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Scopus Author ID: 57212330806

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Employment (2)

Wageningen University and Research Wageningen Bioveterinary Research: Lelystad, Flevoland, NL

1995-05-01 to present
Employment
Source: Self-asserted source
Michiel Harmsen

Vrije Universiteit Amsterdam: Amsterdam, Noord-Holland, NL

1990-02-01 to 1995-02-01 | PhD student (Biochemistry)
Employment
Source: Self-asserted source
Michiel Harmsen

Education and qualifications (1)

Radboud University Nijmegen: Nijmegen, Gelderland, NL

1982 to 1988 | Master Biology
Education
Source: Self-asserted source
Michiel Harmsen

Works (45)

Foot-and-mouth disease virus antigenic landscape and reduced immunogenicity elucidated in atomic detail

Nature Communications
2024-10-10 | Journal article
DOI: 10.1038/s41467-024-53027-5
Contributors: Haozhou Li; Pan Liu; Hu Dong; Aldo Dekker; Michiel M. Harmsen; Huichen Guo; Xiangxi Wang; Shiqi Sun
Source: check_circle
Crossref

Mapping of foot-and-mouth disease virus antigenic sites recognized by single-domain antibodies reveals different 146S particle specific sites and particle flexibility

Frontiers in Veterinary Science
2023 | Journal article
DOI: 10.3389/fvets.2022.1040802
EID:

2-s2.0-85146843226

Part of ISSN: 22971769
Contributors: Harmsen, M.M.; Li, H.; Sun, S.; van der Poel, W.H.M.; Dekker, A.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Single-Domain Antibody Multimers for Detection of Botulinum Neurotoxin Serotypes C, D, and Their Mosaics in Endopep-MS

Toxins
2023-09-17 | Journal article
DOI: 10.3390/toxins15090573
Contributors: Michiel M. Harmsen; Jan C. Cornelissen; Fimme J. van der Wal; Jan H. W. Bergervoet; Miriam Koene
Source: check_circle
Crossref
grade
Preferred source (of 2)‎

Small-Scale Secretory VHH Expression in Saccharomyces cerevisiae

Methods in Molecular Biology
2022 | Book chapter
DOI: 10.1007/978-1-0716-2075-5_8
EID:

2-s2.0-85124582988

Part of ISSN: 19406029 10643745
Contributors: Harmsen, M.M.; van Hagen-van Setten, M.; Willemsen, P.T.J.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Structural and molecular basis for foot-and-mouth disease virus neutralization by two potent protective antibodies

Protein and Cell
2022 | Journal article
DOI: 10.1007/s13238-021-00828-9
EID:

2-s2.0-85101211252

Part of ISSN: 16748018 1674800X
Contributors: Dong, H.; Liu, P.; Bai, M.; Wang, K.; Feng, R.; Zhu, D.; Sun, Y.; Mu, S.; Li, H.; Harmsen, M. et al.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

A novel single-domain antibody multimer that potently neutralizes tetanus neurotoxin

Vaccine: X
2021 | Journal article
DOI: 10.1016/j.jvacx.2021.100099
EID:

2-s2.0-85108690602

Part of ISSN: 25901362
Contributors: de Smit, H.; Ackerschott, B.; Tierney, R.; Stickings, P.; Harmsen, M.M.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Structural and molecular basis for foot-and-mouth disease virus neutralization by two potent protective antibodies

bioRxiv
2021 | Other
DOI: 10.1101/2020.12.31.424923
EID:

2-s2.0-85100772264

Part of ISSN: 26928205
Contributors: Dong, H.; Liu, P.; Bai, M.; Wang, K.; Feng, R.; Zhu, D.; Sun, Y.; Mu, S.; Li, H.; Harmsen, M. et al.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Novel Capsid-Specific Single-Domain Antibodies with Broad Foot-and-Mouth Disease Strain Recognition Reveal Differences in Antigenicity of Virions, Empty Capsids, and Virus-Like Particles

Vaccines
2021-06-08 | Journal article
DOI: 10.3390/vaccines9060620
Contributors: Haozhou Li; Aldo Dekker; Shiqi Sun; Alison Burman; Jeroen Kortekaas; Michiel M. Harmsen
Source: check_circle
Crossref
grade
Preferred source (of 4)‎

