PRAKASHA GOWDA ALADAHALLI SANNE GOWDA (0000-0002-0055-7103)

Personal information

Biography

Professional Summary:

Accomplished in biochemistry, chemistry, protein biochemistry, structural biology, biophysics, molecular biology, enzymology, carbohydrate chemistry and glycobiology.

Professional Experience:

Aug 2017 to present: Principal Scientist, Eurofins Lancaster Laboratories, Inc., 2425
New Holland Pike, Lancaster, PA 17601

July 2011-Aug 2017: Research associate, Department of Biochem & Mol Biology,
Penn State University, College of Medicine, 500 University Dr,
Hershey, PA 17033
Discovery of polymerase-specific dNTP: Identified a dNTP
substrate that is specific for only one DNA polymerase, pol κ.
With this discovery will be able to elucidate the functions of pol
κ in a cell.

Oct 2004-June 2011: Postdoctoral Scholar, Department of Bioch & Mol Biology, Penn
State University, College of Medicine, 500 University drive,
Hershey, PA 17033

Jan 2003-June 2004: Postdoctoral Scholar, Department of Biochem & Mol pharma,
University of Massachusetts Medical School, 364 Plantation
Street, LRB, Worcester, MA 01655

April 2002-Jan 2003: Research Associate, Department of Molecular Biophysics Unit,
Indian Institute of Science, Bangalore, Karnataka 560012, India

Nov 1997-Jan 2002: Ph. D. Student: Department of Chemistry, University of Mysore,
Mysore, Karnataka 570006, India

Patent Filed:
PSU Invention Disclosure No. 2016-4445: N2-Substituted 2’-Deoxyguanosine Triphosphate Derivatives as Selective Substrates for Human DNA polymerase k: A. S. Prakasha Gowda and Thomas E. Spratt. Provisional Patent Application Serial No. 62/311,001 [Polymerase Specific Triphosphate Substrate], filed March 21, 2016, and Provisional Patent Application Serial No. 62/361,090, filed July 11, 2016,

Educational Qualification:
2002 Ph.D. Chemistry, University of Mysore, Mysore, India
1997 M. Sc. Biochemistry, University of Mysore, Mysore, India
1991 B. Sc. Biology, University of Mysore, Mysore, India

Publications: 24 (Including Nature, Angewandte Chemie International Edition, JBC, Biochemistry, Chemical research toxicology, European J. of Medicinal chemistry & Cell Death and Disease)

Abstracts/presentations: 30

Research technics expertise:

Biochemical, Biophysical & analytical:
HPLC, Gas chromatography, Densitometer, analytical ultracentrifuge and density gradient centrifugation. Flow cytometry, FACS analysis, Dionex Bio-LC and RQF-3 quenches flow apparatus (Kin Tek). UV Spectrophotometry, Circular Dichroism, Isothermal Titration Calorimeter (ITC). BiAcoreTM[Surface plasmon resonance (SPR) Technology]. FlexStation 3 Multi-Mode (Molecular Devices) and Softmax Pro 5 microplate readers.

Chip Sequence:
• Next generation gene sequencing experiments. DNA sequencing technology
such as MiSeq, HiSeq-2000 and 2500.
• MiSeq/HiSeq result analysis by using Galaxy, Integrative Genomic Viewer (IGV)
and BLAST.

Click Chemistry:
• “Click Reactions” using biotin-conjugated azides and azides
• In vitro and in vivo (live cells) biomolecules labeling by “Click Chemistry”.

Cell Culture:
• cancer, normal, stem cells, Insect cells (SF9 cells), baculovirus, human blood
lymphocytes, mouse embryo fibroblast cells & malarial parasite .

Protein chemistry purification/characterization:
• Protein purification (bacterial, mammalian, insect cells) & characterization. SDS-
PAGE and Immunoblotting. qubit, Nano drop measurements &
plate reader assays.
• Isolation of proteoglycans from human placenta and animal tissue.
• Photo-affinity probe preparation. Radiolabeling of proteins, oligosaccharides & DNA using I125, S35, P32 & H3.

Enzymology:
• Enzyme assays development, Kd, kcat/Km measurements and inhibition studies.
• DNA repair proteins and DNA polymerases, enzyme–DNA binding constants & kinetics of reactions.
• Enzyme pre-steady state, steady state kinetics & single turnover experiments. Fluorescence polarization/anisotropy.

