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University of Cambridge: Cambridge, Cambridgeshire, GB

2001-10-01 to present | Professor of Chemical and Synthetic Biology (Biochemistry)
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Source: Self-asserted source
Florian Hollfelder

Works (50 of 181)

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Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets

Journal of the American Chemical Society
2025-03-24 | Journal article
DOI: 10.1021/jacs.4c11804
Contributors: Matthew Penner; Oskar James Klein; Maximilian Gantz; Friederike E. H. Nintzel; Anne-Cathrin Prowald; Sally Boss; Paul Barker; Paul Dupree; Florian Hollfelder
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Crossref

On synergy between ultrahigh throughput screening and machine learning in biocatalyst engineering

Faraday Discussions
2024 | Journal article
DOI: 10.1039/D4FD00065J
Contributors: Maximilian Gantz; Simon V. Mathis; Friederike E. H. Nintzel; Pietro Lio; Florian Hollfelder
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Peptide dendrimers transfecting CRISPR/Cas9 plasmid DNA: optimization and mechanism

RSC Chemical Biology
2024 | Journal article
DOI: 10.1039/D4CB00116H
Contributors: Susanna Zamolo; Elena Zakharova; Lise Boursinhac; Florian Hollfelder; Tamis Darbre; Jean-Louis Reymond
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Crossref

Expanding the repertoire of imine reductases by mining divergent biosynthetic pathways for promiscuous reactivity

Chem Catalysis
2024-12 | Journal article
DOI: 10.1016/j.checat.2024.101160
Contributors: Godwin A. Aleku; Florian Hollfelder
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Crossref

Pre-oviposition development of the brown anole (Anolis sagrei)

2024-12-17 | Preprint
DOI: 10.1101/2024.12.15.628549
Contributors: Antonia Weberling; Natalia A. Shylo; Bonnie K. Kircher; Hannah Wilson; Melainia McClain; Marta Marchini; Katherine Starr; Thomas J. Sanger; Florian Hollfelder; Paul Trainor
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Ancestral aryl sulfatases in the alkaline phosphatase family allow for greater increases in catalytic efficiency than extant variants

2024-12-11 | Preprint
DOI: 10.1101/2024.12.10.627700
Contributors: Bernard Eenink; Magdalena Heberlein; Josephin M. Holstein; Andreas Lange; Carina Dilkaute; Tomasz S. Kaminski; Erich Bornberg-Bauer; Joachim Jose; Florian Hollfelder; Bert van Loo
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Crossref

Selection of a promiscuous minimalist cAMP phosphodiesterase from a library of de novo designed proteins

Nature Chemistry
2024-07 | Journal article
DOI: 10.1038/s41557-024-01490-4
Contributors: J. David Schnettler; Michael S. Wang; Maximilian Gantz; H. Adrian Bunzel; Christina Karas; Florian Hollfelder; Michael H. Hecht
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Crossref

Ultrahigh Throughput Evolution of Tryptophan Synthase in Droplets via an Aptamer Sensor

ACS Catalysis
2024-04-19 | Journal article
DOI: 10.1021/acscatal.4c00230
Contributors: Remkes A. Scheele; Yanik Weber; Friederike E. H. Nintzel; Michael Herger; Tomasz S. Kaminski; Florian Hollfelder
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Crossref

Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance

Lab on a Chip
2023 | Journal article
DOI: 10.1039/D2LC00871H
Contributors: Esther S. Richter; Andreas Link; John S. McGrath; Raymond W. Sparrow; Maximilian Gantz; Elliot J. Medcalf; Florian Hollfelder; Thomas Franke
Source: check_circle
Crossref

The biosynthesis, degradation, and function of cell wall β‐xylosylated xyloglucan mirrors that of arabinoxyloglucan

New Phytologist
2023-12 | Journal article
DOI: 10.1111/nph.19305
Contributors: Louis F. L. Wilson; Stefanie Neun; Li Yu; Theodora Tryfona; Katherine Stott; Florian Hollfelder; Paul Dupree
Source: check_circle
Crossref

Fluorogenic, sub-single-turnover monitoring of enzymatic reactions involving NAD(P)H provides a generalised platform for directed ultrahigh throughput evolution of biocatalysts in microdroplets

2023-11-22 | Preprint
DOI: 10.1101/2023.11.22.568356
Contributors: Matthew Penner; Oskar James Klein; Maximilian Gantz; Friederike E. H. Nintzel; Anne-Cathrin Prowald; Sally Boss; Paul Barker; Paul Dupree; Florian Hollfelder
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Crossref

YeastIT: Reducing mutational bias for in vivo directed evolution using a novel yeast mutator strain based on dual adenine-/cytosine-targeting and error-prone DNA repair

