Personal information

Emails & domains

Verified email domains

Websites & social links

https://www.maxperutzlabs.ac.at/research/research-groups/otsuka

Activities

Collapse all

Employment (3)

Max Perutz Labs: Vienna, AT

2025-01-01 to present | Associate Professor
Employment
Source: Self-asserted source
Shotaro Otsuka

Max Perutz Labs: Vienna, AT

2019-04-01 to 2024-12-31 | Group Leader
Employment
Source: Self-asserted source
Shotaro Otsuka

EMBL: Heidelberg, DE

2011-04-01 to 2019-03-31
Employment
Source: Self-asserted source
Shotaro Otsuka

Education and qualifications (1)

Kyoto University: Kyoto, JP

2002-04-01 to 2011-03-31
Education
Source: Self-asserted source
Shotaro Otsuka

Works (36)

Integrative spatiotemporal modeling of biomolecular processes: Application to the assembly of the nuclear pore complex

Proceedings of the National Academy of Sciences
2025-03-18 | Journal article
DOI: 10.1073/pnas.2415674122
Contributors: Andrew P. Latham; Wanlu Zhang; Jeremy O. B. Tempkin; Shotaro Otsuka; Jan Ellenberg; Andrej Sali
Source: check_circle
Crossref

Sculpting nuclear envelope identity from the endoplasmic reticulum during the cell cycle

Nucleus
2024-12-31 | Journal article
DOI: 10.1080/19491034.2023.2299632
Contributors: Pallavi Deolal; Julia Scholz; Kaike Ren; Helena Bragulat-Teixidor; Shotaro Otsuka
Source: check_circle
Crossref

Integrative spatiotemporal modeling of biomolecular processes: application to the assembly of the Nuclear Pore Complex

2024-08-08 | Preprint
DOI: 10.1101/2024.08.06.606842
Contributors: Andrew P. Latham; Jeremy O. B. Tempkin; Shotaro Otsuka; Wanlu Zhang; Jan Ellenberg; Andrej Sali
Source: check_circle
Crossref

The endoplasmic reticulum connects to the nucleus by constricted junctions that mature after mitosis

EMBO Reports
2024-06-14 | Journal article
DOI: 10.1038/s44319-024-00175-w
Contributors: Helena Bragulat-Teixidor; Keisuke Ishihara; Gréta Martina Szücs; Shotaro Otsuka
Source: check_circle
Crossref

Correlative light and electron microscopy at defined cell cycle stages in a controlled environment

Correlative Light and Electron Microscopy V
2024-03-26 | Book chapter
DOI: 10.1016/bs.mcb.2024.02.025
Part of ISBN: 9780323951418
Part of ISSN: 0091-679X
Contributors: Helena Bragulat-Teixidor; Shotaro Otsuka
Source: Self-asserted source
Shotaro Otsuka

Structural evidence for elastic tethers connecting separating chromosomes in crane-fly spermatocytes

Life Science Alliance
2023-11 | Journal article
DOI: 10.26508/lsa.202302303
Contributors: Arthur Forer; Shotaro Otsuka
Source: check_circle
Crossref

Structural evidence for elastic tethers connecting separating chromosomes in crane-fly spermatocytes

2023-05-28 | Preprint
DOI: 10.1101/2023.05.28.541924
Contributors: Arthur Forer; Shotaro Otsuka
Source: check_circle
Crossref

The endoplasmic reticulum connects to the nucleus by constricted junctions that mature after open mitosis in mammalian cells

2023-02-01 | Preprint
DOI: 10.1101/2023.01.31.526419
Contributors: Helena Bragulat-Teixidor; Keisuke Ishihara; Gréta Martina Szücs; Shotaro Otsuka
Source: check_circle
Crossref

A quantitative map of nuclear pore assembly reveals two distinct mechanisms

Nature
2023-01-19 | Journal article
DOI: 10.1038/s41586-022-05528-w
Contributors: Shotaro Otsuka; Jeremy O. B. Tempkin; Wanlu Zhang; Antonio Z. Politi; Arina Rybina; M. Julius Hossain; Moritz Kueblbeck; Andrea Callegari; Birgit Koch; Natalia Rosalia Morero et al.
Source: check_circle
Crossref

A mitotic chromatin phase transition prevents perforation by microtubules

Nature
2022-09-01 | Journal article
DOI: 10.1038/s41586-022-05027-y
Contributors: Maximilian W. G. Schneider; Bryan A. Gibson; Shotaro Otsuka; Maximilian F. D. Spicer; Mina Petrovic; Claudia Blaukopf; Christoph C. H. Langer; Paul Batty; Thejaswi Nagaraju; Lynda K. Doolittle et al.
Source: check_circle
Crossref

Visualizing Nuclear Pore Complex Assembly In Situ in Human Cells at Nanometer Resolution by Correlating Live Imaging with Electron Microscopy.