Multimeric single-domain antibody complexes protect against bunyavirus infections

eLife
2020 | Journal article
DOI: 10.7554/eLife.52716
EID:

2-s2.0-85083812029

Part of ISSN: 2050084X
Contributors: Schreur, P.J.W.; van de Water, S.; Harmsen, M.; Bermúdez-Méndez, E.; Drabek, D.; Grosveld, F.; Wernike, K.; Beer, M.; Aebischer, A.; Daramola, O. et al.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Chimeric O1K foot-and-mouth disease virus with SAT2 outer capsid as an FMD vaccine candidate

Scientific Reports
2018 | Journal article
DOI: 10.1038/s41598-018-31856-x
EID:

2-s2.0-85053213670

Part of ISSN: 20452322
Contributors: Kotecha, A.; Perez-Martin, E.; Harvey, Y.; Zhang, F.; Ilca, S.L.; Fry, E.E.; Jackson, B.; Maree, F.; Scott, K.; Hecksel, C.W. et al.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Isolation of single-domain antibody fragments that preferentially detect intact (146s) particles of foot-and-mouth disease virus for use in vaccine quality control

Frontiers in Immunology
2017 | Journal article
DOI: 10.3389/fimmu.2017.00960
EID:

2-s2.0-85027865981

Part of ISSN: 16643224
Contributors: Harmsen, M.M.; Seago, J.; Perez, E.; Charleston, B.; Eblé, P.L.; Dekker, A.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Stabilizing effects of excipients on dissociation of intact (146S) foot-and-mouth disease virions into 12S particles during storage as oil-emulsion vaccine

Vaccine
2015 | Journal article
DOI: 10.1016/j.vaccine.2015.03.066
EID:

2-s2.0-84928581683

Part of ISSN: 18732518 0264410X
Contributors: Harmsen, M.M.; Fijten, H.P.D.; Westra, D.F.; Dekker, A.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Identification of the structural basis of thermal lability of a virus provides a rationale for improved vaccines

Structure
2014 | Journal article
DOI: 10.1016/j.str.2014.08.019
EID:

2-s2.0-84908504102

Part of ISSN: 18784186 09692126
Contributors: Rincón, V.; Rodríguez-Huete, A.; López-Argüello, S.; Ibarra-Molero, B.; Sanchez-Ruiz, J.M.; Harmsen, M.M.; Mateu, M.G.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Isolation of panels of llama single-domain antibody fragments binding all nine neuraminidase subtypes of influenza a virus

Antibodies
2013 | Journal article
DOI: 10.3390/antib2020168
EID:

2-s2.0-84903878121

Part of ISSN: 20734468
Contributors: Harmsen, M.M.; Blokker, J.C.; Pritz-Verschuren, S.B.; Bartelink, W.; van der Burg, H.; Koch, G.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier
grade
Preferred source (of 2)‎

The effect of uniform capture molecule orientation on biosensor sensitivity: Dependence on analyte properties

Biosensors and Bioelectronics
2013 | Journal article
DOI: 10.1016/j.bios.2012.07.027
EID:

2-s2.0-84868633422

Part of ISSN: 09565663 18734235
Contributors: Trilling, A.K.; Harmsen, M.M.; Ruigrok, V.J.B.; Zuilhof, H.; Beekwilder, J.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Characterization of epitope-tagged foot-and-mouth disease virus

Journal of General Virology
2012 | Journal article
DOI: 10.1099/vir.0.043521-0
EID:

2-s2.0-84867495888

Part of ISSN: 00221317 14652099
Contributors: Seago, J.; Jackson, T.; Doel, C.; Fry, E.; Stuart, D.; Harmsen, M.M.; Charleston, B.; Juleff, N.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Effect of natural and chimeric haemagglutinin genes on influenza A virus replication in baby hamster kidney cells

Journal of Biotechnology
2012 | Journal article
DOI: 10.1016/j.jbiotec.2012.10.005
EID:

2-s2.0-84868468727

Part of ISSN: 18734863 01681656
Contributors: van Wielink, R.; Harmsen, M.M.; Martens, D.E.; de Leeuw, O.S.; Peeters, B.P.H.; Wijffels, R.H.; Moormann, R.J.M.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Improved functional immobilization of llama single-domain antibody fragments to polystyrene surfaces using small peptides

