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Other IDs

SciProfiles: 981492
Loop profile: 842657
Scopus Author ID: 57214561135

Keywords

CRISPR, RNAi, genetic perturbation, pooled screens, cancer

Countries

Germany

Activities

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Employment (5)

Martin Luther University Halle-Wittenberg: Halle, DE

2019-04-01 to present | Juniorprofessor (Molecular Medicine of Signal Transduction)
Employment
Source: Self-asserted source
Michael Boettcher

Max Planck Institute for Infection Biology: Berlin, DE

2018-10-01 to 2019-03-31 | Head of Genome Engineering (Charpentier lab)
Employment
Source: Self-asserted source
Michael Boettcher

Max Planck Institute for Molecular Genetics: Berlin, DE

2017-01-01 to 2018-09-30 | Senior Postdoctoral Researcher (Lehrach lab)
Employment
Source: Self-asserted source
Michael Boettcher

University of California San Francisco: San Francisco, CA, US

2014-01-01 to 2016-12-31 | Postdoctoral Researcher (McManus lab)
Employment
Source: Self-asserted source
Michael Boettcher

Deutsches Krebsforschungszentrum: Heidelberg, Baden-Württemberg, DE

2010-04-01 to 2012-12-31 | Postdoctoral Researcher (Hoheisel lab)
Employment
Source: Self-asserted source
Michael Boettcher

Education and qualifications (3)

Deutsches Krebsforschungszentrum: Heidelberg, Baden-Württemberg, DE

2006-10-01 to 2010-04-31 | PhD in Molecular Cancer Biology (Functional Genome Analysis)
Education
Source: Self-asserted source
Michael Boettcher

Australian Centre for Plant Functional Genomics: Glen Osmond, SA, AU

2004-11-01 to 2005-07-31 | Diplom thesis
Education
Source: Self-asserted source
Michael Boettcher

University of Freiburg: Freiburg, Baden-Württemberg, DE

1999-10-01 to 2005-07-31 | Biology Diplom
Education
Source: Self-asserted source
Michael Boettcher

Works (29)

Evaluation of Cas13d as a tool for genetic interaction mapping.

Nature communications
2025-02-14 | Journal article | Author
DOI: 10.1038/s41467-025-56747-4
PMID: 39952934
Contributors: El Kassem G; Hillmer J; Michael Boettcher
Source: Self-asserted source
Michael Boettcher

Genome-Wide CRISPR Screen Identifies Genes Involved in Metastasis of Pancreatic Ductal Adenocarcinoma

Cancers
2024-10 | Journal article | Author
DOI: 10.3390/cancers16213684
Contributors: Risky Oktriani; anna chiara pirona; Lili Kalmár; Ariani Rahadian; Beiping Miao; Andrea S. Bauer; Jörg Hoheisel; Michael Boettcher; Haoqi Du
Source: check_circle
Multidisciplinary Digital Publishing Institute

Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK Response

2024-01-15 | Preprint | Author
DOI: 10.1101/2024.01.13.575500
Contributors: Ghanem El Kassem; Anja Sieber; Bertram Klinger; Florian Uhlitz; David Steinbrecht; Mirjam van Bentum; Jasmine Hillmer; Jennifer von Schlichting; Reinhold Schäfer; Nils Blüthgen et al.
Source: Self-asserted source
Michael Boettcher

Mutations in ALK signaling pathways conferring resistance to ALK inhibitor treatment lead to collateral vulnerabilities in neuroblastoma cells.

Molecular cancer
2022-06-10 | Journal article
DOI: 10.1186/s12943-022-01583-z
PMID: 35689207
Source: Self-asserted source
Michael Boettcher
grade
Preferred source (of 2)‎

Identification of X-chromosomal genes that drive sex differences in embryonic stem cells through a hierarchical CRISPR screening approach.

Genome biology
2021-04-16 | Journal article
DOI: 10.1186/s13059-021-02321-2
PMID: 33863351
Source: Self-asserted source
Michael Boettcher
grade
Preferred source (of 2)‎

High-Throughput CRISPR Screening Identifies Genes Involved in Macrophage Viability and Inflammatory Pathways.

