: Blanche ter Hofstede; Alex J. Walsh
Source: check_circle
Crossref

Label-free Detection and Characterization of Metabolism in Fresh and Cryopreserved Macrophages

2025-01-17 | Preprint
DOI: 10.1101/2025.01.13.632815
Contributors: Daniela De Hoyos Canales; Linghao Hu; Alex J. Walsh
Source: check_circle
Crossref

Optical metabolic imaging identifies metabolic shifts and mitochondria heterogeneity in POLG mutator macrophages

2025-01-15 | Preprint
DOI: 10.1101/2025.01.13.632822
Contributors: Linghao Hu; A. Phillip West; Alex J. Walsh
Source: check_circle
Crossref

Polarization light-sheet microscopy and tomography (PLµTo) for flow-based imaging of 3D microcarrier mesenchymal stem cell culture

2025-01-03 | Preprint
DOI: 10.1101/2025.01.02.631120
Contributors: Oscar R. Benavides; Berkley P. White; Roland Kaunas; Carl A. Gregory; Alex J. Walsh
Source: check_circle
Crossref

Fast autofluorescence imaging to evaluate dynamic changes in cell metabolism

Journal of Biomedical Optics
2024-12-19 | Journal article
DOI: 10.1117/1.jbo.29.12.126501
Part of ISSN: 1083-3668
Contributors: Anna Theodossiou; Jocelyn Martinez; Alex J. Walsh
Source: Self-asserted source
Alex J. Walsh

Evaluation of Cellpose segmentation with sequential thresholding for instance segmentation of cytoplasms within autofluorescence images

Computers in Biology and Medicine
2024-09 | Journal article
DOI: 10.1016/j.compbiomed.2024.108846
Contributors: Nianchao Wang; Linghao Hu; Alex J. Walsh
Source: check_circle
Crossref

Autofluorescence is a biomarker of neural stem cell activation state

Cell Stem Cell
2024-04 | Journal article
DOI: 10.1016/j.stem.2024.02.011
Part of ISSN: 1934-5909
Contributors: Christopher S. Morrow; Kelsey Tweed; Sabina Farhadova; Alex J. Walsh; Bo P. Lear; Avtar Roopra; Ryan D. Risgaard; Payton C. Klosa; Zachary P. Arndt; Ella R. Peterson et al.
Source: Self-asserted source
Alex J. Walsh

3D convolutional neural networks predict cellular metabolic pathway use from fluorescence lifetime decay data

APL Bioengineering
2024-03-01 | Journal article
DOI: 10.1063/5.0188476
Contributors: Linghao Hu; Daniela De Hoyos; Yuanjiu Lei; A. Phillip West; Alex J. Walsh
Source: check_circle
Crossref

Osr1 Regulates Macrophage-mediated Liver Inflammation in Nonalcoholic Fatty Liver Disease Progression

Cellular and Molecular Gastroenterology and Hepatology
2023 | Journal article
DOI: 10.1016/j.jcmgh.2022.12.010
Part of ISSN: 2352-345X
Contributors: Lin Liu; Yi Zhou; Zhimin Liu; Jiangyuan Li; Linghao Hu; Leya He; Guannan Gao; Brian Kidd; Alexandra Walsh; Rulang Jiang et al.
Source: Self-asserted source
Alex J. Walsh

Perfusability and immunogenicity of implantable pre-vascularized tissues recapitulating features of native capillary network

Bioactive Materials
2023-12 | Journal article
DOI: 10.1016/j.bioactmat.2023.07.023
Contributors: Dhavan Sharma; Archita Sharma; Linghao Hu; Te-An Chen; Sarah Voon; Kayla J. Bayless; Jeremy Goldman; Alex J. Walsh; Feng Zhao
Source: check_circle
Crossref

Label-free spatially maintained measurements of metabolic phenotypes in cells

Frontiers in Bioengineering and Biotechnology
2023-11-28 | Journal article
DOI: 10.3389/fbioe.2023.1293268
Part of ISSN: 2296-4185
Contributors: Linghao Hu; Nianchao Wang; Joshua D. Bryant; Lin Liu; Linglin Xie; A. Phillip West; Alex J. Walsh
Source: Self-asserted source
Alex J. Walsh

Comparison of polystyrene and hydrogel microcarriers for optical imaging of adherent cells

