Personal information

Sperm Physiology, Semen cryopreservation, Reproduction

Activities

Employment (1)

Universidad Austral de Chile: Valdivia, Región de Los Rios, CL

2008-03-01 to present | Profesor Asociado (Instituto de Ciencia Animal, Facultad de Ciencias Veterinarias)
Employment
Source: Self-asserted source
Alfredo Ramírez

Education and qualifications (3)

Universidad Austral de Chile: Valdivia, Región de Los Rios, CL

2007 | Doctor en Ciencias, Mención Biología Celular y Molecular
Education
Source: Self-asserted source
Alfredo Ramírez

Universidad Austral de Chile: Valdivia, Región de Los Rios, CL

2002 | Magister en Ciencias, Mención Genética (Facultad de Ciencias)
Education
Source: Self-asserted source
Alfredo Ramírez

Pontificia Universidad Católica de Chile: Santiago, Región Metropolitana, CL

1993 | Profesor de Biología y Ciencias Naturales
Education
Source: Self-asserted source
Alfredo Ramírez

Professional activities (1)

Sociedad Chilena de Reproduccion y Desarrollo: Valdivia, Región de Los Ríos, CL

2010-09 to present
Membership
Source: Self-asserted source
Alfredo Ramírez

Funding (7)

Desarrollo de un plan mular y núcleo genético asnal catalán en Chile, apto para uso en operaciones militares y en el Plan Nacional de Fomento Equino

2023-06 to 2025-06 | Award
Agencia Nacional de Investigación y Desarrollo (Santiago, CL)
Source: Self-asserted source
Alfredo Ramírez

La función mitocondrial en el espermatozoide, más allá de la energética

2021-12 to 2022-12 | Award
Agencia Nacional de Investigación y Desarrollo (Santiago, Región de Los Rios, CL)
Source: Self-asserted source
Alfredo Ramírez

Fortalecimiento de la investigación en biología espermática y su desarrollo biotecnológico, con participación en docencia de pregrado y activa vinculación con el Programa de Doctorado de Ciencias Veterinarias

2016-01 to 2017-12 | Award
Comisión Nacional de Investigación Científica y Tecnológica (Valdivia, Región de Los Rios, CL)
Source: Self-asserted source
Alfredo Ramírez

“Desarrollo y Validación de un nuevo Kit de Análisis de Capacitación Espermática, Basado en la Detección de Fosfoproteínas

2013-03 to 2014-03 | Award
Comisión Nacional de Investigación Científica y Tecnológica (Valdivia, Región de Los Rios, CL)
Source: Self-asserted source
Alfredo Ramírez

Generación de un banco de semen de caballos fina sangre de tiro pesado, a partir del diseño de protocolos de criopreservación para uso en inseminación artificial (IA) en el plan nacional de fomento equino (PNFE)

2010-03 to 2013-08 | Award
Fondo de Fomento al Desarrollo Científico y Tecnológico (Valdivia, Región de Los Rios, CL)
Source: Self-asserted source
Alfredo Ramírez

Evaluación de la enzima AMPK como diana farmacológica para el control de la criocapacitación de espermatozoides bovinos

2009-03 to 2011-03 | Award
Dirección de Investigación, Universidad Austral de Chile (Valdivia, Región de Los Rios, CL)
Source: Self-asserted source
Alfredo Ramírez

Presencia y funcionalidad de los receptores canabinoides (CB1) y de dopamina (D2R) en espermatozoides de mamíferos.

2004-06 to 2006-06 | Award
Dirección de Investigación, Universidad Austral de Chile (Valdivia, Región de Los Rios, CL)
Source: Self-asserted source
Alfredo Ramírez

Works (37)

An assessment of the Chilean COVID-19 surveillance program through the comparison between reported and true SARS-CoV-2 infection prevalence: A case study of three urban centers in southern Chile

Public Health
2025-02 | Journal article
Contributors: Cristóbal Verdugo; Claudio Verdugo; Alberto Fica; Felipe Hernández; Alfredo Ramírez-Reveco; Anita Plaza; Natalia Castro; Maximiliano Hernández-Riquelme; Gerardo Acosta-Jamett
Source: check_circle
Crossref