Journal of Immunoassay and Immunochemistry
2012 | Journal article
DOI: 10.1080/15321819.2011.634473
EID:

2-s2.0-84863438450

Part of ISSN: 15321819 15324230
Contributors: Harmsen, M.M.; Fijten, H.P.D.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Mutations in the M-gene segment can substantially increase replication efficiency of NS1 deletion influenza a virus in MDCK cells

Journal of Virology
2012 | Journal article
DOI: 10.1128/JVI.01725-12
EID:

2-s2.0-84869118918

Part of ISSN: 0022538X 10985514
Contributors: van Wielink, R.; Harmsen, M.M.; Martens, D.E.; Peeters, B.P.H.; Wijffels, R.H.; Moormanna, R.J.M.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Adaptation of a Madin-Darby canine kidney cell line to suspension growth in serum-free media and comparison of its ability to produce avian influenza virus to Vero and BHK21 cell lines

Journal of Virological Methods
2011 | Journal article
DOI: 10.1016/j.jviromet.2010.09.029
EID:

2-s2.0-78650569516

Part of ISSN: 01660934
Contributors: van Wielink, R.; Kant-Eenbergen, H.C.M.; Harmsen, M.M.; Martens, D.E.; Wijffels, R.H.; Coco-Martin, J.M.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Effect of thiomersal on dissociation of intact (146S) foot-and-mouth disease virions into 12S particles as assessed by novel ELISAs specific for either 146S or 12S particles

Vaccine
2011 | Journal article
DOI: 10.1016/j.vaccine.2011.01.069
EID:

2-s2.0-79952251317

Part of ISSN: 0264410X
Contributors: Harmsen, M.M.; Fijten, H.P.D.; Westra, D.F.; Coco-Martin, J.M.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

MDCK cell line with inducible allele B NS1 expression propagates delNS1 influenza virus to high titres

Vaccine
2011 | Journal article
DOI: 10.1016/j.vaccine.2011.07.037
EID:

2-s2.0-80052335815

Part of ISSN: 0264410X 18732518
Contributors: Van Wielink, R.; Harmsen, M.M.; Martens, D.E.; Peeters, B.P.H.; Wijffels, R.H.; Moormann, R.J.M.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Parenteral vaccination of mammalian livestock with Newcastle disease virus-based vector vaccines offers optimal efficacy and safety

Bioengineered Bugs
2011 | Journal article
DOI: 10.4161/bbug.2.1.13349
EID:

2-s2.0-78751620653

Part of ISSN: 19491026 19491018
Contributors: Harmsen, M.M.; Antonis, A.F.G.; Moormann, R.J.M.; Kortekaas, J.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Characterization of foot-and-mouth disease virus antigen by surface-enhanced laser desorption ionization-time of flight-mass spectrometry in aqueous and oil-emulsion formulations

Vaccine
2010 | Journal article
DOI: 10.1016/j.vaccine.2010.02.084
EID:

2-s2.0-77950517299

Part of ISSN: 0264410X
Contributors: Harmsen, M.M.; Jansen, J.; Westra, D.F.; Coco-Martin, J.M.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Enhancement of toxin- and virus-neutralizing capacity of single-domain antibody fragments by N-glycosylation

Applied Microbiology and Biotechnology
2009 | Journal article
DOI: 10.1007/s00253-009-2029-1
EID:

2-s2.0-70349680965

Part of ISSN: 01757598
Contributors: Harmsen, M.M.; Van Solt, C.B.; Fijten, H.P.D.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Passive immunization with llama single-domain antibody fragments reduces foot-and-mouth disease transmission between pigs

Vaccine
2009 | Journal article
DOI: 10.1016/j.vaccine.2009.01.110
EID:

2-s2.0-61349157275

Part of ISSN: 0264410X
Contributors: Harmsen, M.M.; Fijten, H.P.D.; Engel, B.; Dekker, A.; Eblé, P.L.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Passive immunization of pigs with bispecific llama single-domain antibody fragments against foot-and-mouth disease and porcine immunoglobulin

Veterinary Microbiology
2008 | Journal article
DOI: 10.1016/j.vetmic.2008.04.030
EID:

2-s2.0-51649091611

Part of ISSN: 03781135
Contributors: Harmsen, M.M.; Fijten, H.P.D.; Dekker, A.; Eblé, P.L.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Passive immunization of guinea pigs with llama single-domain antibody fragments against foot-and-mouth disease