Methods in molecular biology (Clifton, N.J.)
2022-04-13 | Book chapter
DOI: 10.1007/978-1-0716-2337-4_31
PMID: 35412258
Source: Self-asserted source
Shotaro Otsuka

A chromatin phase transition protects mitotic chromosomes against microtubule perforation

2021-07-05 | Other
DOI: 10.1101/2021.07.05.450834
Contributors: Maximilian W. G. Schneider; Bryan A. Gibson; Shotaro Otsuka; Maximilian F.D. Spicer; Mina Petrovic; Claudia Blaukopf; Christoph C. H. Langer; Lynda K. Doolittle; Michael K. Rosen; Daniel W. Gerlich
Source: check_circle
Crossref

A quantitative map of nuclear pore assembly reveals two distinct mechanisms

2021-05-18 | Preprint
DOI: 10.1101/2021.05.17.444137
Contributors: Shotaro Otsuka; Jeremy O. B. Tempkin; Wanlu Zhang; Antonio Z. Politi; Arina Rybina; M. Julius Hossain; Moritz Kueblbeck; Andrea Callegari; Birgit Koch; Natalia Rosalia Morero et al.
Source: check_circle
Crossref

Chemogenetic Control of Nanobodies

Nature Methods
2020-03 | Journal article
DOI: 10.1038/s41592-020-0746-7
Part of ISSN: 1548-7091
Part of ISSN: 1548-7105
Source: Self-asserted source
Shotaro Otsuka

Mechanisms of nuclear pore complex assembly – two different ways of building one molecular machine

FEBS Letters
2018-02 | Journal article
DOI: 10.1002/1873-3468.12905
Contributors: Shotaro Otsuka; Jan Ellenberg
Source: check_circle
Crossref

Postmitotic nuclear pore assembly proceeds by radial dilation of small membrane openings

Nature Structural & Molecular Biology
2018-01-27 | Journal article
DOI: 10.1038/s41594-017-0001-9
Contributors: Shotaro Otsuka; Anna M. Steyer; Martin Schorb; Jean-Karim Hériché; M. Julius Hossain; Suruchi Sethi; Moritz Kueblbeck; Yannick Schwab; Martin Beck; Jan Ellenberg
Source: check_circle
Crossref
grade
Preferred source (of 3)‎

Nuclear pore assembly proceeds by an inside-out extrusion of the nuclear envelope

eLife
2016-09-15 | Journal article
DOI: 10.7554/eLife.19071
Contributors: Shotaro Otsuka; Khanh Huy Bui; Martin Schorb; M Julius Hossain; Antonio Z Politi; Birgit Koch; Mikhail Eltsov; Martin Beck; Jan Ellenberg
Source: check_circle
Crossref

Dissecting in vivo steady-state dynamics of karyopherin-dependent nuclear transport

Mol Biol Cell
2016-01-01 | Journal article
DOI: 10.1091/mbc.E15-08-0601
Source: Self-asserted source
Shotaro Otsuka

Imaging the assembly, structure, and function of the nuclear pore inside cells.

2014 | Journal article
DOI:

10.1016/B978-0-12-417160-2.00010-2

PMID:

24857732
Contributors: Otsuka, Shotaro; Szymborska, Anna; Ellenberg, Jan
Source: check_circle
EMBL-EBI

Intermolecular disulfide bonds between nucleoporins regulate karyopherin-dependent nuclear transport.

2013-07 | Journal article
DOI:

10.1242/jcs.124172

PMID:

23641069
Contributors: Yoshimura, Shige H; Otsuka, Shotaro; Kumeta, Masahiro; Taga, Mariko; Takeyasu, Kunio
Source: check_circle
EMBL-EBI

Nup358, a nucleoporin, functions as a key determinant of the nuclear pore complex structure remodeling during skeletal myogenesis.