Performance estimation of two in-house ELISA assays for COVID-19 surveillance through the combined detection of anti-SARS-CoV-2 IgA, IgM, and IgG immunoglobulin isotypes

PLOS ONE
2023-02-06 | Journal article
Contributors: Alfredo Ramírez-Reveco; Maemu Petronella Gededzha; Gerardo Velásquez; Christopher Aros; Gabriela Navarrete; Franz Villarroel-Espíndola; Maritza Navarrete; Alberto Fica; Anita Plaza; Natalia Castro et al.
Source: check_circle
Crossref

Performance estimation of two in-house ELISA assays for COVID-19 surveillance through the combined detection of anti-SARS-CoV-2 IgA, IgM, and IgG immunoglobulin isotypes

2022-06-14 | Preprint
Contributors: Alfredo Ramírez-Reveco; Gerardo Velázquez; Christopher Aros; Gabriela Navarrete; Franz Villarroel-Espindola; Maritza Navarrete; Alberto Fica; Anita Plaza; Natalia Castro; Claudio Verdugo et al.
Source: check_circle
Crossref

Semen Quality and Freezability Analyses in the Ejaculates of Two Poitou Donkeys in the Southern Hemisphere

Frontiers in Veterinary Science
2021-09-03 | Journal article
Part of ISSN: 2297-1769
Source: Self-asserted source
Alfredo Ramírez

Analytical evaluation of an immunomagnetic separation PCR assay to detect pathogenic <i>Leptospira</i> in cattle urine samples obtained under field conditions

Journal of Veterinary Diagnostic Investigation
2021-01 | Journal article
Part of ISSN: 1040-6387
Part of ISSN: 1943-4936
Source: Self-asserted source
Alfredo Ramírez

Temperature, but not excess of glycogen, regulates “in vitro” AMPK activity in muscle samples of steer carcasses

PLOS ONE
2021-01-28 | Journal article
Contributors: Pablo Strobel; Marcio de Souza Duarte; Alex Galaz; Franz Villaroel-Espíndola; Ariel Apaoblaza; Juan Carlos Slebe; Nancy Jerez-Timaure; Carmen Gallo; Alfredo Ramírez-Reveco
Source: check_circle
Crossref

Chronic Inflammatory Lameness Increases Cytokine Concentration in the Spinal Cord of Dairy Cows

Frontiers in Veterinary Science
2020 | Journal article
EID:

2-s2.0-85082547349

Part of ISBN:

22971769

Contributors: Herzberg, D.; Strobel, P.; Ramirez-Reveco, A.; Werner, M.; Bustamante, H.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Semen quality and freezability analysis during breeding and non‐breeding seasons in heavy draft stallions in southern Chile

Andrologia
2020-12 | Journal article
Contributors: Francisca Ebel; Andrés Vallejos; Gonzalo Gajardo; Omar Ulloa; Eduardo Clavel; Joan Enric Rodríguez‐Gil; Alfredo Ramírez‐Reveco
Source: check_circle
Crossref

Effectiveness of copper ions against Mycobacterium avium subsp. paratuberculosis and bacterial communities in naturally contaminated raw cow’s milk

Journal of Applied Microbiology
2020-11-05 | Journal article
Part of ISSN: 1364-5072
Part of ISSN: 1365-2672
Source: Self-asserted source
Alfredo Ramírez
grade
Preferred source (of 2)‎

A comparative evaluation of dye-based and probe-based RT-qPCR assay for the screening of SARS-CoV-2 using individual and pooled-sample testing.

[]
2020-06-03 | Preprint
Source: Self-asserted source
Alfredo Ramírez

Temperature, but not excess of glycogen, regulate post-mortem AMPK activity in muscle of steer carcasses.

