David Falck (0000-0003-3908-2376)

Personal information

Biography

David Falck is focusing on method development of targeted glycoproteomic approaches for Fc gamma receptors and immunoglobulins (Ig).
He also contributes to the understanding of proteoform structure-function relationships in the interaction between antibodies and their receptors.
For this, he develops hyphenated affinity separation - mass spectrometry platforms and employs glycoengineering.
Finally, he runs or supports applications in the following fields:
1.) biopharmaceuticals, in collaboration with international industrial partners.
2.) basic glycoimmunology with the following questions
What is the function of antibody glycosylation?
How is the human Fc gamma receptor glycosylated and
how does this influence its interaction with IgG?
3.) clinical studies into the use of glycosylation signatures for personalized medicine.

Activities

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Leiden University Medical Center: Leiden, NL

2018-01 to present | Assistant Professor (Center for Proteomics and Metabolomics (Glycomics group))

Employment

David Falck

https://orcid.org/0000-0003-3908-2376

Personal information

Other IDs

Biography

Activities

Employment (3)

Leiden University Medical Center: Leiden, NL

Leiden University Medical Center: Leiden, NL

Leiden University Medical Center: Leiden, NL

Education and qualifications (2)

Vrije Universiteit Amsterdam: Amsterdam, Noord-Holland, NL

Westfalische Wilhelms-Universität Munster: Munster, Nordrhein-Westfalen, DE

Professional activities (4)

European Glycoscience Community Webinars: Online, GB

Dutch Society for Mass Spectrometry: N.A., NL

Federatie Innovatie Geneesmiddel Onderzoek Nederland (FIGON): WOERDEN, NL

Nederlandse Vereinging voor Massaspectrometrie: N.A., NL

Funding (5)

ReconVac - Elucidating Immune Responses to Glycoconjugate Vaccines

High resolution glycoform analysis of immunoglobulin receptors: A key step towards translational and personalized medicine of antibody based biopharmaceuticals

Exploitation of Glycosylation Signatures for Precision Medicine

Influence of glycoforms on the tryptic digestion efficiency of IgG based biopharmaceuticals

Participation in the Lindau Nobel Laureate Meeting 2013 (Chemistry)

Works (50 of 54)

GlYcoLISA: antigen-specific and subclass-specific IgG Fc glycosylation analysis based on an immunosorbent assay with an LC–MS readout

Benchmarking glycoform-resolved affinity separation – mass spectrometry assays for studying FcγRIIIa binding

Erythropoietin N-glycosylation of Therapeutic Formulations Quantified and Characterized: An Interlab Comparability Study of High-Throughput Methods

Impact of Asialoglycoprotein Receptor and Mannose Receptor Deficiency on Murine Plasma N-glycome Profiles

Specific IgG glycosylation differences precede relapse in PR3-ANCA associated vasculitis patients with and without ANCA rise

Clearance of therapeutic antibody glycoforms after subcutaneous and intravenous injection in a porcine model

Editorial: Immunoglobulin Glycosylation Analysis: State-of-the-Art Methods and Applications in Immunology.

Developments and Perspectives in High-Throughput Protein Glycomics: Enabling the Analysis of Thousands of Samples

A functional spleen contributes to afucosylated IgG in humans

Fc gamma receptor IIIb binding of individual antibody proteoforms resolved by affinity chromatography–mass spectrometry

Serum and Plasma Immunoglobulin G Fc N-Glycosylation Is Stable during Storage

Glycoform-resolved pharmacokinetic studies in a rat model employing glycoengineered variants of a therapeutic monoclonal antibody

A novel glycosidase plate-based assay for the quantification of galactosylation and sialylation on human IgG.

Site-Specific Glycosylation Mapping of Fc Gamma Receptor IIIb from Neutrophils of Individual Healthy Donors

Immunoglobulin G Glycoprofiles are Unaffected by Common Bottom-Up Sample Processing

MS-Based Allotype-Specific Analysis of Polyclonal IgG-Fc <i>N</i>-Glycosylation.