2023-11-20 | Preprint
DOI: 10.1101/2023.11.20.567881
Contributors: Marta Napiorkowska; Katrin Fischer; Matthew Penner; Philipp Knyphausen; F. Hollfelder
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Ultrahigh throughput evolution of tryptophan synthase in droplets via an aptamer-biosensor

2023-10-12 | Preprint
DOI: 10.1101/2023.10.11.561886
Contributors: Remkes A. Scheele; Yanik Weber; Friederike E. H. Nintzel; Michael Herger; Tomasz S. Kaminski; Florian Hollfelder
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Chemoenzymatic Photoreforming: A Sustainable Approach for Solar Fuel Generation from Plastic Feedstocks

Journal of the American Chemical Society
2023-09-20 | Journal article
DOI: 10.1021/jacs.3c05486
Contributors: Subhajit Bhattacharjee; Chengzhi Guo; Erwin Lam; Josephin M. Holstein; Mariana Rangel Pereira; Christian M. Pichler; Chanon Pornrungroj; Motiar Rahaman; Taylor Uekert; Florian Hollfelder et al.
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Versatile Product Detection via Coupled Assays for Ultrahigh-Throughput Screening of Carbohydrate-Active Enzymes in Microfluidic Droplets

ACS Catalysis
2023-08-04 | Journal article
DOI: 10.1021/acscatal.3c01609
Contributors: Simon Ladeveze; Paul J. Zurek; Tomasz S. Kaminski; Stephane Emond; Florian Hollfelder
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Author Correction: Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension

Nature Cell Biology
2023-06 | Journal article
DOI: 10.1038/s41556-023-01157-1
Contributors: Min Bao; Jake Cornwall-Scoones; Estefania Sanchez-Vasquez; Andy L. Cox; Dong-Yuan Chen; Joachim De Jonghe; Shahriar Shadkhoo; Florian Hollfelder; Matt Thomson; David M. Glover et al.
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Expansion of novel biosynthetic gene clusters from diverse environments using SanntiS

2023-05-24 | Preprint
DOI: 10.1101/2023.05.23.540769
Contributors: Santiago Sanchez; Joel D. Rogers; Alexander B. Rogers; Maaly Nassar; Johanna McEntyre; Martin Welch; Florian Hollfelder; Robert D. Finn
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Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

Chemical Reviews
2023-05-10 | Journal article
DOI: 10.1021/acs.chemrev.2c00910
Contributors: Maximilian Gantz; Stefanie Neun; Elliot J. Medcalf; Liisa D. van Vliet; Florian Hollfelder
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Rapid quantitative assessment of small molecule leakage from microdroplets by flow cytometry and improvement of fluorophore retention in biochemical assays

2023-04-23 | Preprint
DOI: 10.1101/2023.04.23.538007
Contributors: Anastasia Zinchenko; Sean R. A. Devenish; Florian Hollfelder
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Crossref

Versatile Product Detection via Coupled Assays for Ultra-high-throughput Screening of Carbohydrate-Active-Enzymes in Microfluidic Droplets

2023-03-29 | Preprint
DOI: 10.1101/2023.03.29.534725
Contributors: Simon Ladeveze; Paul J. Zurek; Tomasz S. Kaminski; Stephane Emond; Florian Hollfelder
Source: check_circle
Crossref

Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies

Analytical Chemistry
2023-03-14 | Journal article
DOI: 10.1021/acs.analchem.2c04144
Contributors: Elliot J. Medcalf; Maximilian Gantz; Tomasz S. Kaminski; Florian Hollfelder
Source: check_circle
Crossref

The biosynthesis, degradation, and function of cell wall β-xylosylated xyloglucan mirrors that of arabinoxyloglucan

2023-03-04 | Preprint
DOI: 10.1101/2023.03.03.528403
Contributors: L. F. L. Wilson; S. Neun; L. Yu; T. Tryfona; K. Stott; F. Hollfelder; P. Dupree
Source: check_circle
Crossref

Selection of a Promiscuous Minimalist cAMP Phosphodiesterase from a Library ofDe NovoDesigned Proteins

2023-02-14 | Preprint
DOI: 10.1101/2023.02.13.528392
Contributors: J. David Schnettler; Michael S. Wang; Maximilian Gantz; Christina Karas; Florian Hollfelder; Michael H. Hecht
Source: check_circle
Crossref

Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture

Nature Communications
2023-02-11 | Journal article
DOI: 10.1038/s41467-023-36099-7
Contributors: Philipp Knyphausen; Mariana Rangel Pereira; Paul Brear; Marko Hyvönen; Lutz Jermutus; Florian Hollfelder
Source: check_circle
Crossref

Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture

2023-01-19 | Preprint
DOI: 10.1101/2023.01.19.524706
Contributors: Philipp Knyphausen; Mariana Rangel-Pereira; Paul Brear; Marko Hyvönen; Lutz Jermutus; Florian Hollfelder
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Crossref

Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase

Journal of the American Chemical Society
2023-01-18 | Journal article
DOI: 10.1021/jacs.2c10673
Contributors: J. David Schnettler; Oskar James Klein; Tomasz S. Kaminski; Pierre-Yves Colin; Florian Hollfelder
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High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform

Analytical Chemistry
2022-12-06 | Journal article
DOI: 10.1021/acs.analchem.2c03164
Contributors: Stefanie Neun; Liisa van Vliet; Florian Hollfelder; Fabrice Gielen
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Crossref

Functional metagenomic screening identifies an unexpected β-glucuronidase

Nature Chemical Biology
2022-10 | Journal article
DOI: 10.1038/s41589-022-01071-x
Contributors: Stefanie Neun; Paul Brear; Eleanor Campbell; Theodora Tryfona; Kamel El Omari; Armin Wagner; Paul Dupree; Marko Hyvönen; Florian Hollfelder
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Microgel culture and spatial identity mapping elucidate the signalling requirements for primate epiblast and amnion formation

Development
2022-10-15 | Journal article
DOI: 10.1242/dev.200263
Contributors: Clara Munger; Timo N. Kohler; Erin Slatery; Anna L. Ellermann; Sophie Bergmann; Christopher A. Penfold; Ioakeim Ampartzidis; Yutong Chen; Florian Hollfelder; Thorsten E. Boroviak
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Crossref

Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension

Nature Cell Biology
2022-09 | Journal article
DOI: 10.1038/s41556-022-00984-y
Contributors: Min Bao; Jake Cornwall-Scoones; Estefania Sanchez-Vasquez; Andy L. Cox; Dong-Yuan Chen; Joachim De Jonghe; Shahriar Shadkhoo; Florian Hollfelder; Matt Thomson; David M. Glover et al.
Source: check_circle
Crossref

Ultrahigh-throughput Absorbance Activated Droplet Sorting (UHT-AADS) for enzyme screening at kilohertz frequencies

2022-09-13 | Preprint
DOI: 10.1101/2022.09.13.507731
Contributors: Elliot J. Medcalf; Maximilian Gantz; Tomasz S. Kaminski; Florian Hollfelder
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Gigavalent Display of Proteins on Monodisperse Polyacrylamide Hydrogels as a Versatile Modular Platform for Functional Assays and Protein Engineering

ACS Central Science
2022-08-24 | Journal article
DOI: 10.1021/acscentsci.2c00576
Contributors: Thomas Fryer; Joel David Rogers; Christopher Mellor; Timo N. Kohler; Ralph Minter; Florian Hollfelder
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High throughput steady-state enzyme kinetics measured in a parallel droplet generation and absorbance detection platform

2022-07-31 | Preprint
DOI: 10.1101/2022.07.28.500969
Contributors: Stefanie Neun; Liisa van Vliet; Florian Hollfelder; Fabrice Gielen
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Ultrahigh-throughput screening in microfluidic droplets: a faster route to new enzymes

Trends in Biochemical Sciences
2022-05 | Journal article
DOI: 10.1016/j.tibs.2021.11.001
Contributors: Maximilian Gantz; Godwin A. Aleku; Florian Hollfelder
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Enzymatic N-Allylation of Primary and Secondary Amines Using Renewable Cinnamic Acids Enabled by Bacterial Reductive Aminases

ACS Sustainable Chemistry & Engineering
2022-05-23 | Journal article
DOI: 10.1021/acssuschemeng.2c01180
Contributors: Godwin A. Aleku; Gabriel R. Titchiner; George W. Roberts; Sasha R. Derrington; James R. Marshall; Florian Hollfelder; Nicholas J. Turner; David Leys
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Functional metagenomic screening in microfluidic droplets identifies a β-glucuronidase in an unprecedented sequence neighbourhood

2022-04-25 | Preprint
DOI: 10.1101/2022.04.25.489410
Contributors: Stefanie Neun; Paul Brear; Eleanor Campbell; Theodora Tryfona; Kamel El Omari; Armin Wagner; Paul Dupree; Marko Hyvönen; Florian Hollfelder
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Ultrahigh-throughput directed evolution of a metal-free α/β-hydrolase with a Cys-His-Asp triad into an efficient phosphotriesterase

