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Vavilov Institute of General Genetics Russian Academy of Sciences: Moscow, RU

2006 to present
Employment
Source: Self-asserted source
Konstantin Ignatov

Works (32)

Comparison of Commercially Available Thermostable DNA Polymerases with Reverse Transcriptase Activity in Coupled Reverse Transcription Polymerase Chain Reaction Assays

Methods and Protocols
2025-01-26 | Journal article
Contributors: Evgeniya V. Smirnova; Konstantin A. Blagodatskikh; Ekaterina V. Barsova; Dmitriy A. Varlamov; Vladimir M. Kramarov; Konstantin B. Ignatov
Source: check_circle
Crossref
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Comparison of Commercially Available Thermostable DNA Polymerases with Reverse-Transcriptase Activity in Coupled Reverse-Transcription Polymerase Chain Reaction Assays

2024-12-23 | Preprint
Contributors: Evgeniya V. Smirnova; Konstantin A. Blagodatskikh; Ekaterina V. Barsova; Dmitriy A. Varlamov; Vladimir M. Kramarov; Konstantin B. Ignatov
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Crossref
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Comparison of Commercially Available Thermostable DNA Polymerases with Reverse-Transcriptase Activity in Coupled Reverse-Transcription Polymerase Chain Reaction Assays

2024-08-06 | Preprint
Contributors: Evgeniya V. Smirnova; Konstantin A. Blagodatskikh; Ekaterina V. Barsova; Dmitriy A. Varlamov; Vladimir M. Kramarov; Konstantin B. Ignatov
Source: check_circle
Crossref

Combinations of PCR and Isothermal Amplification Techniques Are Suitable for Fast and Sensitive Detection of SARS-CoV-2 Viral RNA

Frontiers in Bioengineering and Biotechnology
2020-11-04 | Journal article
Part of ISSN: 2296-4185
Source: Self-asserted source
Konstantin Ignatov

Combinations of PCR and isothermal amplification techniques are suitable for fast and sensitive detection of SARS-CoV-2 viral RNA

2020-09 | Preprint
OTHER-ID:

PPR218553

Contributors: Varlamov DA; Blagodatskikh KA; Smirnova EV; Kramarov VMV; Ignatov KB
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central

Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing

2019-04-01 | Journal article
Part of ISSN: 1932-6203
Source: Self-asserted source
Konstantin Ignatov

[Comparative analysis of LAMP and Real Time PCR methods to detect pathogens of glanders and meliodosis.]

Klinicheskaia laboratornaia diagnostika
2018-01 | Journal article
Contributors: Shchit IY; Ignatov KB; Biketov SF
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central

Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA.

PloS one
2017 | Journal article
Contributors: Blagodatskikh KA; Kramarov VM; Barsova EV; Garkovenko AV; Shcherbo DS; Shelenkov AA; Ustinova VV; Tokarenko MR; Baker SC; Kramarova TV et al.
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central
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Preferred source (of 2)‎

The use of loop-mediated isothermal DNA amplification for the detection and identification of the anthrax pathogen

Molecular Genetics Microbiology and Virology
2017 | Journal article
WOSUID:

WOS:000411212600006

Contributors: Shchit, I. Yu.; Ignatov, K. B.; Kudryavtseva, T. Yu.; Shishkova, N. A.; Mironova, R. I.; Marinin, L. I.; Mokrievich, A. N.; Kramarov, V. M.; Biketov, S. F.; Dyatlov, I. A.
Source: Self-asserted source
Konstantin Ignatov via ResearcherID

A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification.

BioTechniques
2014-08 | Journal article
Contributors: Ignatov KB; Barsova EV; Fradkov AF; Blagodatskikh KA; Kramarova TV; Kramarov VM
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central
grade
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DNA ligases from thermophilic bacteria enhance PCR amplification of long DNA sequences.