2023-10-13 | Preprint
DOI: 10.1101/2023.10.12.559796
Contributors: Oscar R. Benavides; Berkley P. White; Holly C. Gibbs; Roland Kaunas; Carl A. Gregory; Kristen C. Maitland; Alex J. Walsh
Source: check_circle
Crossref

Comparison of phasor analysis and biexponential decay curve fitting of autofluorescence lifetime imaging data for machine learning prediction of cellular phenotypes

Frontiers in Bioinformatics
2023-06-29 | Journal article
DOI: 10.3389/fbinf.2023.1210157
Part of ISSN: 2673-7647
Contributors: Linghao Hu; Blanche Ter Hofstede; Dhavan Sharma; Feng Zhao; Alex J. Walsh
Source: Self-asserted source
Alex J. Walsh

Evolution of cisplatin resistance through coordinated metabolic reprogramming of the cellular reductive state

British Journal of Cancer
2023-04-03 | Journal article
DOI: 10.1038/s41416-023-02253-7
Part of ISSN: 0007-0920
Part of ISSN: 1532-1827
Contributors: Wangie Yu; Yunyun Chen; Nagireddy Putluri; Abdullah Osman; Cristian Coarfa; Vasanta Putluri; Abu H. M. Kamal; Jennifer Kay Asmussen; Panagiotis Katsonis; Jeffrey N. Myers et al.
Source: Self-asserted source
Alex J. Walsh

Label-free optical imaging of cell function and collagen structure for cell-based therapies

Current Opinion in Biomedical Engineering
2023-03 | Journal article
DOI: 10.1016/j.cobme.2022.100433
Contributors: Linghao Hu; Samantha Morganti; Uyen Nguyen; Oscar R. Benavides; Alex J. Walsh
Source: check_circle
Crossref

POSEA: A novel algorithm to evaluate the performance of multi-object instance image segmentation

PLOS ONE
2023-03-29 | Journal article
DOI: 10.1371/journal.pone.0283692
Contributors: Nianchao Wang; Vijayalakshmi G. V. Mahesh; Linghao Hu; Alex J. Walsh
Source: check_circle
Crossref

Volumetric imaging of human mesenchymal stem cells (hMSCs) for non-destructive quantification of 3D cell culture growth

PLOS ONE
2023-03-28 | Journal article
DOI: 10.1371/journal.pone.0282298
Contributors: Oscar R. Benavides; Kun Chen; Holly C. Gibbs; Berkley P. White; Roland Kaunas; Carl A. Gregory; Alex J. Walsh; Kristen C. Maitland
Source: check_circle
Crossref

Decellularized organ biomatrices facilitate quantifiable <i>in vitro</i> 3D cancer metastasis models

Soft Matter
2022 | Journal article
DOI: 10.1039/d1sm01796a
Part of ISSN: 1744-683X
Part of ISSN: 1744-6848
Contributors: Sabrina N. VandenHeuvel; Heather A. Farris; Dillon A. Noltensmeyer; Sanjana Roy; Del A. Donehoo; Scott Kopetz; Svasti Haricharan; Alex J. Walsh; Shreya Raghavan
Source: Self-asserted source
Alex J. Walsh

Imaging in Scaffolds

Biomaterial Based Approaches to Study the Tumour Microenvironment
2022-12-07 | Book chapter
DOI: 10.1039/9781839166013-00304
Part of ISBN: 9781839162848
Part of ISBN: 9781839162848
Part of ISBN: 9781839166020
Part of ISBN: 9781839166013
Contributors: Jocelyn Martinez; Nianchao Wang; Linghao Hu; Elizabeth Cardona Benitez; Uyen Nguyen; Ricardo Martinez; Alex J. Walsh
Source: Self-asserted source
Alex J. Walsh

Design and Implementation of Privilege for Sale, a JEDI Activity for a Biomedical Engineering Introductory Course

Biomedical Engineering Education
2022-09 | Journal article
DOI: 10.1007/s43683-022-00070-7
Part of ISSN: 2730-5937
Part of ISSN: 2730-5945
Contributors: Alex J. Walsh; Shreya Raghavan
Source: Self-asserted source
Alex J. Walsh

Identification of rare cell populations in autofluorescence lifetime image data

Cytometry Part A
2022-06 | Journal article
DOI: 10.1002/cyto.a.24534
Contributors: Elizabeth N. Cardona; Alex J. Walsh
Source: check_circle
Crossref