Molecular Biology and Immunology
• Transfection, DNA isolation, western blot, PCR and RT-PCR. Immunofluorescence, immunoprecipitation, ELISA & immunohistochemistry.
DNA ligations, enzyme digestions & DNA shear by Covaris.

Proteomics:
• Mass spectrometry MALDI, LC-MS & MALDI-TOF.

Peptide synthesis and characterization :
• Peptide synthesis, kinetics of peptide analogs to probe the antimicrobial activity.

Mice work: in vivo and in vitro studies.

Activities

Collapse all

Eurofins BioPharma Product Testing: Lancaster, PA, US

2017-09-01 to present | Principal Scientist (Department of raw materials)

Employment

PRAKASHA GOWDA ALADAHALLI SANNE GOWDA

https://orcid.org/0000-0002-0055-7103

Personal information

Websites & social links

Keywords

Countries

Biography

Activities

Employment (1)

Eurofins BioPharma Product Testing: Lancaster, PA, US

Education and qualifications (1)

University of Mysore: Mysore, Karnataka, IN

Works (24)

Effect of Excipients on Recombinant Interleukin-2 Stability in Aqueous Buffers

A simple RP-HPLC method for the stability-indicating determination of N-acetyl-L-cysteine and N,N’-diacetyl-L-cystine in cell culture media

Active Site Interactions Impact Phosphoryl Transfer during Replication of Damaged and Undamaged DNA by Escherichia coli DNA Polymerase I

Design, synthesis, and identification of a novel napthalamide-isoselenocyanate compound NISC-6 as a dual Topoisomerase-IIα and Akt pathway inhibitor, and evaluation of its anti-melanoma activity

Honokiol Inhibits DNA Polymerases β and λ and Increases Bleomycin Sensitivity of Human Cancer Cells

N2-Substituted 2′-Deoxyguanosine Triphosphate Derivatives as Selective Substrates for Human DNA Polymerase κ

DNA Polymerase ν Rapidly Bypasses O6-Methyl-dG but Not O6-[4-(3-Pyridyl)-4-oxobutyl-dG and O2-Alkyl-dTs

DNA Polymerases η and ζ Combine to Bypass O2-[4-(3-Pyridyl)-4-oxobutyl]thymine, a DNA Adduct Formed from Tobacco Carcinogens

Human DNA Polymerase ν Catalyzes Correct and Incorrect DNA Synthesis with High Catalytic Efficiency

Interaction of Sirt3 with OGG1 contributes to repair of mitochondrial DNA and protects from apoptotic cell death under oxidative stress

In vitro growth inhibition of human cancer cells by novel honokiol analogs

Low Fidelity Bypass of O2-(3-Pyridyl)-4-oxobutylthymine, the Most Persistent Bulky Adduct Produced by the Tobacco Specific Nitrosamine 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone by Model DNA Polymerases

Synthesis and biological evaluation of a novel class of isatin analogs as dual inhibitors of tubulin polymerization and Akt pathway

Development of novel naphthalimide derivatives and their evaluation as potential melanoma therapeutics

Facile Syntheses ofO2-[4-(3-Pyridyl-4-oxobut-1-yl]thymidine, the Major Adduct Formed by Tobacco Specific Nitrosamine 4-methylnitrosamino-1-(3-pyridyl)-1-butanone (NNK)in vivo, and Its Site-Specifically Adducted Oligodeoxynucleotides

An unprecedented nucleic acid capture mechanism for excision of DNA damage

Incorporation of Gemcitabine and Cytarabine into DNA by DNA Polymerase β and Ligase III/XRCC1

Structural Basis for the Adherence of Plasmodium falciparum-infected Erythrocytes to Chondroitin 4-Sulfate and Design of Novel Photoactivable Reagents for the Identification of Parasite Adhesive Proteins

Plasmepsin inhibitors: design, synthesis, inhibitory studies and crystal structure analysis

Design, Synthesis and Antibacterial Activity Studies of Model Peptides of Proline / Arginine-Rich Region in Bactenecin7

Selective Antifungal Activity of Shorter Active Analogues of Bactenecin7 Against Fusarium moniliforme.

Synthesis of shorter active analogues of bactenecin7: The effect of change ofN-terminal configuration on antimicrobial activity

Nickel-Catalyzed Formic Acid Reductions. A Selective Method for the Reduction of Nitro Compounds

Mutagenic Replication of N2Deoxyguanosine Benzo[a]pyrene Adducts by Escherichia coli DNA Polymerase I and Sulfolobus solfataricus DNA Polymerase IV