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Kyoto Univ. Activity DB

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Researcher Name Resolver ID: 1000070553535

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Employment (4)

京都大学 / Kyoto University: 京都市左京区 / Sakyo, Kyoto, JP

2018-04-01 to present | 特定准教授 / Program-Specific Associate Professor (農学研究科 / Graduate Schools of Agriculture)
Employment
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Kyoto University

Kyoto University: Kyoto, JP

2018-04-01 to present (Graduate School of Agriculture)
Employment
Source: Self-asserted source
Ryotaro Hara

Waseda University: Tokyo, JP

2011-04-01 to 2018-03-31 (Research Institute for Science and Engineering)
Employment
Source: Self-asserted source
Ryotaro Hara

Waseda University: Tokyo, JP

2009-04-01 to 2011-03-31 (Faculty of Science and Engineering)
Employment
Source: Self-asserted source
Ryotaro Hara

Education and qualifications (3)

Waseda University: Tokyo, JP

2006-04-01 to 2009-03-15 | Doctor of Engineering (Department of Applied Chemisty, Graduate School of Science and Engineerin)
Education
Source: Self-asserted source
Ryotaro Hara

Waseda University: Tokyo, JP

2004-04-01 to 2006-03-15 | Master of Engineering (Department of Applied Chemisty, Graduate School of Science and Engineering)
Education
Source: Self-asserted source
Ryotaro Hara

Waseda University: Tokyo, JP

2000-04-01 to 2004-03-15 | Bachelor of Engineering (Department of Applied Chemisty, School of Science and Engineering)
Education
Source: Self-asserted source
Ryotaro Hara

Works (24)

Identification of Two Distinct Stereoselective Lysine 5‐Hydroxylases by Genome Mining Based on Alazopeptin Biosynthetic Enzymes

Chemistry – A European Journal
2025-02-28 | Journal article
DOI: 10.1002/chem.202404790
Contributors: Seiji Kawai; Kota Moriga; Warawadee Nirdnoy; Ryotaro Hara; Jun Ogawa; Yohei Katsuyama; Yasuo Ohnishi
Source: check_circle
Crossref

Identification of tryptophanase from Escherichia coli for the synthesis of S-allyl-l-cysteine and related S-substituted cysteine derivatives

Journal of Bioscience and Bioengineering
2022-09 | Journal article
DOI: 10.1016/j.jbiosc.2022.06.001
Contributors: Taku Mizutani; Ryotaro Hara; Takayuki Iihoshi; Shoko Kozono; Michiki Takeuchi; Makoto Hibi; Satomi Takahashi; Makoto Ueda; Jun Ogawa
Source: check_circle
Crossref

Enzymatic Synthesis of l - threo -β-Hydroxy-α-Amino Acids via Asymmetric Hydroxylation Using 2-Oxoglutarate-Dependent Hydroxylase from Sulfobacillus thermotolerans Strain Y0017

Applied and Environmental Microbiology
2021-09-28 | Journal article
DOI: 10.1128/AEM.01335-21
Contributors: Ryotaro Hara; Yuta Nakajima; Hiroaki Yanagawa; Ryo Gawasawa; Izumi Hirasawa; Kuniki Kino; Haruyuki Atomi
Source: check_circle
Crossref

Screening, gene cloning, and characterization of orsellinic acid decarboxylase from Arthrobacter sp. K8 for regio-selective carboxylation of resorcinol derivatives

Journal of Biotechnology
2020-11 | Journal article
DOI: 10.1016/j.jbiotec.2020.08.011
Contributors: Kuniki Kino; Yasutaka Hirokawa; Ryo Gawasawa; Ryota Murase; Ryohei Tsuchihashi; Ryotaro Hara
Source: check_circle
Crossref

Enzymatic reactions and microorganisms producing the various isomers of hydroxyproline

Applied Microbiology and Biotechnology
2020-06-15 | Journal article
DOI: 10.1007/s00253-020-10603-1
Contributors: Ryotaro Hara; Kuniki Kino
Source: check_circle
Crossref

Synthesis of d -Amino Acid-Containing Dipeptides Using the Adenylation Domains of Nonribosomal Peptide Synthetase

Applied and Environmental Microbiology
2019-07 | Journal article
DOI: 10.1128/AEM.00120-19
Contributors: Soichiro Kano; Shin Suzuki; Ryotaro Hara; Kuniki Kino; Claire Vieille
Source: check_circle
Crossref

Ectoine hydroxylase displays selective trans-3-hydroxylation activity towards l-proline

Applied Microbiology and Biotechnology
2019-07-20 | Journal article
DOI: 10.1007/s00253-019-09868-y
Contributors: Ryotaro Hara; Takeyuki Nishikawa; Takuya Okuhara; Kento Koketsu; Kuniki Kino
Source: check_circle
Crossref

A chemoenzymatic process for amide bond formation by an adenylating enzyme-mediated mechanism

Scientific Reports
2018-12 | Journal article
DOI: 10.1038/s41598-018-21408-8
Part of ISSN: 2045-2322
Source: Self-asserted source
Ryotaro Hara

Production of aminoacyl prolines using the adenylation domain of nonribosomal peptide synthetase with class III polyphosphate kinase 2-mediated ATP regeneration

Journal of Bioscience and Bioengineering
2018-06 | Journal article
DOI: 10.1016/j.jbiosc.2017.12.023
Contributors: Shin Suzuki; Ryotaro Hara; Kuniki Kino
Source: check_circle
Crossref