In vivo fluorescence lifetime imaging of macrophage intracellular metabolism during wound responses in zebrafish

eLife
2022-02-24 | Journal article | Author
DOI: 10.7554/elife.66080
Part of ISSN: 2050-084X
Contributors: Veronika Miskolci; Kelsey E Tweed; Michael R Lasarev; Emily C Britt; Alex J. Walsh; Landon J Zimmerman; Courtney E McDougal; Mark R Cronan; Jing Fan; John-Demian Sauer et al.
Source: Self-asserted source
Alex J. Walsh

Autofluorescence Imaging to Evaluate Cellular Metabolism

Journal of Visualized Experiments
2021-11-15 | Journal article
DOI: 10.3791/63282
Part of ISSN: 1940-087X
Contributors: Anna Theodossiou; Linghao Hu; Nianchao Wang; Uyen Nguyen; Alex J. Walsh
Source: Self-asserted source
Alex J. Walsh

Extracellular pH affects the fluorescence lifetimes of metabolic co-factors

Journal of Biomedical Optics
2021-05-25 | Journal article
DOI: 10.1117/1.jbo.26.5.056502
Part of ISSN: 1083-3668
Source: Self-asserted source
Alex J. Walsh

Carbomer-based adjuvant elicits CD8 T-cell immunity by inducing a distinct metabolic state in cross-presenting dendritic cells

PLOS Pathogens
2021-01-14 | Journal article
DOI: 10.1371/journal.ppat.1009168
Part of ISSN: 1553-7374
Source: Self-asserted source
Alex J. Walsh

Fluorescence intensity and lifetime redox ratios detect metabolic perturbations in T cells

Biomedical Optics Express
2020-10-01 | Journal article
DOI: 10.1364/BOE.401935
Contributors: Linghao Hu; Nianchao Wang; Elizabeth Cardona; Alex J. Walsh
Source: check_circle
Crossref

Classification of T-cell activation via autofluorescence lifetime imaging

Nature Biomedical Engineering
2020-07-27 | Journal article
DOI: 10.1038/s41551-020-0592-z
Contributors: Alex J. Walsh; Katherine P. Mueller; Kelsey Tweed; Isabel Jones; Christine M. Walsh; Nicole J. Piscopo; Natalie M. Niemi; David J. Pagliarini; Krishanu Saha; Melissa C. Skala
Source: check_circle
Crossref

Classifying T cell activity in autofluorescence intensity images with convolutional neural networks

2019-08-15 | Other
DOI: 10.1101/737346
Contributors: Zijie J. Wang; Alex J. Walsh; Melissa C. Skala; Anthony Gitter
Source: check_circle
Crossref
grade
Preferred source (of 3)‎

Mutant KRAS Exosomes Alter the Metabolic State of Recipient Colonic Epithelial Cells

Cellular and Molecular Gastroenterology and Hepatology
2018 | Journal article
DOI: 10.1016/j.jcmgh.2018.01.013
Part of ISSN: 2352-345X
Source: Self-asserted source
Alex J. Walsh

Autofluorescence imaging identifies tumor cell-cycle status on a single-cell level

Journal of Biophotonics
2018-01 | Journal article
DOI: 10.1002/jbio.201600276
Part of ISSN: 1864-063X
Source: Self-asserted source
Alex J. Walsh

Functional Optical Imaging of Primary Human Tumor Organoids: Development of a Personalized Drug Screen

Journal of Nuclear Medicine
2017-09 | Journal article
DOI: 10.2967/jnumed.117.192534
Part of ISSN: 0161-5505
Part of ISSN: 2159-662X
Source: Self-asserted source
Alex J. Walsh

Action potential block in neurons by infrared light

Neurophotonics
2016-12-01 | Journal article
DOI: 10.1117/1.nph.3.4.040501
Part of ISSN: 2329-423X
Source: Self-asserted source
Alex J. Walsh

Optical Imaging of Drug-Induced Metabolism Changes in Murine and Human Pancreatic Cancer Organoids Reveals Heterogeneous Drug Response

Pancreas
2016-07 | Journal article
DOI: 10.1097/mpa.0000000000000543
Part of ISSN: 0885-3177
Source: Self-asserted source
Alex J. Walsh

Drug response in organoids generated from frozen primary tumor tissues

Scientific Reports
2016-05 | Journal article
DOI: 10.1038/srep18889
Part of ISSN: 2045-2322
Source: Self-asserted source
Alex J. Walsh

Temporal binning of time-correlated single photon counting data improves exponential decay fits and imaging speed