2011-02 | Journal article
DOI:

10.1111/j.1742-4658.2010.07982.x

PMID:

21205196
Contributors: Asally, Munehiro; Yasuda, Yoshinari; Oka, Masahiro; Otsuka, Shotaro; Yoshimura, Shige H; Takeyasu, Kunio; Yoneda, Yoshihiro
Source: check_circle
EMBL-EBI

Individual binding pockets of importin-beta for FG-nucleoporins have different binding properties and different sensitivities to RanGTP.

2008-10 | Journal article
DOI:

10.1073/pnas.0802647105

PMID:

18845677

PMC:

PMC2570979
Contributors: Otsuka, Shotaro; Iwasaka, Shizuka; Yoneda, Yoshihiro; Takeyasu, Kunio; Yoshimura, Shige H
Source: check_circle
EMBL-EBI

Nuclear architecture and chromatin dynamics revealed by atomic force microscopy in combination with biochemistry and cell biology.

2008-04 | Journal article
DOI:

10.1007/s00424-007-0431-z

PMID:

18172599
Contributors: Hirano, Yasuhiro; Takahashi, Hirohide; Kumeta, Masahiro; Hizume, Kohji; Hirai, Yuya; Otsuka, Shotaro; Yoshimura, Shige H; Takeyasu, Kunio
Source: check_circle
EMBL-EBI

Development of glutathione-coupled cantilever for the single-molecule force measurement by scanning force microscopy.

2006-07 | Journal article
DOI:

10.1016/j.febslet.2006.06.032

PMID:

16806198
Contributors: Yoshimura, Shige H; Takahashi, Hirohide; Otsuka, Shotaro; Takeyasu, Kunio
Source: check_circle
EMBL-EBI

FIB-SEM of a dividing cell at 11.2 min after anaphase onset

Dataset
EMPIAR: EMPIAR-10109
Source: check_circle
EMBL-EBI

FIB-SEM of a dividing cell at 3.1 min after anaphase onset

Dataset
EMPIAR: EMPIAR-10100
Source: check_circle
EMBL-EBI

FIB-SEM of a dividing cell at 3.9 min after anaphase onset

Dataset
EMPIAR: EMPIAR-10102
Source: check_circle
EMBL-EBI

FIB-SEM of a dividing cell at 4.3 min after anaphase onset

Dataset
EMPIAR: EMPIAR-10103
Source: check_circle
EMBL-EBI

FIB-SEM of a dividing cell at 5.3 min after anaphase onset

Dataset
EMPIAR: EMPIAR-10104
Source: check_circle
EMBL-EBI

FIB-SEM of a dividing cell at 5.7 min after anaphase onset

Dataset
EMPIAR: EMPIAR-10105
Source: check_circle
EMBL-EBI

FIB-SEM of a dividing cell at 6.3 min after anaphase onset

Dataset
EMPIAR: EMPIAR-10101
Source: check_circle
EMBL-EBI

Raw 2d tomographic tilt series of a dividing cell

Dataset
EMPIAR: EMPIAR-10116
Source: check_circle
EMBL-EBI

Tomograms and the raw 2d tilt series of ER-ER junctions of HeLa cells in interphase

Dataset
EMPIAR: EMPIAR-12050
Source: check_circle
EMBL-EBI

Tomograms and the raw 2d tilt series of ER-ER/nuclear envelope junctions of HeLa cells in late anaphase

Dataset
EMPIAR: EMPIAR-12051
Source: check_circle
EMBL-EBI

Tomograms and the raw 2d tilt series of ER-nuclear envelope junctions of HeLa cells in early telophase

Dataset
EMPIAR: EMPIAR-12048
Source: check_circle
EMBL-EBI

Tomograms and the raw 2d tilt series of ER-nuclear envelope junctions of HeLa cells in interphase

Dataset
EMPIAR: EMPIAR-12025
Source: check_circle
EMBL-EBI

Peer review (5 reviews for 4 publications/grants)

Review activity for Cellular and molecular life sciences (1)
Review activity for EMBO journal. (1)
Review activity for Journal of Molecular Biology. (2)
Review activity for Life science alliance. (1)
How many people are using ORCID?