Cell reports
2020-12-01 | Journal article
DOI: 10.1016/j.celrep.2020.108541
PMID: 33378675
Source: Self-asserted source
Michael Boettcher
grade
Preferred source (of 2)‎

Bridge helix arginines play a critical role in Cas9 sensitivity to mismatches.

Nature chemical biology
2020-03-02 | Journal article
DOI: 10.1038/s41589-020-0490-4
PMID: 32123387
Source: Self-asserted source
Michael Boettcher
grade
Preferred source (of 2)‎

Process for an efficient lentiviral cell transduction

Biology Methods and Protocols
2020-03-02 | Journal article
DOI: 10.1093/biomethods/bpaa005
Part of ISSN: 2396-8923
Source: Self-asserted source
Michael Boettcher
grade
Preferred source (of 2)‎

Host-Receptor Post-Translational Modifications Refine Staphylococcal Leukocidin Cytotoxicity.

Toxins
2020-02-06 | Journal article
DOI: 10.3390/toxins12020106
PMID: 32041354
Source: Self-asserted source
Michael Boettcher
grade
Preferred source (of 2)‎

High throughput CRISPR screening identifies genes involved in macrophage viability and inflammatory pathways

bioRxiv
2019 | Other
DOI: 10.1101/807164
EID:

2-s2.0-85095656817

Part of ISSN: 26928205
Contributors: Covarrubias, S.; Vollmers, A.; Capili, A.; Boettcher, M.; Robinson, E.K.; O’Briain, L.; Vollmers, C.; Blau, J.; McManus, M.; Carpenter, S.
Source: Self-asserted source
Michael Boettcher via Scopus - Elsevier

Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding.

BMC genomics
2019-02 | Journal article
DOI: 10.1186/s12864-019-5480-0
PMID: 30727954
Contributors: Boettcher M; Covarrubias S; Biton A; Blau J; Wang H; Zaitlen N; McManus MT
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

Erratum to: Human CD45 is an F-component-specific receptor for the staphylococcal toxin Panton–Valentine leukocidin (Nature Microbiology, (2018), 3, 6, (708-717), 10.1038/s41564-018-0159-x)

Nature Microbiology
2018 | Journal article
DOI: 10.1038/s41564-018-0247-y
EID:

2-s2.0-85053341440

Part of ISSN: 20585276
Contributors: Tromp, A.T.; Van Gent, M.; Abrial, P.; Martin, A.; Jansen, J.P.; De Haas, C.J.C.; Van Kessel, K.P.M.; Bardoel, B.W.; Kruse, E.; Bourdonnay, E. et al.
Source: Self-asserted source
Michael Boettcher via Scopus - Elsevier

Human CD45 is an F-component-specific receptor for the staphylococcal toxin Panton-Valentine leukocidin.

Nature microbiology
2018-05 | Journal article
DOI: 10.1038/s41564-018-0159-x
PMID: 29736038
Contributors: Tromp AT; Van Gent M; Abrial P; Martin A; Jansen JP; De Haas CJC; Van Kessel KPM; Bardoel BW; Kruse E; Bourdonnay E et al.
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

Genome-wide CRISPR screen identifies FAM49B as a key regulator of actin dynamics and T cell activation.

Proceedings of the National Academy of Sciences of the United States of America
2018-04 | Journal article
DOI: 10.1073/pnas.1801340115
PMID: 29632189
Contributors: Shang W; Jiang Y; Boettcher M; Ding K; Mollenauer M; Liu Z; Wen X; Liu C; Hao P; Zhao S et al.
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

Dual gene activation and knockout screen reveals directional dependencies in genetic networks.