2020-02-10 | Preprint
Contributors: Pablo Strobel; A Galaz; Franz Villaroel-Espíndola; Ariel Apaoblaza; Juan Carlos Slebe; Nancy Jerez-Timaure; Carmen Gallo; Alfredo Ramírez-Reveco
Source: check_circle
Crossref

LED-based red light photostimulation improves short-term response of cooled boar semen exposed to thermal stress at 37°C

Andrologia
2019 | Journal article
EID:

2-s2.0-85060259226

Part of ISBN:

14390272 03034569

Contributors: Pezo, F.; Zambrano, F.; Uribe, P.; Ramírez-Reveco, A.; Romero, F.; Sanchéz, R.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Spinal reactive oxygen species and oxidative damage mediate chronic pain in lame dairy cows

Animals
2019 | Journal article
EID:

2-s2.0-85073478476

Part of ISBN:

20762615

Contributors: Herzberg, D.; Strobel, P.; Chihuailaf, R.; Ramirez-Reveco, A.; Müller, H.; Werner, M.; Bustamante, H.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

“In vitro” capacitation and further progesterone-induced acrosome exocytosis are linked to specific changes in the expression and location of threonine phosphorylation of boar spermatozoa

Reproduction in Domestic Animals
2019 | Journal article
EID:

2-s2.0-85068079326

Part of ISBN:

14390531 09366768

Contributors: Ramió-Lluch, L.; Prieto, O.B.; Ramírez, A.; Fernández-Novell, J.M.; Peña, A.; Rodríguez-Gil, J.E.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Early differential gene expression in beef Longissimus thoracis muscles from carcasses with normal (<5.8) and high (>5.9) ultimate pH

Meat Science
2019-07 | Journal article
Contributors: Nancy Jerez-Timaure; Carmen Gallo; Alfredo Ramírez-Reveco; Gonzalo Greif; Pablo Strobel; Antonia V.F. Pedro; Francisco J. Morera
Source: check_circle
Crossref
grade
Preferred source (of 2)‎

In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions

BMC Microbiology
2018 | Journal article
EID:

2-s2.0-85055794363

Part of ISBN:

14712180

Contributors: Steuer, P.; Avilez, C.; Tejeda, C.; Gonzalez, N.; Ramirez-Reveco, A.; Ulloa, F.; Mella, A.; Grant, I.R.; Collins, M.T.; Salgado, M.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Effect of season, supplementation and fasting on glycolytic potential and activity of AMP-activated protein kinase, glycogen phosphorylase and glycogen debranching enzyme in grass-fed steers as determined in Longissimus lumborum muscle

Livestock Science
2017 | Journal article
EID:

2-s2.0-85020196804

Part of ISBN:

18711413

Contributors: Apaoblaza, A.; Strobel, P.; Ramírez-Reveco, A.; Jeréz-Timaure, N.; Monti, G.; Gallo, C.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Effects of storage time on the motility, mortality and calcium levels of Atlantic salmon Salmo salar spermatozoa

Journal of Fish Biology
2017 | Journal article
EID:

2-s2.0-85010699908

Part of ISBN:

10958649 00221112

Contributors: Parodi, J.; Guerra, G.; Cuevas, M.; Ramírez-Reveco, A.; Romero, F.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Neuronal signaling repertoire in the mammalian sperm functionality

Biology of Reproduction
2017 | Journal article
EID:

2-s2.0-85022331314

Part of ISBN:

15297268 00063363

Contributors: Ramírez-Reveco, A.; Villarroel-Espíndola, F.; Rodríguez-Gil, J.E.; Concha, I.I.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Genetic diversity analysis in the reproductive nuclei of heavy breeds of the National Equine Promotion Plan by using microsatellite loci,Diversidad genética al interior de los núcleos reproductivos de las razas pesadas del Plan Nacional de Fomento Equino basado en el análisis de loci microsatélites

Archivos de Medicina Veterinaria
2016 | Journal article
EID:

2-s2.0-84969262860

Part of ISBN:

07176201 0301732X

Contributors: Ramírez-Reveco, A.; Hartley, R.; Ortiz, M.; Ulloa, O.; Núñez, I.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Reevaluating the Sperm Nuclear Chromatin Decondensation Test by Sodium Thioglycolate of Stallions Spermatozoa

Journal of Equine Veterinary Science
2016-01 | Journal article
Source: check_circle
Crossref
grade
Preferred source (of 2)‎

Glycolytic potential and activity of adenosine monophosphate kinase (AMPK), glycogen phosphorylase (GP) and glycogen debranching enzyme (GDE) in steer carcasses with normal (&lt;5.8) or high (&gt;5.9) 24h pH determined in M. longissimus dorsi