Veterinary Microbiology
2007 | Journal article
DOI: 10.1016/j.vetmic.2006.10.029
EID:

2-s2.0-33846820458

Part of ISSN: 03781135
Contributors: Harmsen, M.M.; van Solt, C.B.; Fijten, H.P.D.; van Keulen, L.; Rosalia, R.A.; Weerdmeester, K.; Cornelissen, A.H.M.; De Bruin, M.G.M.; Eblé, P.L.; Dekker, A.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Properties, production, and applications of camelid single-domain antibody fragments

Applied Microbiology and Biotechnology
2007 | Journal article
DOI: 10.1007/s00253-007-1142-2
EID:

2-s2.0-35348819390

Part of ISSN: 01757598
Contributors: Harmsen, M.M.; De Haard, H.J.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

A case of severe sarcoptic mange in alpacas (Lama pacos),Een geval van ernstige Sarcoptes-schurft bij alpaca's (Lama pacos)

Tijdschrift voor Diergeneeskunde
2006 | Journal article
EID:

2-s2.0-33646371007

Part of ISSN: 00407453
Contributors: Borgsteede, F.H.M.; Timmerman, A.; Harmsen, M.M.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Neutralisation of venom-induced haemorrhage by IgG from camels and llamas immunised with viper venom and also by endogenous, non-IgG components in camelid sera

Toxicon
2006 | Journal article
DOI: 10.1016/j.toxicon.2005.10.017
EID:

2-s2.0-33644512108

Part of ISSN: 00410101
Contributors: Harrison, R.A.; Hasson, S.S.; Harmsen, M.; Laing, G.D.; Conrath, K.; Theakston, R.D.G.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Selection and optimization of proteolytically stable llama single-domain antibody fragments for oral immunotherapy

Applied Microbiology and Biotechnology
2006 | Journal article
DOI: 10.1007/s00253-005-0300-7
EID:

2-s2.0-33747738604

Part of ISSN: 01757598
Contributors: Harmsen, M.M.; Van Solt, C.B.; Van Zijderveld-Van Bemmel, A.M.; Niewold, T.A.; Van Zijderveld, F.G.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Escherichia coli F4 fimbriae specific llama single-domain antibody fragments effectively inhibit bacterial adhesion in vitro but poorly protect against diarrhoea

Veterinary Microbiology
2005 | Journal article
DOI: 10.1016/j.vetmic.2005.09.005
EID:

2-s2.0-27644445089

Part of ISSN: 03781135
Contributors: Harmsen, M.M.; Van Solt, C.B.; Hoogendoorn, A.; Van Zijderveld, F.G.; Niewold, T.A.; Van Der Meulen, J.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Fasciola hepatica procathepsin L3 protein expressed by a baculovirus recombinant can partly protect rats against fasciolosis

Vaccine
2005 | Journal article
DOI: 10.1016/j.vaccine.2004.12.007
EID:

2-s2.0-16244390514

Part of ISSN: 0264410X
Contributors: Reszka, N.; Cornelissen, J.B.W.J.; Harmsen, M.M.; Bieńkowska-Szewczyk, K.; De Bree, J.; Boersma, W.J.; Rijsewijk, F.A.M.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Prolonged in vivo residence times of llama single-domain antibody fragments in pigs by binding to porcine immunoglobulins

Vaccine
2005 | Journal article
DOI: 10.1016/j.vaccine.2005.05.017
EID:

2-s2.0-24144458917

Part of ISSN: 0264410X
Contributors: Harmsen, M.M.; Van Solt, C.B.; Fijten, H.P.D.; Van Setten, M.C.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Identification of a novel Fasciola hepatica cathepsin L protease containing protective epitopes within the propeptide

International Journal for Parasitology
2004 | Journal article
DOI: 10.1016/j.ijpara.2003.12.011
EID:

2-s2.0-1942477617

Part of ISSN: 00207519
Contributors: Harmsen, M.M.; Cornelissen, J.B.W.J.; Buijs, H.E.C.M.; Boersma, W.J.A.; Jeurissen, S.H.M.; Van Milligen, F.J.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Stimulation of chymosin secretion by simultaneous expression with chymosin-binding llama single-domain antibody fragments in yeast