Biomedical Optics Express
2016-04-01 | Journal article
DOI: 10.1364/boe.7.001385
Part of ISSN: 2156-7085
Part of ISSN: 2156-7085
Source: Self-asserted source
Alex J. Walsh

In Vivo Autofluorescence Imaging of Tumor Heterogeneity in Response to Treatment

Neoplasia
2015-12 | Journal article
DOI: 10.1016/j.neo.2015.11.006
Part of ISSN: 1476-5586
Source: Self-asserted source
Alex J. Walsh

Signal Transducer and Activator of Transcription 3, Mediated Remodeling of the Tumor Microenvironment Results in Enhanced Tumor Drug Delivery in a Mouse Model of Pancreatic Cancer

Gastroenterology
2015-12 | Journal article
DOI: 10.1053/j.gastro.2015.07.058
Part of ISSN: 0016-5085
Source: Self-asserted source
Alex J. Walsh

Deconvolution of fluorescence lifetime imaging microscopy by a library of exponentials

Optics Express
2015-09-07 | Journal article
DOI: 10.1364/oe.23.023748
Part of ISSN: 1094-4087
Source: Self-asserted source
Alex J. Walsh

Blind deconvolution estimation of fluorescence measurements through quadratic programming

Journal of Biomedical Optics
2015-07-29 | Journal article
DOI: 10.1117/1.jbo.20.7.075010
Part of ISSN: 1083-3668
Source: Self-asserted source
Alex J. Walsh

Collagen density and alignment in responsive and resistant trastuzumab-treated breast cancer xenografts

Journal of Biomedical Optics
2015-02-20 | Journal article
DOI: 10.1117/1.jbo.20.2.026004
Part of ISSN: 1083-3668
Source: Self-asserted source
Alex J. Walsh

Optical metabolic imaging quantifies heterogeneous cell populations

Biomedical Optics Express
2015-02-01 | Journal article
DOI: 10.1364/boe.6.000559
Part of ISSN: 2156-7085
Part of ISSN: 2156-7085
Source: Self-asserted source
Alex J. Walsh

High-throughput measurements of the optical redox ratio using a commercial microplate reader

Journal of Biomedical Optics
2015-01-29 | Journal article
DOI: 10.1117/1.jbo.20.1.010503
Part of ISSN: 1083-3668
Source: Self-asserted source
Alex J. Walsh

Quantitative Optical Imaging of Primary Tumor Organoid Metabolism Predicts Drug Response in Breast Cancer

Cancer Research
2014-09-15 | Journal article
DOI: 10.1158/0008-5472.can-14-0663
Part of ISSN: 0008-5472
Part of ISSN: 1538-7445
Source: Self-asserted source
Alex J. Walsh

In vivo hyperspectral imaging of microvessel response to trastuzumab treatment in breast cancer xenografts

Biomedical Optics Express
2014-07-01 | Journal article
DOI: 10.1364/boe.5.002247
Part of ISSN: 2156-7085
Part of ISSN: 2156-7085
Source: Self-asserted source
Alex J. Walsh

Optical Metabolic Imaging of Treatment Response in Human Head and Neck Squamous Cell Carcinoma

PLoS ONE
2014-03-04 | Journal article
DOI: 10.1371/journal.pone.0090746
Part of ISSN: 1932-6203
Source: Self-asserted source
Alex J. Walsh

The NADH Oxidase ENOX1, a Critical Mediator of Endothelial Cell Radiosensitization, Is Crucial for Vascular Development

Cancer Research
2014-01-01 | Journal article
DOI: 10.1158/0008-5472.can-13-1981
Part of ISSN: 0008-5472
Part of ISSN: 1538-7445
Source: Self-asserted source
Alex J. Walsh

Optical Metabolic Imaging Identifies Glycolytic Levels, Subtypes, and Early-Treatment Response in Breast Cancer

Cancer Research
2013-10-15 | Journal article
DOI: 10.1158/0008-5472.can-13-0527
Part of ISSN: 0008-5472
Part of ISSN: 1538-7445
Source: Self-asserted source
Alex J. Walsh

Quantitative optical imaging of vascular response in vivo in a model of peripheral arterial disease

American Journal of Physiology-Heart and Circulatory Physiology
2013-10-15 | Journal article
DOI: 10.1152/ajpheart.00362.2013
Part of ISSN: 0363-6135
Part of ISSN: 1522-1539
Source: Self-asserted source
Alex J. Walsh
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