Meat Science
2015 | Journal article
EID:

2-s2.0-84913534547

Part of ISBN:

03091740

Contributors: Apaoblaza, A.; Galaz, A.; Strobel, P.; Ramírez-Reveco, A.; Jeréz-Timaure, N.; Gallo, C.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Intracellular calcium movements of boar spermatozoa during 'in vitro' capacitation and subsequent acrosome exocytosis follow a multiple-storage place, extracellular calcium-dependent model

Andrology
2015 | Journal article
EID:

2-s2.0-84937518297

Part of ISBN:

20472927 20472919

Contributors: Yeste, M.; Fernández-Novell, J.M.; Ramió-Lluch, L.; Estrada, E.; Rocha, L.G.; Cebrián-Pérez, J.A.; Muiño-Blanco, T.; Concha, I.I.; Ramírez, A.; Rodríguez-Gil, J.E.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

The Wnt1 ligand/Frizzled 3 receptor system plays a regulatory role in the achievement of the 'in vitro' capacitation and subsequent 'in vitro' acrosome exocytosis of porcine spermatozoa

Andrology
2015 | Journal article
EID:

2-s2.0-84928300848

Part of ISBN:

20472927 20472919

Contributors: Covarrubias, A.A.; Yeste, M.; Salazar, E.; Ramírez-Reveco, A.; Rodriguez Gil, J.E.; Concha, I.I.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Water contaminated with Didymosphenia geminata generates changes in Salmo salar spermatozoa activation times

Aquatic Toxicology
2015 | Journal article
EID:

2-s2.0-84928548601

Part of ISBN:

18791514 0166445X

Contributors: Olivares, P.; Orellana, P.; Guerra, G.; Peredo-Parada, M.; Chavez, V.; Ramirez, A.; Parodi, J.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Oligomycin A-induced inhibition of mitochondrial ATP-synthase activity suppresses boar sperm motility and in vitro capacitation achievement without modifying overall sperm energy levels

Reproduction, Fertility and Development
2014 | Journal article
EID:

2-s2.0-84899583421

Part of ISBN:

14485990 10313613

Contributors: Ramió-Lluch, L.; Yeste, M.; Fernández-Novell, J.M.; Estrada, E.; Rocha, L.; Cebrián-Pérez, J.A.; Muiño-Blanco, T.; Concha, I.I.; Ramírez, A.; Rodríguez-Gil, J.E.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Use of hypometabolic TRIS extenders and high cooling rate refrigeration for cryopreservation of stallion sperm: Presence and sensitivity of 5' AMP-activated protein kinase (AMPK)

Cryobiology
2014 | Journal article
EID:

2-s2.0-84922530363

Part of ISBN:

10902392 00112240

Contributors: Córdova, A.; Strobel, P.; Vallejo, A.; Valenzuela, P.; Ulloa, O.; Burgos, R.A.; Menarim, B.; Rodríguez-Gil, J.E.; Ratto, M.; Ramírez-Reveco, A.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Granulocyte-macrophage colony stimulating factor (GM-CSF) enhances cumulus cell expansion in bovine oocytes

Reproductive biology and endocrinology : RB&amp;E
2013 | Journal article
EID:

2-s2.0-84879248512

Part of ISBN:

14777827

Contributors: Peralta, O.A.; Bucher, D.; Fernandez, A.; Berland, M.; Strobel, P.; Ramirez, A.; Ratto, M.H.; Concha, I.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

'In vitro' capacitation and further 'in vitro' progesterone-induced acrosome exocytosis are linked to specific changes in the expression and acrosome location of protein phosphorylation in serine residues of boar spermatozoa

Reproduction in Domestic Animals
2012 | Journal article
EID:

2-s2.0-84865545772

Part of ISBN:

09366768 14390531

Contributors: Ramió-Lluch, L.; Fernández-Novell, J.M.; Peña, A.; Ramírez, A.; Concha, I.I.; Rodríguez-Gil, J.E.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