FcγR Binding and ADCC Activity of Human IgG Allotypes.

Site-specific glycosylation mapping of Fc gamma receptor IIIb from neutrophils of health donors

Monitoring of immunoglobulin N- and O-glycosylation in health and disease

Simultaneous Immunoglobulin A and G Glycopeptide Profiling for High-Throughput Applications

A Matrix-Assisted Laser Desorption/Ionization-Mass Spectrometry Assay for the Relative Quantitation of Antennary Fucosylated N-Glycans in Human Plasma.

IgA subclasses have different effector functions associated with distinct glycosylation profiles.

NIST Interlaboratory Study on Glycosylation Analysis of Monoclonal Antibodies: Comparison of Results from Diverse Analytical Methods.

Proteoform-Resolved FcɤRIIIa Binding Assay for Fab Glycosylated Monoclonal Antibodies Achieved by Affinity Chromatography Mass Spectrometry of Fc Moieties.

Glycoform-resolved FcɣRIIIa affinity chromatography–mass spectrometry

Highly sensitive CE-ESI-MS analysis of N-glycans from complex biological samples

ACPA IgG galactosylation associates with disease activity in pregnant patients with rheumatoid arthritis.

Glycosylation of Immunoglobulin G Associates With Clinical Features of Inflammatory Bowel Diseases

High-Throughput Analysis of IgG Fc Glycopeptides by LC-MS.

Affinity purification of erythropoietin from cell culture supernatant combined with MALDI-TOF-MS analysis of erythropoietin N-glycosylation

LaCyTools: A Targeted Liquid Chromatography-Mass Spectrometry Data Processing Package for Relative Quantitation of Glycopeptides.

Dopant Enriched Nitrogen Gas Combined with Sheathless Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry for Improved Sensitivity and Repeatability in Glycopeptide Analysis.

Pregnancy-associated serum N-glycome changes studied by high-throughput MALDI-TOF-MS.

Comparison of Fc N-Glycosylation of Pharmaceutical Products of Intravenous Immunoglobulin G.

Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles-Part 2: Mass spectrometric methods.

MassyTools: A High-Throughput Targeted Data Processing Tool for Relative Quantitation and Quality Control Developed for Glycomic and Glycoproteomic MALDI-MS.

Mass spectrometry for glycosylation analysis of biopharmaceuticals

Glycoforms of Immunoglobulin G Based Biopharmaceuticals Are Differentially Cleaved by Trypsin Due to the Glycoform Influence on Higher-Order Structure.

Metabolic profiling of ligands for the chemokine receptor CXCR3 by liquid chromatography-mass spectrometry coupled to bioaffinity assessment.

Linkage-specific sialic acid derivatization for MALDI-TOF-MS profiling of IgG glycopeptides.

Solution-phase electrochemistry-nuclear magnetic resonance of small organic molecules

High-Resolution Screening: Post-Column Continuous-Flow Bioassays

Introduction to Mass Spectrometry, a Tutorial

Comparison of (bio-)transformation methods for the generation of metabolite-like compound libraries of p38α MAP kinase inhibitors using high-resolution screening.

Combination of biotransformation by P450 BM3 mutants with on-line post-column bioaffinity and mass spectrometric profiling as a novel strategy to diversify and characterize p38α kinase inhibitors

EC-SPE-stripline-NMR analysis of reactive products: a feasibility study.

Tandem mass spectrometry study of p38α kinase inhibitors and related substances.

Development of On-line Liquid Chromatography-Biochemical Detection for Soluble Epoxide Hydrolase Inhibitors in Mixtures.

Development of a profiling strategy for metabolic mixtures by combining chromatography and mass spectrometry with cell-based GPCR signaling.

High-resolution metabolic profiling towards G protein-coupled receptors: rapid and comprehensive screening of histamine H₄ receptor ligands.

Peer review (31 reviews for 17 publications/grants)