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Hien P. Nguyen is among a group of international colleagues who are together working on molecular plant-microbe interactions towards efficient biological symbiotic Nitrogen fixation. His works mainly focus on: (1) characterization of molecular mechanisms underlying compatible/incompatible interactions between legumes (Vigna species and G. max) and rhizobia, using multidisciplinary approaches (collaborated with the Japanese group at Tokyo University of Agriculture and Technology, TUAT), (2) computational analysis of diversity and evolution of horizontally transferred symbiosis islands and Type III secretion system (T3SS) from chickpea Mesorhizobium symbionts (collaborated with an American group at The University of California, Davis - UCD), (3) biochemical and genetic identification of interaction partners of rhizobial T3 effectors (collaborated with a German group at Dresden University of Technology - TU Dresden, and Thai group at Suranaree University of Technology - SUT), and (4) novel rhizobial strains exhibiting superior nodulation on soybean under high nitrate conditions (collaborated with the Japanese groups).