'In Vitro' Capacitation and Acrosome Reaction are Concomitant with Specific Changes in Mitochondrial Activity in Boar Sperm: Evidence for a Nucleated Mitochondrial Activation and for the Existence of a Capacitation-Sensitive Subpopulational Structure

Reproduction in Domestic Animals
2011 | Journal article
EID:

2-s2.0-79960189789

Part of ISBN:

09366768 14390531

Contributors: Ramió-Lluch, L.; Fernández-Novell, J.M.; Peña, A.; Colás, C.; Cebrián-Pérez, J.A.; Muiño-Blanco, T.; Ramírez, A.; Concha, I.I.; Rigau, T.; Rodríguez-Gil, J.E.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Sperm from Hyh mice carrying a point mutation in αSNAP have a defect in acrosome reaction

PLoS ONE
2009 | Journal article
EID:

2-s2.0-63349098078

Part of ISBN:

19326203

Contributors: Bátiz, L.F.; De Blas, G.A.; Michaut, M.A.; Ramírez, A.R.; Rodríguez, F.; Ratto, M.H.; Oliver, C.; Tomes, C.N.; Rodríguez, E.M.; Mayorga, L.S.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

The presence and function of dopamine type 2 receptors in boar sperm: A possible role for dopamine in viability, capacitation, and modulation of sperm motility

Biology of Reproduction
2009 | Journal article
EID:

2-s2.0-65249165394

Part of ISBN:

00063363 15297268

Contributors: Ramírez, A.R.; Castro, M.A.; Angulo, C.; Ramió, L.; Rivera, M.M.; Torres, M.; Rigau, T.; Rodíguez-Gil, J.E.; Concha, I.I.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Dynamics of motile-sperm subpopulation structure in boar ejaculates subjected to "in vitro" capacitation and further "in vitro" acrosome reaction

Theriogenology
2008 | Journal article
EID:

2-s2.0-38649103781

Part of ISBN:

0093691X

Contributors: Ramió, L.; Rivera, M.M.; Ramírez, A.; Concha, I.I.; Peña, A.; Rigau, T.; Rodríguez-Gil, J.E.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Marlin-1 is expressed in testis and associates to the cytoskeleton and GABA<inf>B</inf> receptors

Journal of Cellular Biochemistry
2008 | Journal article
EID:

2-s2.0-39049100604

Part of ISBN:

07302312 10974644

Contributors: Vidal, R.L.; Ramírez, A.; Castro, M.; Concha, I.I.; Couve, A.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Expression of the GM-CSF receptor in ovine spermatozoa: GM-CSF effect on sperm viability and motility of sperm subpopulations after the freezing-thawing process

Theriogenology
2007 | Journal article
EID:

2-s2.0-34247112362

Part of ISBN:

0093691X

Contributors: Rodríguez-Gil, J.E.; Silvers, G.; Flores, E.; Jesús Palomo, M.; Ramírez, A.; Montserrat Rivera, M.; Castro, M.; Brito, M.; Bücher, D.; Correa, J. et al.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Novel identification of peripheral dopaminergic D2 receptor in male germ cells

Journal of Cellular Biochemistry
2007 | Journal article
EID:

2-s2.0-33845620931

Part of ISBN:

07302312 10974644

Contributors: Otth, C.; Torres, M.; Ramírez, A.; Fernandez, J.C.; Castro, M.; Rauch, M.C.; Brito, M.; Yañez, A.J.; Rodríguez-Gil, J.-E.; Slebe, J.C. et al.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Hexose-specificity of hexokinase and ADP-dependence of pyruvate kinase play important roles in the control of monosaccharide utilization in freshly diluted boar spermatozoa

Molecular Reproduction and Development
2006 | Journal article
EID:

2-s2.0-33746600654

Part of ISBN:

1040452X 10982795

Contributors: Medrano, A.; García-Gil, N.; Ramió, L.; Rivera, Ma.M.; Fernández-Novell, J.Ma.; Ramírez, A.; Peña, A.; Briz, Ma.D.; Pinart, E.; Concha, I.I. et al.
Source: Self-asserted source
Alfredo Ramírez via Scopus - Elsevier

Peer review (3 reviews for 2 publications/grants)

Review activity for Biology of reproduction. (2)
Review activity for Tropical animal health and production. (1)