Nature biotechnology
2018-01 | Journal article
DOI: 10.1038/nbt.4062
PMID: 29334369
Contributors: Boettcher M; Tian R; Blau JA; Markegard E; Wagner RT; Wu D; Mo X; Biton A; Zaitlen N; Fu H et al.
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

Decoding directional genetic dependencies through orthogonal CRISPR/Cas screens

bioRxiv
2017 | Other
DOI: 10.1101/120170
EID:

2-s2.0-85095628509

Part of ISSN: 26928205
Contributors: Boettcher, M.; Tian, R.; Blau, J.; Markegard, E.; Wu, D.; Biton, A.; Zaitlen, N.; McCormick, F.; Kampmann, M.; McManus, M.T.
Source: Self-asserted source
Michael Boettcher via Scopus - Elsevier

Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding

bioRxiv
2017 | Other
DOI: 10.1101/224345
EID:

2-s2.0-85095644810

Part of ISSN: 26928205
Contributors: Boettcher, M.; Covarrubias, S.; Biton, A.; Blau, J.A.; Wang, H.; Zaitlen, N.; McManus, M.T.
Source: Self-asserted source
Michael Boettcher via Scopus - Elsevier

Dissection of CD20 regulation in lymphoma using RNAi.

Leukemia
2016-12 | Journal article
DOI: 10.1038/leu.2016.230
PMID: 27560109
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central

Transforming Big Data into Cancer-Relevant Insight: An Initial, Multi-Tier Approach to Assess Reproducibility and Relevance.

Molecular cancer research : MCR
2016-08 | Journal article
DOI: 10.1158/1541-7786.mcr-16-0090
PMID: 27401613
PMC: PMC4987219
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central

Choosing the Right Tool for the Job: RNAi, TALEN, or CRISPR.

Molecular cell
2015-05 | Journal article
DOI: 10.1016/j.molcel.2015.04.028
PMID: 26000843
PMC: PMC4441801
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

An in vivo RNAi screen identifies SALL1 as a tumor suppressor in human breast cancer with a role in CDH1 regulation.

Oncogene
2014-08 | Journal article
DOI: 10.1038/onc.2013.515
PMID: 24292671
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

High throughput synthetic lethality screen reveals a tumorigenic role of adenylate cyclase in fumarate hydratase-deficient cancer cells.

BMC genomics
2014-02 | Journal article
DOI: 10.1186/1471-2164-15-158
PMID: 24568598
PMC: PMC3945041
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

A mammosphere formation RNAi screen reveals that ATG4A promotes a breast cancer stem-like phenotype.

Breast cancer research : BCR
2013-11 | Journal article
DOI: 10.1186/bcr3576
PMID: 24229464
PMC: PMC3978845
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

Depletion of RAD17 sensitizes pancreatic cancer cells to gemcitabine.

Journal of cell science
2013-08 | Journal article
DOI: 10.1242/jcs.124768
PMID: 23687379
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

ER-alpha-cDNA as part of a bicistronic transcript gives rise to high frequency, long term, receptor expressing cell clones.

PLoS ONE
2012 | Journal article
DOI: 10.1371/journal.pone.0031977
PMID: 22363779
PMC: PMC3282794
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

Establishment and characterization of a highly tumourigenic and cancer stem cell enriched pancreatic cancer cell line as a well defined model system.

PloS one
2012 | Journal article
DOI: 10.1371/journal.pone.0048503
PMID: 23152778
PMC: PMC3495919
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

Decoding pooled RNAi screens by means of barcode tiling arrays.

BMC genomics
2010 | Journal article
DOI: 10.1186/1471-2164-11-7
PMID: 20051122
PMC: PMC2824726
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

High-definition DNA methylation profiles from breast and ovarian carcinoma cell lines with differing doxorubicin resistance.

PloS one
2010 | Journal article
DOI: 10.1371/journal.pone.0011002
PMID: 20544021
PMC: PMC2882327
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎

Pooled RNAi Screens - Technical and Biological Aspects.

Current Genomics
2010-05 | Journal article
DOI: 10.2174/138920210791110988
PMID: 21037854
PMC: PMC2878981
Source: Self-asserted source
Michael Boettcher via Europe PubMed Central
grade
Preferred source (of 2)‎
How many people are using ORCID?