Biochemistry. Biokhimiia
2009-05 | Journal article
Contributors: Ignatov KB; Kramarov VM
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central
grade
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Cloning and molecular modeling of duodenase with respect to evolution of substrate specificity within mammalian serine proteases that have lost a conserved active-site disulfide bond.

Biochemistry. Biokhimiia
2005-06 | Journal article
Contributors: Zamolodchikova TS; Smirnova EV; Andrianov AN; Kashparov IV; Kotsareva OD; Sokolova EA; Ignatov KB; Pemberton AD
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central
grade
Preferred source (of 2)‎

[A new approach to enhance PCR specificity]

Bioorganicheskaia khimiia
2003 | Journal article
Contributors: Ignatov KB; Miroshnikov AI; Kramarov VM
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central

[A new approach to enhance PCR specificity]

Bioorganicheskaia khimiia
2003 | Journal article
SOURCE-WORK-ID:

0223180922581-5

Contributors: Ignatov KB; Miroshnikov AI; Kramarov VM
Source: Self-asserted source
Konstantin Ignatov via ResearcherID

A new approach to enhanced PCR specificity

Russian Journal of Bioorganic Chemistry
2003 | Journal article
WOSUID:

WOS:000185010300008

Contributors: Ignatov, KB; Miroshnikov, AI; Kramarov, VM
Source: Self-asserted source
Konstantin Ignatov via ResearcherID

Mutation S543N in the thumb subdomain of the Taq DNA polymerase large fragment suppresses pausing associated with the template structure.

FEBS letters
1999-04 | Journal article
Contributors: Ignatov KB; Bashirova AA; Miroshnikov AI; Kramarov VM
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central
grade
Preferred source (of 2)‎

Substitution of Asn for Ser543 in the large fragment of Taq DNA polymerase increases the efficiency of synthesis of long DNA molecules.

FEBS letters
1998-03 | Journal article
PMID: 9559658
Contributors: Ignatov KB; Miroshnikov AI; Kramarov VM
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central
grade
Preferred source (of 2)‎

[DNA polymerase mediated amplification of DNA fragments using primers with mismatches in the 3'-region]

Bioorganicheskaia khimiia
1997 | Journal article
SOURCE-WORK-ID:

0223180922581-8

Contributors: Ignatov KB; Kramarov VM; Uznadze OL; Miroshnikov AI
Source: Self-asserted source
Konstantin Ignatov via ResearcherID

[Integration of IS186 element into the -10-region of the promoter of heat shock lon-gene in Escherichia coli]

Bioorganicheskaia khimiia
1997 | Journal article
SOURCE-WORK-ID:

0223180922581-9

Contributors: Ignatov KB; Chistiakova LG
Source: Self-asserted source
Konstantin Ignatov via ResearcherID

[The factors controlling different activity of DNA-polymerases from Thermus thermophilus and Thermus aquaticus in process of amplification of Phage lambda DNA]

Molekuliarnaia biologiia
1997 | Journal article
PMID: 9480430
Contributors: Ignatov KB; Kramarov VF; Chistiakova LG; Miroshnikov AI
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central

[The factors controlling different activity of DNA-polymerases from Thermus thermophilus and Thermus aquaticus in process of amplification of Phage lambda DNA]

Molekuliarnaia biologiia
1997 | Journal article
SOURCE-WORK-ID:

0223180922581-10

Contributors: Ignatov KB; Kramarov VF; Chistiakova LG; Miroshnikov AI
Source: Self-asserted source
Konstantin Ignatov via ResearcherID

Factors determining different processivity of Thermus thermophilus and T-aquaticus DNA polymerases in amplification of phage lambda DNA

Molecular Biology
1997 | Journal article
WOSUID:

WOS:000072063000004

Contributors: Ignatov, KB; Kramarov, VM; Chistyakova, LG; Miroshnikov, AI
Source: Self-asserted source
Konstantin Ignatov via ResearcherID