2022-02-14 | Preprint
DOI: 10.1101/2022.02.14.480337
Contributors: David Schnettler Fernández; Oskar James Klein; Tomasz S. Kaminski; Pierre-Yves Colin; Florian Hollfelder
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Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates

Nature Communications
2022-02-11 | Journal article
DOI: 10.1038/s41467-022-28396-4
Contributors: Remkes A. Scheele; Laurens H. Lindenburg; Maya Petek; Markus Schober; Kevin N. Dalby; Florian Hollfelder
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Growth amplification in ultrahigh-throughput microdroplet screening increases sensitivity of clonal enzyme assays and minimizes phenotypic variation

Lab on a Chip
2021 | Journal article
DOI: 10.1039/D0LC00830C
Contributors: Paul Jannis Zurek; Raphaëlle Hours; Ursula Schell; Ahir Pushpanath; Florian Hollfelder
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Ultrahigh‐Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay

ChemBioChem
2021-12-02 | Journal article
DOI: 10.1002/cbic.202100322
Contributors: Miriam Klaus; Paul Jannis Zurek; Tomasz S. Kaminski; Ahir Pushpanath; Katharina Neufeld; Florian Hollfelder
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Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats

Proceedings of the National Academy of Sciences
2021-11-16 | Journal article
DOI: 10.1073/pnas.2017708118
Contributors: Laurens H. Lindenburg; Teodors Pantelejevs; Fabrice Gielen; Pedro Zuazua-Villar; Maren Butz; Eric Rees; Clemens F. Kaminski; Jessica A. Downs; Marko Hyvönen; Florian Hollfelder
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A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution

ACS Synthetic Biology
2021-08-20 | Journal article
DOI: 10.1021/acssynbio.1c00084
Contributors: Che Fai Alex Wong; Liisa van Vliet; Swapnil Vilas Bhujbal; Chengzhi Guo; Marit Sletmoen; Bjørn Torger Stokke; Florian Hollfelder; Rahmi Lale
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TRIAD: a transposition-based approach for gene mutagenesis by random short in-frame insertions and deletions for directed protein evolution

2021-06-14 | Other
DOI: 10.21203/rs.3.pex-1448/v1
Contributors: Stephane Emond; Florian Hollfelder
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Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naïve Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation

Angewandte Chemie
2021-05-03 | Journal article
DOI: 10.1002/ange.202015834
Contributors: George S. Biggs; Oskar James Klein; Sarah L. Maslen; J. Mark Skehel; Trevor J. Rutherford; Stefan M. V. Freund; Florian Hollfelder; Sally R. Boss; Paul D. Barker
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Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naïve Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation

Angewandte Chemie International Edition
2021-05-03 | Journal article
DOI: 10.1002/anie.202015834
Contributors: George S. Biggs; Oskar James Klein; Sarah L. Maslen; J. Mark Skehel; Trevor J. Rutherford; Stefan M. V. Freund; Florian Hollfelder; Sally R. Boss; Paul D. Barker
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“NAD‐display”: Ultrahigh‐Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry

Angewandte Chemie
2021-04-12 | Journal article
DOI: 10.1002/ange.202013486
Contributors: Laurens Lindenburg; Florian Hollfelder
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“NAD‐display”: Ultrahigh‐Throughput in Vitro Screening of NAD(H) Dehydrogenases Using Bead Display and Flow Cytometry

Angewandte Chemie International Edition
2021-04-12 | Journal article
DOI: 10.1002/anie.202013486
Contributors: Laurens Lindenburg; Florian Hollfelder
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Crossref

Cell-free Directed Evolution of a Protease in Microdroplets at Ultrahigh Throughput

ACS Synthetic Biology
2021-02-19 | Journal article
DOI: 10.1021/acssynbio.0c00538
Contributors: Josephin M. Holstein; Christian Gylstorff; Florian Hollfelder
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Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays

Analytical Chemistry
2021-02-02 | Journal article
DOI: 10.1021/acs.analchem.0c03992
Contributors: Tuomas Huovinen; Laurens Lindenburg; Ralph Minter; Florian Hollfelder
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Microfluidic Droplets and Their Applications: Diagnosis, Drug Screening and the Discovery of Therapeutic Enzymes

2020 | Book chapter
DOI: 10.1007/978-981-13-5859-3_63
Contributors: Liisa D. van Vliet; Florian Hollfelder
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Items per page:
Page 1 of 4

Peer review (10 reviews for 7 publications/grants)

Review activity for ACS catalysis. (2)
Review activity for ACS central science. (1)
Review activity for ACS chemical biology. (2)
Review activity for ACS sustainable chemistry & engineering. (1)
Review activity for Angewandte Chemie. (2)
Review activity for Journal of the American Chemical Society. (1)
Review activity for Nature communications (1)
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