Discovery of Lysine Hydroxylases in the Clavaminic Acid Synthase-Like Superfamily for Efficient Hydroxylysine Bioproduction

Applied and Environmental Microbiology
2017-09-01 | Journal article
DOI: 10.1128/aem.00693-17
Part of ISSN: 0099-2240
Part of ISSN: 1098-5336
Source: Self-asserted source
Ryotaro Hara

Functional characterization of aconitase X as a cis-3-hydroxy-L-proline dehydratase

Scientific Reports
2016-12 | Journal article
DOI: 10.1038/srep38720
Part of ISSN: 2045-2322
Source: Self-asserted source
Ryotaro Hara

Development of a multi-enzymatic cascade reaction for the synthesis of trans-3-hydroxy-l-proline from l-arginine

Applied Microbiology and Biotechnology
2016-01 | Journal article
DOI: 10.1007/s00253-015-6992-4
Part of ISSN: 0175-7598
Part of ISSN: 1432-0614
Source: Self-asserted source
Ryotaro Hara

One-Pot Production of l-threo-3-Hydroxyaspartic Acid Using Asparaginase-Deficient Escherichia coli Expressing Asparagine Hydroxylase of Streptomyces coelicolor A3(2)

Applied and Environmental Microbiology
2015-06-01 | Journal article
DOI: 10.1128/aem.03963-14
Part of ISSN: 0099-2240
Part of ISSN: 1098-5336
Source: Self-asserted source
Ryotaro Hara

Hydroxamate-based colorimetric assay to assess amide bond formation by adenylation domain of nonribosomal peptide synthetases

Analytical Biochemistry
2015-05 | Journal article
DOI: 10.1016/j.ab.2015.01.006
Part of ISSN: 0003-2697
Source: Self-asserted source
Ryotaro Hara

Refined Regio- and Stereoselective Hydroxylation of l-Pipecolic Acid by Protein Engineering of l-Proline cis-4-Hydroxylase Based on the X-ray Crystal Structure

ACS Synthetic Biology
2015-04-17 | Journal article
DOI: 10.1021/sb500247a
Part of ISSN: 2161-5063
Part of ISSN: 2161-5063
Source: Self-asserted source
Ryotaro Hara

Regio- and stereoselective oxygenation of proline derivatives by using microbial 2-oxoglutarate-dependent dioxygenases

Bioscience, Biotechnology, and Biochemistry
2014-08-03 | Journal article
DOI: 10.1080/09168451.2014.918490
Part of ISSN: 0916-8451
Part of ISSN: 1347-6947
Source: Self-asserted source
Ryotaro Hara

Identification and characterization of 2-oxoglutarate-dependent dioxygenases catalyzing selective cis-hydroxylation of proline and pipecolinic acid from actinomycetes

Journal of Biotechnology
2014-02 | Journal article
DOI: 10.1016/j.jbiotec.2013.12.003
Part of ISSN: 0168-1656
Source: Self-asserted source
Ryotaro Hara

Enhanced synthesis of 5-hydroxy-l-tryptophan through tetrahydropterin regeneration

AMB Express
2013 | Journal article
DOI: 10.1186/2191-0855-3-70
Part of ISSN: 2191-0855
Source: Self-asserted source
Ryotaro Hara

Microbial production of N-acetyl cis-4-hydroxy-l-proline by coexpression of the Rhizobium l-proline cis-4-hydroxylase and the yeast N-acetyltransferase Mpr1

Applied Microbiology and Biotechnology
2013-01 | Journal article
DOI: 10.1007/s00253-012-4204-z
Part of ISSN: 0175-7598
Part of ISSN: 1432-0614
Source: Self-asserted source
Ryotaro Hara

Industrial Production of threo-3-Hydroxy-L-Aspartic Acid Using Escherichia coli Resting Cells

Journal of Biotechnology
2010-11 | Journal article
DOI: 10.1016/j.jbiotec.2010.09.450
Part of ISSN: 0168-1656
Source: Self-asserted source
Ryotaro Hara

Enhancement of l-tryptophan 5-hydroxylation activity by structure-based modification of l-phenylalanine 4-hydroxylase from Chromobacterium violaceum

Journal of Bioscience and Bioengineering
2009-09 | Journal article
DOI: 10.1016/j.jbiosc.2009.04.002
Part of ISSN: 1389-1723
Source: Self-asserted source
Ryotaro Hara

Characterization of novel 2-oxoglutarate dependent dioxygenases converting l-proline to cis-4-hydroxy-l-proline

Biochemical and Biophysical Research Communications
2009-02 | Journal article
DOI: 10.1016/j.bbrc.2008.12.158
Part of ISSN: 0006-291X
Source: Self-asserted source
Ryotaro Hara

Two groups of thermophilic amino acid aminotransferases exhibiting broad substrate specificities for the synthesis of phenylglycine derivatives

Applied Microbiology and Biotechnology
2008-07 | Journal article
DOI: 10.1007/s00253-008-1487-1
Part of ISSN: 0175-7598
Part of ISSN: 1432-0614
Source: Self-asserted source
Ryotaro Hara

A high-throughput and generic assay method for the determination of substrate specificities of thermophilic α-aminotransferases

Journal of Microbiological Methods
2007-10 | Journal article
DOI: 10.1016/j.mimet.2007.07.006
Part of ISSN: 0167-7012
Source: Self-asserted source
Ryotaro Hara

Peer review (4 reviews for 3 publications/grants)

Review activity for Biotechnology letters. (1)
Review activity for Food chemistry. (2)
Review activity for Journal of bioscience and bioengineering. (1)
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