Integration of the insertion element IS186 into the -10 region of the heat shock promoter of the Escherichia coli lon gene

Bioorganicheskaya Khimiya
1997 | Journal article
WOSUID:

WOS:A1997WP62400002

Contributors: Ignatov, KB; Chistyakova, LG
Source: Self-asserted source
Konstantin Ignatov via ResearcherID

Tth DNA polymerase-mediated amplification of DNA fragments using primers with mismatches in the 3'-region

Bioorganicheskaya Khimiya
1997 | Journal article
WOSUID:

WOS:A1997YK04300007

Contributors: Ignatov, KB; Kramarov, VM; Uznadze, OL; Miroshnikov, AI
Source: Self-asserted source
Konstantin Ignatov via ResearcherID

[DNA polymerase mediated amplification of DNA fragments using primers with mismatches in the 3'-region]

Bioorganicheskaia khimiia
1997-10 | Journal article
PMID: 9490618
Contributors: Ignatov KB; Kramarov VM; Uznadze OL; Miroshnikov AI
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central

[Integration of IS186 element into the -10-region of the promoter of heat shock lon-gene in Escherichia coli]

Bioorganicheskaia khimiia
1997-01 | Journal article
PMID: 9139638
Contributors: Ignatov KB; Chistiakova LG
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central

[The demonstration of the genes controlling enterotoxigenicity in Staphylococcus aureus strains by using the polymerase chain reaction]

Zhurnal mikrobiologii, epidemiologii, i immunobiologii
1995 | Journal article
PMID: 7778382
Contributors: Grabovetskiĭ VV; Ignatov KB; Chistiakova LG; Diadchenko MA; Vinogradov SV; Kiselev VI
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central

[The demonstration of the genes controlling enterotoxigenicity in Staphylococcus aureus strains by using the polymerase chain reaction]

Zhurnal mikrobiologii, epidemiologii, i immunobiologii
1995 | Journal article
SOURCE-WORK-ID:

0223180922581-11

Contributors: Grabovetskiĭ VV; Ignatov KB; Chistiakova LG; Diadchenko MA; Vinogradov SV; Kiselev VI
Source: Self-asserted source
Konstantin Ignatov via ResearcherID

[Cloning the Staphylococcus aureus enterotoxin B gene, obtained by polymerase chain reaction, and its expression in Escherichia coli cells]

Bioorganicheskaia khimiia
1993 | Journal article
SOURCE-WORK-ID:

0223180922581-12

Contributors: Ignatov KB; Chistiakova LG; Shemchukova OB; Gorodetskaia SB; Kiselev VI
Source: Self-asserted source
Konstantin Ignatov via ResearcherID

CLONING OF THE STAPHYLOCOCCUS-AUREUS ENTEROTOXIN-B GENE BY USING POLYMERASE CHAIN-REACTION AND ITS EXPRESSION IN ESCHERICHIA-COLI-CELLS

Bioorganicheskaya Khimiya
1993 | Journal article
WOSUID:

WOS:A1993LC66300007

Contributors: IGNATOV, KB; CHISTJAKOVA, LG; CHEMCHUKOVA, OB; GORODETSKAYA, SB; KISELEV, VI
Source: Self-asserted source
Konstantin Ignatov via ResearcherID

[Cloning the Staphylococcus aureus enterotoxin B gene, obtained by polymerase chain reaction, and its expression in Escherichia coli cells]

Bioorganicheskaia khimiia
1993-01 | Journal article
PMID: 8484816
Contributors: Ignatov KB; Chistiakova LG; Shemchukova OB; Gorodetskaia SB; Kiselev VI
Source: Self-asserted source
Konstantin Ignatov via Europe PubMed Central

Combinations of PCR and Isothermal Amplification Techniques Are Suitable for Fast and Sensitive Detection of SARS-CoV-2 Viral RNA

Journal article
Source: Self-asserted source
Konstantin Ignatov