Applied Microbiology and Biotechnology
2002 | Journal article
DOI: 10.1007/s00253-002-1136-z
EID:

2-s2.0-0037213327

Part of ISSN: 01757598
Contributors: Harmsen, M.M.; Smits, C.B.; De Geus, B.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Early immunodiagnosis of fasciolosis in ruminants using recombinant Fasciola hepatica cathepsin L-like protease

International Journal for Parasitology
2001 | Journal article
DOI: 10.1016/S0020-7519(01)00175-8
EID:

2-s2.0-0035002767

Part of ISSN: 00207519
Contributors: Cornelissen, J.B.W.J.; Gaasenbeek, C.P.H; Borgsteede, F.H.M; Holland, W.G.; Harmsen, M.M.; Boersma, W.J.A
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Llama heavy-chain V regions consist of at least four distinct subfamilies revealing novel sequence features

Molecular Immunology
2000 | Journal article
DOI: 10.1016/S0161-5890(00)00081-X
EID:

2-s2.0-0034524838

Part of ISSN: 01615890
Contributors: Harmsen, M.M.; Ruuls, R.C.; Nijman, I.J.; Niewold, T.A.; Frenken, L.G.J.; De Geus, B.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Comparison of physical chemical properties of llama V(HH) antibody fragments and mouse monoclonal antibodies

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology
1999 | Journal article
DOI: 10.1016/S0167-4838(99)00030-8
EID:

2-s2.0-0032955291

Part of ISSN: 01674838
Contributors: Van Der Linden, R.H.J.; Frenken, L.G.J.; De Geus, B.; Harmsen, M.M.; Ruuls, R.C.; Stok, W.; De Ron, L.; Wilson, S.; Davis, P.; Verrips, C.T.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Expression of the house dust mite allergen Der p 2 in the baker's yeast Saccharomyces cerevisiae

Clinical and Experimental Allergy
1998 | Journal article
DOI: 10.1046/j.1365-2222.1998.00164.x
EID:

2-s2.0-0031962992

Part of ISSN: 09547894
Contributors: Hakkaart, G.A.J.; Harmsen, M.M.; Chua, K.-Y.; Thomas, W.R.; Aalberse, R.C.; Van Ree, R.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Prevention of diarrhoea using pathogen specific monoclonal antibodies in an experimental enterotoxigenic e. coli infection in germfree piglets

Veterinary Quarterly
1998 | Journal article
DOI: 10.1080/01652176.1998.9694978
EID:

2-s2.0-0031867240

Part of ISSN: 18755941 01652176
Contributors: de Geus, B.; Harmsen, M.; van Zijderveld, F.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Overexpression of binding protein and disruption of the PMR1 gene synergistically stimulate secretion of bovine prochymosin but not plant Thaumatin in yeast

Applied Microbiology and Biotechnology
1996 | Journal article
DOI: 10.1007/BF00166231
EID:

2-s2.0-0029826047

Part of ISSN: 01757598
Contributors: Harmsen, M.M.; Bruyne, M.I.; Raué, H.A.; Maat, J.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Effect of a pmr1 disruption and different signal sequences on the intracellular processing and secretion of Cyamopsis tetragonoloba α-galactosidase by Saccharomyces cerevisiae

Gene
1993 | Journal article
DOI: 10.1016/0378-1119(93)90318-W
EID:

2-s2.0-0027339981

Part of ISSN: 03781119
Contributors: Harmsen, M.M.; Langedijk, A.C.; van Tuinen, E.; Geerse, R.H.; Raué, H.A.; Maat, J.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

The nifH promoter region of Rhizobium leguminosarum: nucleotide sequence and promoter elements controlling activation by NifA protein

Gene
1990 | Journal article
DOI: 10.1016/0378-1119(90)90492-A
EID:

2-s2.0-0025371880

Part of ISSN: 03781119
Contributors: Roelvink, P.W.; Harmsen, M.; van Kammen, A.; van den Bos, R.C.
Source: Self-asserted source
Michiel Harmsen via Scopus - Elsevier

Peer review (7 reviews for 5 publications/grants)

Review activity for 3 biotech. (1)
Review activity for Applied microbiology and biotechnology (1)
Review activity for International journal of biological macromolecules. (2)
Review activity for Vaccine. (2)
Review activity for Virology. (1)
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