Thomas B. Jacobs (0000-0002-5408-492X)

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Biography

Thomas obtained his PhD from the University of Georgia with Wayne Parrott, developing several molecular tools for soybean biotechnology. In 2014 he moved to the lab of Greg Martin at the Boyce Thompson Institute for his postdoc. There he started developing CRISPR screens in plants. In 2016 he started the Plant Genome Editing group at the VIB-UGent Center for Plant Systems Biology. In 2022 he was appointed Associate Professor at the University of Gent. His group is focused on developing easy-to-use genome editing systems for all kinds of plant species, specifically focused on making large-scale gene knockouts and base editing.

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Ghent University: Ghent, BE

2022-09-01 to present | Associate Professor (Department of Plant Biotechnology and Bioinformatics)

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Thomas B. Jacobs

https://orcid.org/0000-0002-5408-492X

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Biography

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Employment (5)

Ghent University: Ghent, BE

Vlaams Instituut voor Biotechnologie Department of Plant Systems Biology: Gent, BE

Boyce Thompson Institute for Plant Research: Ithaca, US

University of Georgia: Athens, US

Agrivida (United States): Storry, Connecticut, US

Education and qualifications (3)

University of Georgia: Athens, GA, US

University of Connecticut: Storrs, CT, US

University of Connecticut: Storrs, CT, US

Funding (3)

How Ulva blooms, unravelling the genetic architecture of green tides

Developing technologies to engineer plant genomes at the megabase scale: OMEGA

Development of gRNA design and base editing tools for wheat

Works (43)

Consolidating Ulva functional genomics: gene editing and new selection systems

ConsolidatingUlvafunctional genomics: Gene editing and new selection systems

Continual improvement of CRISPR‐induced multiplex mutagenesis in Arabidopsis

Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts

Phosphoproteome analyses pinpoint the F‐box protein SLOW MOTION as a regulator of warm temperature‐mediated hypocotyl growth in Arabidopsis

Continual improvement of multiplex mutagenesis in Arabidopsis

Combining multiplex gene editing and doubled haploid technology in maize

Ulva: An emerging green seaweed model for systems biology

Identification and characterization of CYP71 subclade cytochrome P450 enzymes involved in the biosynthesis of bitterness compounds in Cichorium intybus

SMAP design: a multiplex PCR amplicon and gRNA design tool to screen for natural and CRISPR-induced genetic variation

Systematic optimization of Cas12a base editors in wheat and maize using the ITER platform

BREEDIT: a multiplex genome editing strategy to improve complex quantitative traits in maize

Protoplast Preparation and Fluorescence-Activated Cell Sorting for the Evaluation of Targeted Mutagenesis in Plant Cells

A Simple and Low‐Tech Heat‐Shock Method to Increase Genome Editing Efficiency in Plants

The heat is on: a simple method to increase genome editing efficiency in plants

Simple and Efficient Modification of Golden Gate Design Standards and Parts Using Oligo Stitching

Optimized Transformation and Gene Editing of the B104 Public Maize Inbred by Improved Tissue Culture and Use of Morphogenic Regulators

Conditional and tissue-specific approaches to dissect essential mechanisms in plant development

ROPGAP-dependent interaction between brassinosteroid and ROP2-GTPase signaling controls pavement cell shape in Arabidopsis

Mini-Review: Transgenerational CRISPR/Cas9 Gene Editing in Plants

Mutually opposing activity of PIN7 splicing isoforms is required for auxin‐mediated tropic responses in Arabidopsis thaliana

A molecular toolkit for the green seaweed Ulva mutabilis

CRISPR screens in plants: approaches, guidelines, and future prospects

Efficient simultaneous mutagenesis of multiple genes in specific plant tissues by multiplex CRISPR

Voices of biotech research

GoldenGateway: A DNA Assembly Method for Plant Biotechnology

Efficient CRISPR-mediated base editing in Agrobacterium spp.

CRISPR-Cas-Mediated Gene Knockout in Tomato

Establishment of CRISPR/Cas9 Genome Editing in Witloof (Cichorium intybus var. foliosum)

A molecular toolkit for the green seaweedUlva mutabilis

Efficient simultaneous mutagenesis of multiple genes in specific plant tissues by multiplex CRISPR

CRISPR-TSKO: A Technique for Efficient Mutagenesis in Specific Cell Types, Tissues, or Organs in Arabidopsis

Genome Editing-Based Engineering of CESA3 Dual Cellulose-Inhibitor-Resistant Plants

DOF2.1 Controls Cytokinin-Dependent Vascular Cell Proliferation Downstream of TMO5/LHW

POLAR-guided signalling complex assembly and localization drive asymmetric cell division

Generation of a Collection of Mutant Tomato Lines Using Pooled CRISPR Libraries

A transgenic, visual screenable marker for soybean seeds

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Simple gene silencing using the trans‐acting si<scp>RNA</scp> pathway

Targeted genome modifications in soybean with CRISPR/Cas9

Exploiting SNPs for biallelic CRISPR mutations in the outcrossing woody perennial Populus reveals 4‐coumarate:CoA ligase specificity and redundancy

Compositions and methods of gene silencing in plants

HtStuf: High‐Throughput Sequencing to Locate Unknown DNA Junction Fragments

Peer review (3 reviews for 3 publications/grants)